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DR1123 Anti-GCM1 Mouse mAb (2E11)

Anti-GCM1, mouse monoclonal, clone 2E11, recognizes the ~50 kDa GCM1 in FHs 173 WE and HeLa cells and human stomach tissue. It is validated for use in ELISA, WB, ICC, and IHC on paraffin sections.

Anti-GCM1 Mouse mAb (2E11) MSDS (material safety data sheet) or SDS, CoA and CoQ, dossiers, brochures and other available documents.

Synonyms: Anti-Glial Cells Missing Homolog 1

DR1123
  
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Overview

Replacement Information

Key Spec Table

Species ReactivityHostAntibody Type
HMMonoclonal Antibody
Description
OverviewRecognizes the ~50 kDa GCM1 protein in FHs 173 WE and HeLa cells and human stomach tissue.
Catalogue NumberDR1123
Brand Family Calbiochem®
SynonymsAnti-Glial Cells Missing Homolog 1
Application Data
Detection of human GCM1 by immunoblotting. Sample: Whole cell lysate from FHs 173 WE cells. Primary antibody: Anti-GCM1 Mouse mAb (2E11) (Cat. No. DR1123) (1-5 µg/ml). Detection: chemiluminescence.

Detection of human GCM1 by immunocytochemistry. Sample: HeLa cells fixed in paraformaldehyde. Primary antibody: Anti-GCM1 Mouse mAb (2E11) (Cat. No. DR1123) (10 µg/ml). Detection: fluorescence.

Detection of human GCM1 by staining paraffin sections. Sample: Formalin-fixed, paraffin-embedded human stomach tissue. Primary antibody: Anti-GCM1 Mouse mAb (2E11) (Cat. No. DR1123) (1.7 µg/ml). Detection: colorimetric.
References
Product Information
Formliquid
FormulationIn PBS, pH 7.2.
Positive controlFHs 173 WE cells, Human stomach tissue, HeLa cells
PreservativeNone
Quality LevelMQ100
Applications
Key Applications Enzyme-Linked Immunosorbent Assay
Immunoblotting (Western Blotting)
Immunocytochemistry
Paraffin Sections
Application NotesELISA (see comments) Immunoblotting (1-5 µg/ml) Immunocytochemistry (10 µg/ml) Paraffin Sections (1.7 µg/ml)
Application CommentsFor ELISA, this antibody can be used as the capture antibody; recommended concentration is 10 ng/ml, 1 µg/well, 100 µl total volume. Variables associated with assay conditions will dictate the proper working dilution.
Biological Information
ImmunogenA recombinant polypeptide corresponding to amino acids of 108-166 human GCM1, expressed as a GST fusion protein
Clone2E11
HostMouse
IsotypeIgG2a
Species Reactivity
  • Human
Antibody TypeMonoclonal Antibody
Physicochemical Information
Dimensions
Materials Information
Toxicological Information
Safety Information according to GHS
Safety Information
Product Usage Statements
Storage and Shipping Information
Ship Code Blue Ice Only
Toxicity Regulatory Review
Storage -20°C
Avoid freeze/thaw Avoid freeze/thaw
Do not freeze Ok to freeze
Special InstructionsFollowing initial thaw, aliquot and freeze (-20°C).
Packaging Information
Transport Information
Supplemental Information
Specifications
Global Trade Item Number
Catalogue Number GTIN
DR1123 0

Documentation

Anti-GCM1 Mouse mAb (2E11) MSDS

Title

Safety Data Sheet (SDS) 

Data Sheet

Note that this data sheet is not lot-specific and is representative of the current specifications for this product. Please consult the vial label and the certificate of analysis for information on specific lots. Also note that shipping conditions may differ from storage conditions.

Revision05-November-2010 RFH
SynonymsAnti-Glial Cells Missing Homolog 1
ApplicationELISA (see comments) Immunoblotting (1-5 µg/ml) Immunocytochemistry (10 µg/ml) Paraffin Sections (1.7 µg/ml)
Application Data
Detection of human GCM1 by immunoblotting. Sample: Whole cell lysate from FHs 173 WE cells. Primary antibody: Anti-GCM1 Mouse mAb (2E11) (Cat. No. DR1123) (1-5 µg/ml). Detection: chemiluminescence.

Detection of human GCM1 by immunocytochemistry. Sample: HeLa cells fixed in paraformaldehyde. Primary antibody: Anti-GCM1 Mouse mAb (2E11) (Cat. No. DR1123) (10 µg/ml). Detection: fluorescence.

Detection of human GCM1 by staining paraffin sections. Sample: Formalin-fixed, paraffin-embedded human stomach tissue. Primary antibody: Anti-GCM1 Mouse mAb (2E11) (Cat. No. DR1123) (1.7 µg/ml). Detection: colorimetric.
DescriptionPurified mouse monoclonal antibody. Recognizes the ~50 kDa GCM1 protein.
BackgroundThis gene encodes a DNA-binding protein with a gcm-motif (glial cell missing motif). The encoded protein is a homolog of the Drosophila glial cells missing (GCM) gene. This protein binds to the GCM-motif (A/G)CCCGCAT, a novel sequence among known targets of DNA-binding proteins. The N-terminal DNA-binding domain confers the unique DNA-binding activity of this protein.
HostMouse
ImmunogenA recombinant polypeptide corresponding to amino acids of 108-166 human GCM1, expressed as a GST fusion protein
Clone2E11
IsotypeIgG2a
Specieshuman
Positive controlFHs 173 WE cells, Human stomach tissue, HeLa cells
Formliquid
FormulationIn PBS, pH 7.2.
PreservativeNone
CommentsFor ELISA, this antibody can be used as the capture antibody; recommended concentration is 10 ng/ml, 1 µg/well, 100 µl total volume. Variables associated with assay conditions will dictate the proper working dilution.
Storage Avoid freeze/thaw
-20°C
Do Not Freeze Ok to freeze
Special InstructionsFollowing initial thaw, aliquot and freeze (-20°C).
Toxicity Regulatory Review