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ABE1845 Anti-GABPA Antibody

Detect GABPA using this Anti-GABPA Antibody validated for use in Western Blotting and Chromatin Immunoprecipitation (ChIP).

Anti-GABPA Antibody MSDS (material safety data sheet) or SDS, CoA and CoQ, dossiers, brochures and other available documents.
Research Category: Epigenetics & Nuclear Function Research Sub-Category: Nuclear Receptors Gene Symbol: Gabpa, E4tf1a UniProt Number: Q00422
ABE1845
200 μg  
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Key Spec Table

Species ReactivityKey ApplicationsHostFormatAntibody Type
M, H, RWB, ChIPRbPurifiedPolyclonal Antibody
Description
Catalogue NumberABE1845
DescriptionAnti-GABPA Antibody
Alternate Names
  • GA-binding protein alpha chain
  • GABP subunit alpha
  • GABPA
Background InformationGA-binding protein alpha chain (UniProt Q00422; also known as GA repeat binding protein alpha, GABP subunit alpha, GABPalpha, NRF-2, Nuclear respiratory factor-2) is encoded by the Gabpa gene (also known as E4tf1a) in murine species (Gene ID 14390). GABPalpha is a redox-responsive transcription factor that plays an anti-oxidant, pro-survival role in mitochondrial biogenesis process. GABPalpha is upregulated in response to oxidant exposure or increased cellular reactive oxygen species (ROS), where it mediates the transcription of mitochondrial transcription factor A (mtTFA) that is needed for mitochondrial DNA transcription.
References
Product Information
FormatPurified
PresentationPurified rabbit polyclonal antibody in buffer containing PBS and 0.01% Sodium Azide.
Quality LevelMQ100
Applications
ApplicationDetect GABPA using this Anti-GABPA Antibody validated for use in Western Blotting and Chromatin Immunoprecipitation (ChIP).
Key Applications
  • Western Blotting
  • Chromatin Immunoprecipitation (ChIP)
Application NotesWestern Blotting Analysis: A representative lot detected GABPA in mouse liver nuclear proteins (Cherry, A.D., et al. (2014). J. Biol. Chem. 289(1):41-52).
Chromatin Immunoprecipitation Analysis: A representative lot detected GABPA occupancy at the Sod2 promoter region by ChIP using liver chromatin preparation from mice after S. aureus inoculation (Cherry, A.D., et al. (2014). J. Biol. Chem. 289(1):41-52).
Biological Information
ImmunogenKLH-conjugated linear peptide corresponding to mouse GABPA near the C-terminus.
EpitopeNear C-terminus
ConcentrationPlease refer to lot specific datasheet.
HostRabbit
Species Reactivity
  • Mouse
  • Human
  • Rat
Species Reactivity NoteMouse, Human, Rat. Predicted to react with Monkey, Bovine, Canine based on 100% sequence homology.
Antibody TypePolyclonal Antibody
Entrez Gene Number
Gene Symbol
  • Gabpa
  • E4tf1a
Purification MethodProtein A Purfied
UniProt Number
Molecular Weight~55 kDa observed
Physicochemical Information
Dimensions
Materials Information
Toxicological Information
Safety Information according to GHS
Safety Information
Product Usage Statements
Quality AssuranceEvaluated by Western Blotting in mouse liver tissue lysate.

Western Blotting Analysis: 2.0 µg/mL of this antibody detected GABPA in 10 µg of mouse liver tissue lysate.
Usage Statement
  • Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
Storage and Shipping Information
Storage ConditionsStable for 1 year at 2-8°C from date of receipt.
Packaging Information
Material Size200 μg
Transport Information
Supplemental Information
Specifications
Global Trade Item Number
Catalogue Number GTIN
ABE1845 04055977173055

Documentation

Anti-GABPA Antibody MSDS

Title

Safety Data Sheet (SDS) 

Anti-GABPA Antibody Certificates of Analysis

TitleLot Number
Anti-GABPA - 3290321 3290321
Anti-GABPA - 3761770 3761770
Anti-GABPA - 3862584 3862584
Anti-GABPA - 3933280 3933280
Anti-GABPA -Q2573216 Q2573216

Technical Info

Title
Characterization of Estrogen Receptor α Phosphorylation Sites in Breast Cancer Tissue Using the SNAP i.d® 2.0 System
White Paper - The Message in the Marks: Deciphering Cancer Epigenetics
White Paper: Further considerations of antibody validation and usage.