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MAB1678-I-25UL Anti-Filamin A Antibody, clone PM6/317

Anti-Filamin A, clone PM6/317, Cat. No. MAB1678-I, is a mouse monoclonal antibody that detects Filamin-A and is tested for use in Flow Cytometry, Immunocytochemistry, Immunohistochemistry (Paraffin), and Western Blotting.

MAB1678-I-25UL
25 µL  
Purchase on Sigma-Aldrich

Overview

Replacement Information
Description
Catalogue NumberMAB1678-I-25UL
DescriptionAnti-Filamin A Antibody, clone PM6/317
Alternate Names
  • Filamin-A
  • FLN-A
  • Actin-binding protein 280
  • ABP-280
  • Alpha-filamin
  • Endothelial actin-binding protein
  • Filamin-1
  • Non-muscle filamin
Background InformationFilamin-A (UniProt: P21333; also known as FLN-A, Actin-binding protein 280, ABP-280, Alpha-filamin, Endothelial actin-binding protein, Filamin-1, Non-muscle filamin) is encoded by the FLNA (also known as FLN, FLN1) gene (Gene ID: 2316) in human. Filamin-A is a ubiquitous cytoskeletal homodimeric protein that associates with actin filaments in the platelet cytoskeleton and links this structure to other structural/signaling proteins such as -actinin and to membrane receptors such as the vWF receptor (GPIb-IX-V). It is also expressed in migrating neurons in the prenatal neocortex. Each Filamin-A monomer contains an N-terminal actin-binding domain, which is followed by 24 tandem immunoglobulin (Ig)-like domains interrupted by two flexible hinges. The two monomers dimerize through their C-terminal domain to generate the homodimeric form. Repeat 24 and the second hinge domain are shown to be important for dimer formation. Filamin repeat 20 interacts with filamin repeat 21 masking the ligand binding site on filamin repeat 21, which results in an autoinhibited conformation. This autoinhibition can be relieved by ligands like ITGB7 or FBLIM1. Filamin-A promotes orthogonal branching of actin filaments and links actin filaments to membrane glycoproteins. It anchors various transmembrane proteins to the actin cytoskeleton and serves as a scaffold for a wide range of cytoplasmic signaling proteins. It also plays a role in cell-cell contacts and adherens junctions during the development of blood vessels, heart and brain organs and plays a role in platelets morphology through interaction with SYK that regulates ITAM- and ITAM-like-containing receptor signaling. During the axon guidance process, Filamin-A is required for growth cone collapse induced by SEMA3A-mediated stimulation of neurons. Its phosphorylation at serine 2152 is reported to be negatively regulated by its autoinhibited conformation.
References
Product Information
FormatPurified
PresentationPurified mouse monoclonal antibody IgG1 in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide.
Quality LevelMQ200
Applications
ApplicationAnti-Filamin A, clone PM6/317, Cat. No. MAB1678-I, is a mouse monoclonal antibody that detects Filamin-A and is tested for use in Flow Cytometry, Immunocytochemistry, Immunohistochemistry (Paraffin), and Western Blotting.
Key Applications
  • Western Blotting
  • Immunocytochemistry
  • Immunohistochemistry (Paraffin)
  • Flow Cytometry
Application NotesImmunocytochemistry Analysis: A 1:500 dilution from a representative lot detected Filamin A in HeLa cells.

Flow Cytometry Analysis: 1 µg from a representative lot detected Filamin A in A431 cells.

Western Blotting Analysis: A representative lot detected Filamin A in Western Blotting applications (Fox, J.W., et. al. (1998). Neuron. 21(6):1315-25; McDowall, A., et. al. (2003). J Clin Invest. 111(1):51-60; Umeda, T., et. al. (2001). J Biochem. 130(4):535-42).

Immunohistochemistry (Paraffin) Analysis: A 1:250 dilution from a representative lot detected Filamin A in human prostate and human kidney tissue sections.

Note: Actual optimal working dilutions must be determined by end user as specimens, and experimental conditions may vary with the end user
Biological Information
ImmunogenFilamin-A from human platelets.
EpitopeUnknown
ClonePM6/317
Concentration0.5 ug/mL. Please refer to guidance on suggested starting dilutions and/or titers per application and sample type.
HostMouse
SpecificityClone PM6/317 is a mouse monoclonal antibody that detects Filamin-A in several vertebrate species.
IsotypeIgG1κ
Species Reactivity
  • Human
Species Reactivity NoteHuman. Predicted to react with Chicken, Guinea Pig, Mouse, Rabbit, Rat based on 100% sequence homology.
Antibody TypeMonoclonal Antibody
Entrez Gene Number
Gene Symbol
  • FLNA
  • FLN
  • FLN1
Purification MethodProtein G purified
UniProt Number
Molecular Weight~190 and ~280 kDa observed; 280.74 kDa calculated. Uncharacterized bands may be observed in some lysate(s).
Physicochemical Information
Dimensions
Materials Information
Toxicological Information
Safety Information according to GHS
Safety Information
Product Usage Statements
Quality AssuranceEvaluated by Western Blotting in Jurkat cell lysate.

Western Blotting Analysis: A 1:1,000 dilution of this antibody detected Filamin A in Jurkat cell lysate.
Usage Statement
  • Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
Storage and Shipping Information
Storage ConditionsStable for 1 year at +2°C to +8°C from date of receipt.
Packaging Information
Material Size25 µL
Transport Information
Supplemental Information
Specifications
Global Trade Item Number
Catalogue Number GTIN
MAB1678-I-25UL 04065265398046