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MABC1110-25UG Anti-FGFR4 Antibody, clone BT53

Anti-FGFR4, clone BT53, Cat. No. MABC1110, is a mouse monoclonal antibody that detects FGFR4 and is tested for use in ELISA, Flow Cytometry, Immunocytochemistry, Immunotoxin, and Inhibition Assay.

MABC1110-25UG
25 μg  
Purchase on Sigma-Aldrich

Overview

Replacement Information
Description
Catalogue NumberMABC1110-25UG
DescriptionAnti-FGFR4 Antibody, clone BT53
Alternate Names
  • Fibroblast growth factor receptor 4
  • EC: 2.7.10.1
  • CD334
Background InformationFibroblast growth factor receptor 4 (UniProt: P22455; also known as EC: 2.7.10.1, FGFR-4, CD334) is encoded by the FGRF4 (also known as JTK2, TKF) gene (Gene ID: 2264) in human. FGFR-4 is a highly conserved, single-pass type I membrane protein with tyrosine kinase activity that serves as a cell surface receptor for fibroblast growth factors and regulates cell proliferation differentiation, and migration. FGFR-4 is expressed in gastrointestinal epithelial cells, pancreas, and gastric and pancreatic cancer cell lines. In the absence of a ligand, it is present as a monomer in an inactive conformation. Upon ligand binding it homodimerizes into an active conformation with autophosphorylation of tyrosine residues. FGFR-4 is synthesized with a signal peptide (aa 1-21), which is subsequently cleaved off in the mature form. It contains an extracellular domain (aa 22-369), a transmembrane domain (aa 370-390), and a cytoplasmic domain (aa 391-802). FGFR-4 undergoes N-glycosylation, and when not fully glycosylated, it can undergo proteasomal degradation. FGFR-4 signaling is down-regulated by receptor internalization and degradation. MMP14 is reported to promote internalization and degradation of FGFR-4. Mutations that lead to constitutive kinase activation or impair normal FGFR-4 inactivation lead to aberrant signaling leading to tumor growth. Over-expression of FGFR4 is reported in almost all human prostate cancers and its R388G mutation is associated with tumor progression. It leads to increased receptor stability and sustained receptor activation. (Ref.: Turkington, RC et al. (2014). Cell Death Dis. 5; e1046; Wang, J., et al. (2008). Neoplasia. 10(8); 847-856).
References
Product Information
FormatPurified
PresentationPurified mouse monoclonal antibody IgG2b in PBS without preservatives.
Quality LevelMQ200
Applications
ApplicationAnti-FGFR4, clone BT53, Cat. No. MABC1110, is a mouse monoclonal antibody that detects FGFR4 and is tested for use in ELISA, Flow Cytometry, Immunocytochemistry, Immunotoxin, and Inhibition Assay.
Key Applications
  • Enzyme Immunoassay (ELISA)
  • Flow Cytometry
  • Immunocytochemistry
  • Inhibition
Application NotesTested Applications

ELISA Analysis: A representative lot detected Fibroblast growth factor receptor 4 (FGFR4) in ELISA applications.

Inhibition Assay:: A representative lot inhibited growth, replication, and metastasis in RH30 cells.

Immunotoxin: A representative lot of this antibody displayed immunotoxin activity as antibody-drug conjugate with Monomethyl auristatin F or Duocamycin DM.

Flow Cytometry Analysis: A representative lot detected Fibroblast growth factor receptor 4 (FGFR4) in Flow Cytometry applications.

Note: Actual optimal working dilutions must be determined by end user as specimens, and experimental conditions may vary with the end user.
Biological Information
ImmunogenCells transfected with human FGFR4.
EpitopeExtracellular domain
CloneBT53
Concentration1.0 mg/mL. Please refer to guidance on suggested starting dilutions and/or titers per application and sample type.
HostMouse
SpecificityClone BT53 is a mouse monoclonal antibody that detects FGFR4. It targets an epitope within the extracellular domain.
IsotypeIgG2bκ
Species Reactivity
  • Human
Species Reactivity NoteHuman.
Antibody TypeMonoclonal Antibody
Entrez Gene Number
Gene Symbol
  • FGFR4
Purification MethodProtein G purified
UniProt Number
Molecular Weight87.95 kDa calculated.
Physicochemical Information
Dimensions
Materials Information
Toxicological Information
Safety Information according to GHS
Safety Information
Product Usage Statements
Quality AssuranceEvaluated by Immunocytochemistry in HepG2 cells.

Immunocytochemistry Analysis: A 1:250 dilution of this antibody detected Fibroblast growth factor receptor 4 (FGFR4) in HepG2 cells.
Usage Statement
  • Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
Storage and Shipping Information
Storage ConditionsStore at -10°C to -25°C. Handling Recommendations: Upon receipt and prior to removing the cap, centrifuge the vial and gently mix the solution. Aliquot into microcentrifuge tubes and store at -20°C. Avoid repeated freeze/thaw cycles, which may damage IgG and affect product performance.
Packaging Information
Material Size25 μg
Transport Information
Supplemental Information
Specifications
Global Trade Item Number
Catalogue Number GTIN
MABC1110-25UG 04065270453518