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MABC1704-100UG Anti-Eotaxin Antibody, clone 13H2

Anti-Eotaxin, clone 13H2, Cat. No. MABC1704, is a mouse monoclonal antibody that detects Eotaxin and has been tested for use in Immunohistochemistry (Paraffin) and Western Blotting.

MABC1704-100UG
100 μg  
Purchase on Sigma-Aldrich

Overview

Replacement Information
Description
Catalogue NumberMABC1704-100UG
DescriptionAnti-Eotaxin Antibody, clone 13H2
Alternate Names
  • C-C motif chemokine 11
  • Eosinophil chemotactic protein
  • Small-inducible cytokine A11
Background InformationEotaxin (UniProt: P51671; also known as C-C motif chemokine 11, Eosinophil chemotactic protein, Small-inducible cytokine A11) is encoded by the CCL11 (also known as SCYA11) gene (Gene ID: 6356) in human. Eotaxin is a potent and eosinophil-specific chemoattractant that plays a critical role in allergic and nonallergic inflammatory reactions. In response to the presence of allergens it directly promotes the accumulation of eosinophils at the site of inflammation. It is constitutively expressed in several tissues including intestine, lymphoid node, thymus, skin, heart, kidney, and mammary gland. It is a secreted protein that is synthesized with a signal peptide (aa 1-23), which is subsequently cleaved off to produce the mature form. Eotaxin is shown to be induced by TNFa, IL-1a, and IFN-g. In addition to its role in immune response, Eotaxin is also associated with aging, neurogenesis and neurodegeneration and can influence neural progenitor cells, and microglia. Its elevated circulating levels have been shown in several psychiatric disorders, including schizophrenia, bipolar disorder, and major depression. (Ref.: Teixeira, AL., et al. (2018). Front Psychiatry. 9; 241; Salcedo, R., et al. (2001). J. Immunol. 166(12); 7571-7578).
References
Product Information
FormatPurified
PresentationPurified mouse monoclonal antibody IgG1 in PBS with 0.05% sodium azide.
Quality LevelMQ200
Applications
ApplicationAnti-Eotaxin, clone 13H2, Cat. No. MABC1704, is a mouse monoclonal antibody that detects Eotaxin and has been tested for use in Immunohistochemistry (Paraffin) and Western Blotting.
Key Applications
  • Western Blotting
  • Immunohistochemistry (Paraffin)
Application NotesImmunohistochemistry (Paraffin) Analysis: A 1:50 dilution from a representative lot detected Eotaxin in human skin and human small intestine tissue sections.

Note: Actual optimal working dilutions must be determined by end user as specimens, and experimental conditions may vary with the end user
Biological Information
ImmunogenFull-length recombinant mature human Eotaxin
EpitopeUnknown
Clone13H2
Concentration1 mg/mL. Please refer to guidance on suggested starting dilutions and/or titers per application and sample type.
HostMouse
SpecificityClone 13H2 is a mouse monoclonal antibody that detects human Eotaxin.
IsotypeIgG1κ
Species Reactivity
  • Human
Species Reactivity NoteHuman.
Antibody TypeMonoclonal Antibody
Entrez Gene Number
Gene Symbol
  • CCL11
  • SCYA11
Purification MethodProtein G purified
UniProt Number
Molecular Weight~12 kDa observed; 10.73 kDa calculated. Uncharacterized bands may be observed in some lysate(s).
Physicochemical Information
Dimensions
Materials Information
Toxicological Information
Safety Information according to GHS
Safety Information
Product Usage Statements
Quality AssuranceEvaluated by Western Blotting with human recombinant Eotaxin.

Western Blotting Analysis: 1:50,000 dilution of this antibody detected recombinant human Eotaxin.
Usage Statement
  • Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
Storage and Shipping Information
Storage ConditionsStable for 1 year at +2°C to +8°C from date of receipt.
Packaging Information
Material Size100 μg
Transport Information
Supplemental Information
Specifications
Global Trade Item Number
Catalogue Number GTIN
MABC1704-100UG 04061841879855