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MABF985 Anti-Eosinophil-Derived Neurotoxin Antibody, clone 6D1.5/A5

Anti-Eosinophil-Derived Neurotoxin Antibody, clone 6D1.5/A5 is an antibody against Eosinophil-Derived Neurotoxin for use in Western Blotting, ELISA, Multiplexing.

Anti-Eosinophil-Derived Neurotoxin Antibody, clone 6D1.5/A5 MSDS (material safety data sheet) or SDS, CoA and CoQ, dossiers, brochures and other available documents.
Research Category: Inflammation & Immunology Research Sub-Category: Inflammation & Autoimmune Mechanisms Gene Symbol: RNASE2, EDN, RAF3, RNS2 UniProt Number: P10153
MABF985
100 μg  
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Key Spec Table

Species ReactivityKey ApplicationsHostFormatAntibody Type
HWB, ELISA, MplexMPurifiedMonoclonal Antibody
Description
Catalogue NumberMABF985
DescriptionAnti-Eosinophil-Derived Neurotoxin Antibody, clone 6D1.5/A5
Alternate Names
  • Non-secretory ribonuclease
  • Eosinophil-derived neurotoxin
  • Ribonuclease 2
  • Ribonuclease US
  • RNase 2
  • RNase UpI-2
Background InformationNon-secretory ribonuclease (EC 3.1.27.5; UniProt P10153; also known as Eosinophil-derived neurotoxin, Ribonuclease 2, Ribonuclease US, RNase 2, RNase UpI-2) is encoded by the RNASE2 (also known as EDN, RAF3, RNS2) gene (Gene ID 6036) in human. The major basic protein (MBP), eosinophil cationic protein (ECP), eosinophil derived neurotoxin (EDN) and eosinophil peroxidase (EPO), are eosinophil effector molecules implicated in host defense, immunoregulatory responses, and in allergic and inflammatory reactions. These highly cationic proteins are stored preformed within the eosinophil secondary granules and released in intact membrane-bound granules during cell lysis or through a complex system of vesiculotubular structures during “piecemeal necrosis”. While eosinophil count and eosinophil surface expression of the activation marker CD69 are significantly correlated with serum concentrations of MBP, EDN and EPO, studies show that they are not correlated with ECP level. On the other hand, eosinophilic disease patients with normal eosinophil count show significantly increased concentrations of MBP, ECP, EDN, and EPO compared to normal donors. The measurement of MBP, ECP, EDN, and EPO levels thus provides a better predictor than eosinophil count alone in disease diagnosis. EDN is produced with a signal peptide sequence (a.a. 1-27), the removal of which yields the mature (a.a. 28-161) protein.
References
Product Information
FormatPurified
PresentationPurified mouse monoclonal IgG1 antibody in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide.
Quality LevelMQ100
Applications
ApplicationAnti-Eosinophil-Derived Neurotoxin Antibody, clone 6D1.5/A5 is an antibody against Eosinophil-Derived Neurotoxin for use in Western Blotting, ELISA, Multiplexing.
Key Applications
  • Western Blotting
  • ELISA
  • Multiplexing
Application NotesWestern Blotting Analysis: 1.0 µg/mL from a representative lot detected eosinophil-derived neurotoxin in 50 µg of human liver and lung tissue lysates.
ELISA Analysis: A representative lot was employed as the capture antibody for the detection of purified human EDN, as well as EDN in serum samples from individuals with eosinophilic disorders by sandwich ELISA (Makiya, M.A., et al. (2014). J. Immunol. Methods. 411:11-22),
Multiplexing Analysis: A representative lot was employed as the capture antibody in a bead-based multiplex assay for the detection of purified human EDN as well as EDN in serum samples from healthy donors and individuals with eosinophilic disorders. Prior reduction and alkylation of the serum samples (by DTT and iodoacetamide, respectively) resulted in higher EDN levels (Makiya, M.A., et al. (2014). J. Immunol. Methods. 411:11-22),
Biological Information
ImmunogenPurified human EDN.
Clone6D1.5/A5
ConcentrationPlease refer to lot specific datasheet.
HostMouse
SpecificityClone 6D1.5/A5 specifically reacted with EDN, but not other granule proteins by Western blotting analysis (Makiya, M.A., et al. (2014). J. Immunol. Methods. 411:11-22).
IsotypeIgG1
Species Reactivity
  • Human
Antibody TypeMonoclonal Antibody
Entrez Gene Number
Gene Symbol
  • RNASE2
  • EDN
  • RAF3
  • RNS2
Purification MethodProtein G Purified
UniProt Number
Molecular Weight~20 kDa observed. Target band appears larger than the calculated molecular weights of 18.35 kDa (pro-form) and 15.46 kDa (mature) due to glycosylation. Uncharacterized band(s) may appear in some lysates.
Physicochemical Information
Dimensions
Materials Information
Toxicological Information
Safety Information according to GHS
Safety Information
Product Usage Statements
Quality AssuranceEvaluated by Western Blotting in human spleen tissue lysate.

Western Blotting Analysis: 1.0 µg/mL of this antibody detected eosinophil-derived neurotoxin in 50 µg of human spleen tissue lysate.
Usage Statement
  • Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
Storage and Shipping Information
Storage ConditionsStable for 1 year at 2-8°C from date of receipt.
Packaging Information
Material Size100 μg
Transport Information
Supplemental Information
Specifications
Global Trade Item Number
Catalogue Number GTIN
MABF985 04055977334425

Documentation

Anti-Eosinophil-Derived Neurotoxin Antibody, clone 6D1.5/A5 MSDS

Title

Safety Data Sheet (SDS) 

Anti-Eosinophil-Derived Neurotoxin Antibody, clone 6D1.5/A5 Certificates of Analysis

TitleLot Number
Anti-Eosinophil-Derived Neurotoxin, -2792900 2792900
Anti-Eosinophil-Derived Neurotoxin, -2795635 2795635
Anti-Eosinophil-Derived Neurotoxin, -2807719 2807719
Anti-Eosinophil-Derived Neurotoxin, -Q2668632 Q2668632
Anti-Eosinophil-Derived Neurotoxin, clone 6D1.5/A5 - 3200634 3200634
Anti-Eosinophil-Derived Neurotoxin, clone 6D1.5/A5 - 3508321 3508321
Anti-Eosinophil-Derived Neurotoxin, clone 6D1.5/A5 - 4016279 4016279
Anti-Eosinophil-Derived Neurotoxin, clone 6D1.5/A5 Monoclonal Antibody 3091259
Anti-Eosinophil-Derived Neurotoxin, clone 6D1.5/A5 Monoclonal Antibody 3035071
Anti-Eosinophil-Derived Neurotoxin, clone 6D1.5/A5_2840391 2840391