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MABF2294-100UG Anti-EGF clone 7C9.14.6

Anti-EGF clone 7C9.14.6, Cat. No. MABF2294, is a mouse monoclonal antibody that detects human epidermal growth factor (EGF) and has been tested for use in Affinity Binding Assay, Immunohistochemistry (Paraffin), and Western Blotting.

MABF2294-100UG
100 μg  
Purchase on Sigma-Aldrich

Overview

Replacement Information
Description
Catalogue NumberMABF2294-100UG
DescriptionAnti-EGF clone 7C9.14.6
Alternate Names
  • Urogastrone
Background InformationEpidermal growth factor (UniProt: P01133; also known as Urogastrone) is encoded by the EGF gene (Gene ID: 1950) in human. Epidermal growth factor is a single-pass type I membrane protein that is expressed in kidney, salivary gland, cerebrum, and prostate. It stimulates the growth of various epidermal and epithelial tissues in vivo and in vitro and of some fibroblasts in cell culture. It exerts its effects by binding to epidermal growth factor receptor (EGFR) that undergoes homo- or heterodimerization with erbB2 receptors, causing autophosphorylation of its tyrosine residues that in turn activate PI3K, MAPK, or phospholipase C-gamma signaling cascades. EGF is shown to stimulate magnesium reabsorption in the renal distal convoluted tubule via engagement of EGFR and activation of the magnesium channel TRPM6. EGF is synthesized with a signal peptide (aa 1-22) that is subsequently cleaved off to generate the mature form that contains a long extracellular domain (aa 23-1032), a transmembrane domain (aa 1033-1053), and a cytoplasmic domain (aa 1054-1207). Three isoforms of EGF has been described that are produced by alternative splicing. Mutations in EGF gene have been linked to hypomagnesemia that is characterized by massive renal hypomagnesemia and normal levels of serum calcium and calcium excretion, which leads to seizures and mild-to moderate psychomotor retardation.
References
Product Information
FormatPurified
PresentationPurified mouse monoclonal antibody IgG1 in PBS with 0.05% sodium azide.
Applications
ApplicationAnti-EGF clone 7C9.14.6, Cat. No. MABF2294, is a mouse monoclonal antibody that detects human epidermal growth factor (EGF) and has been tested for use in Affinity Binding Assay, Immunohistochemistry (Paraffin), and Western Blotting.
Key Applications
  • Immunohistochemistry (Paraffin)
  • Affinity Binding Assay
  • Western Blotting
Application NotesImmunohistochemistry (Paraffin) Analysis: A 1:50 dilution of this antibody detected epidermal growth factor in human kidney tissue sections.

Affinity binding assay: The binding of Anti-EGF clone 7C9.14.6, Cat. No. MABF2294 to EGF protein showed a KD value of 1.99 x10-6 whereas binding of GST showed a KD of 1.96 x 10-2.
Biological Information
ImmunogenA recombinant fragment corresponding to 53 amino acids from the C-terminal region of human Epidermal growth factor.
EpitopeC-terminus
Clone7C9.14.6
ConcentrationPlease refer to lot specific datasheet.
HostMouse
SpecificityClone 7C9.14.6 is a mouse monoclonal antibody that detects human epidermal growth factor. It targets an epitope within 53 amino acids from the C-terminal, extracellular domain region.
IsotypeIgG1κ
Species Reactivity
  • Human
Species Reactivity NoteHuman.
Antibody TypeMonoclonal Antibody
Entrez Gene Number
Gene Symbol
  • EGF
Purification MethodProtein G purified
UniProt Number
Molecular Weight133.99 kDa calculated.
Physicochemical Information
Dimensions
Materials Information
Toxicological Information
Safety Information according to GHS
Safety Information
Product Usage Statements
Quality AssuranceEvaluated by Western Blotting with human recombinant epidermal growth factor.

Western Blotting Analysis: A 1:50,000 dilution of this antibody detected human recombiant epidermal growth factor.
Usage Statement
  • Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
Storage and Shipping Information
Storage ConditionsStable for 1 year at 2-8°C from date of receipt.
Packaging Information
Material Size100 μg
Transport Information
Supplemental Information
Specifications
Global Trade Item Number
Catalogue Number GTIN
MABF2294-100UG 04061841235927