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MAB1618-AF488 Anti-Dynein Antibody, IC, cytosolic, clone 74.1, Alexa Fluor® 488 Conjugate

Anti-Dynein Antibody, IC, cytosolic, clone 74.1, Alexa Fluor® 488 Conjugate is an antibody against Dynein for use in Immunocytochemistry.

Anti-Dynein Antibody, IC, cytosolic, clone 74.1, Alexa Fluor® 488 Conjugate MSDS (material safety data sheet) or SDS, CoA and CoQ, dossiers, brochures and other available documents.
Research Category: Cell Structure Research Sub-Category: Cytoskeletal Signaling Gene Symbol: DYNC1I1, DYNC1I2 UniProt Number: Q29RQ3, Q0III3
MAB1618-AF488
100 µL  
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Key Spec Table

Species ReactivityKey ApplicationsHostFormatAntibody Type
H, Xn, F, Sh, Dr, B, R, MICCMAlexaFluor®488Monoclonal Antibody
Description
Catalogue NumberMAB1618-AF488
DescriptionAnti-Dynein Antibody, IC, cytosolic, clone 74.1, Alexa Fluor® 488 Conjugate
Alternate Names
  • Cytoplasmic dynein 1 intermediate chain 1
  • Cytoplasmic dynein intermediate chain 1
  • Dynein intermediate chain 1, cytosolic
  • DH IC-1
  • Cytoplasmic dynein 1 intermediate chain 2
  • Cytoplasmic dynein intermediate chain 2
  • Dynein intermediate chain 2, cytosolic
  • DH IC-2
Background InformationCytoplasmic dynein 1 intermediate chain 1 (UniProt Q29RQ3; also known as Cytoplasmic dynein intermediate chain 1, Dynein intermediate chain 1, cytosolic, DH IC-1) and Cytoplasmic dynein 1 intermediate chain 2 (UniProt Q0III3; also known as Cytoplasmic dynein intermediate chain 2, Dynein intermediate chain 2, cytosolic, DH IC-2) are encoded by the DYNC1I1 gene (Gene ID 613724) and DYNC1I2 gene (Gene ID 526329) in bovine, respectively. Cytoplasmic dynein complexes are microtubules-associated retrograde transport motors. Cytoplasmic dynein 1 is the more abundant motor in cells, while cytoplasmic dynein 2 takes part in intraflagellar transport. Cytoplasmic dynein 1 is a multisubunit complex of ∼1.5 MDa composed of a homodimer of heavy chains (encoded by DYNC1H1), two intermediate chains (encoded by DYNC1I1 and DYNC1I2), light-intermediate chains (encoded by DYNC1LI1, DYNC1LI2) and light chains (encoded by DYNLT1, DYNLT3, DYNLRB1, DYNLRB2, DYNLL1, DYNLLl2). The heavy chain homodimer acts as the dynein complex core and binds microtubules to enable ATP-dependent cytoplasmic dynein movement. The other dynein subunits associate as homodimers and play regulatory roles in maintaining the complex stability, modulating its activity, and mediating its interaction with accessory and cargo proteins. The intermediate chain proteins (IC1 & IC2) are involved in cargo binding and specificity, either alone or via interaction with the dynactin complex. IC1 and IC2 share 69% protein identity. They interact with the dynein light chains and the p150 subunit of dynactin at the N-terminus and with the heavy chains through WD40 repeats at the intermediate chain C-terminus.
References
Product Information
FormatAlexaFluor®488
PresentationPurified mouse monoclonal IgG2b antibody conjugate in PBS with 15 mg/mL BSA and 0.1 % sodium azide.
Quality LevelMQ100
Applications
ApplicationAnti-Dynein Antibody, IC, cytosolic, clone 74.1, Alexa Fluor® 488 Conjugate is an antibody against Dynein for use in Immunocytochemistry.
Key Applications
  • Immunocytochemistry
Application NotesImmunocytochemistry Analysis: A 1:100 dilution from a representative lot detected cytosolic dynein intermediate chains in A431, and NIH/3T3 cells.
The unconjugated antibody (Cat. No. MAB1618) is shown to be suitable also for Western blotting, immunocytochemistry, immunofluorescence, and immunoprecipitation applications.
Biological Information
ImmunogenBovine brain cytoplasmic dynein.
EpitopeWithin N-terminal 60 amino acids.
Clone74.1
ConcentrationPlease refer to lot specific datasheet.
HostMouse
SpecificityReacts with all known forms of the 74 kDa intermediate chain (IC) subunit of mammaliam cytoplasmic dynein. The antigenic site has been mapped to the N-terminal 60 amino acids, a region conserved in intermediate chains encoded by both the DYNC1I1 and DYNC1I2 genes.
IsotypeIgG2b
Species Reactivity
  • Human
  • Xenopus
  • Fish
  • Sheep
  • Drosophila
  • Bovine
  • Rat
  • Mouse
Antibody TypeMonoclonal Antibody
Entrez Gene Number
Gene Symbol
  • DYNC1I1
  • DYNC1I2
Purification MethodProtein A Purfied
UniProt Number
Molecular Weight~74 kDa observed.
Physicochemical Information
Dimensions
Materials Information
Toxicological Information
Safety Information according to GHS
Safety Information
Product Usage Statements
Quality AssuranceEvaluated by Immunocytochemistry in HeLa cells.

Immunocytochemistry Analysis: A 1:100 dilution of this antibody detected cytosolic dynein intermediate chains in HeLa cells.
Usage Statement
  • Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
Storage and Shipping Information
Storage ConditionsStable for 1 year at 2-8°C from date of receipt.
Packaging Information
Material Size100 µL
Transport Information
Supplemental Information
Specifications
Global Trade Item Number
Catalogue Number GTIN
MAB1618-AF488 04055977333435

Documentation

Anti-Dynein Antibody, IC, cytosolic, clone 74.1, Alexa Fluor® 488 Conjugate MSDS

Title

Safety Data Sheet (SDS) 

Anti-Dynein Antibody, IC, cytosolic, clone 74.1, Alexa Fluor® 488 Conjugate Certificates of Analysis

TitleLot Number
Anti-Dynein, IC, cytosolic, clone 74.1, Alexa Fluor® 488 Conjugate - 3266601 3266601
Anti-Dynein, IC, cytosolic, clone 74.1, Alexa Fluor® 488 Conjugate - 3873555 3873555
Anti-Dynein, IC, cytosolic, clone 74.1, Alexa Fluor® 488 Conjugate -Q2667548 Q2667548