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ABC480 Anti-Dido3 Antibody

Anti-Dido3 Antibody is an antibody against Dido3 for use in Western Blotting and Immunocytochemistry.

Anti-Dido3 Antibody MSDS (material safety data sheet) or SDS, CoA and CoQ, dossiers, brochures and other available documents.
Research Category: Apoptosis & Cancer Research Sub-Category: Apoptosis - Additional Gene Symbol: DIDO1, C20orf158, DATF1, KIAA0333 UniProt Number: Q9BTC0
ABC480
100 µg  
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Key Spec Table

Species ReactivityKey ApplicationsHostFormatAntibody Type
HWB, ICCRbAffinity PurifiedPolyclonal Antibody
Description
Catalogue NumberABC480
DescriptionAnti-Dido3 Antibody
Alternate Names
  • Dido3
  • Death-inducer obliterator 1
  • DIO-1
  • hDido1
  • Death-associated transcription factor 1
  • DATF-1
Background InformationDeath inducer obliterator 3 (Dido3) is a transcription factor encoded by the DIDO1 (also known as BYE1, DIO1, DATF1, DIDO2, DIDO3) gene in human. Three other isoforms, including Dido1 & 2 (isoform 2 & 1, respectively), are also encoded by the same gene through alternative splicing. In addition to early research activities regarding apoptosis-inducing activity of Dido proteins, later studies reveal important roles of Dido1 & Dido3 (isoform 4) in maintaining stem cell genomic stability and self-renewal (PMID 23831028 & 24347171). Dido3 binds histone H3K4me3 in the nucleus. Histone H3T3 or H3T6 phosphorylation causes H3K4 demethylation and Dido3 translocation from chromatin to the mitotic spindle. Anti-Dido3, Cat. No. ABC480, does not cross-react with Dido1 or Dido2.
References
Product Information
FormatAffinity Purified
PresentationPurified rabbit polyclonal in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide.
Quality LevelMQ300
Applications
ApplicationAnti-Dido3 Antibody is an antibody against Dido3 for use in Western Blotting and Immunocytochemistry.
Key Applications
  • Western Blotting
  • Immunocytochemistry
Application NotesImmunocytochemistry Analysis: 0.5 µg/mL from a representative lot detected Dido3 in HT-29 cells.
Biological Information
ImmunogenKLH-conjugated linear peptide corresponding to human Dido3 near the C-Terminus.
EpitopeNear C-Terminus
ConcentrationPlease refer to lot specific datasheet.
HostRabbit
Species Reactivity
  • Human
Antibody TypePolyclonal Antibody
Entrez Gene Number
Gene Symbol
  • DIDO1
  • C20orf158
  • DATF1
  • KIAA0333
Purification MethodAffinity Purfied
UniProt Number
Molecular Weight~260 kDa observed. Uncharacterized band(s) may appear in some lysates.
Physicochemical Information
Dimensions
Materials Information
Toxicological Information
Safety Information according to GHS
Safety Information
Product Usage Statements
Quality AssuranceEvaluated by Western Blotting in HT-29 cell lysate.

Western Blotting Analysis: 1.0 µg/mL of this antibody detected Dido3 in 10 µg of HT-29 cell lysate.
Usage Statement
  • Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
Storage and Shipping Information
Storage ConditionsStable for 1 year at 2-8°C from date of receipt.
Packaging Information
Material Size100 µg
Transport Information
Supplemental Information
Specifications
Global Trade Item Number
Catalogue Number GTIN
ABC480 04055977158939