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MABF3126-25UG Anti-DR4/CD261 Antibody, clone DJR1

Anti-DR4/CD261, clone DJR1, Cat. No. MABF3126, is a mouse monoclonal antibody that detects DR4/CD261 and is tested for use in Flow Cytometry.

MABF3126-25UG
25 μg  
Purchase on Sigma-Aldrich

Overview

Replacement Information
Description
Catalogue NumberMABF3126-25UG
DescriptionAnti-DR4/CD261 Antibody, clone DJR1
Alternate Names
  • Tumor necrosis factor receptor superfamily member 10A
  • Death receptor 4
  • TNF-related apoptosis-inducing ligand receptor 1
  • TRAIL receptor 1
  • TRAIL-R1
Background InformationTumor necrosis factor receptor superfamily member 10A (UniProt: O00220; also known as Death receptor 4, TNF-related apoptosis-inducing ligand receptor 1, TRAIL receptor 1, TRAIL-R1, CD261) is encoded by the TNFRSF10A (also known as APO2, DR4, TRAILR1) gene (Gene ID: 8797) in human. CD261/DR4 is a single-pass type I membrane protein, which is synthesized with a signal peptide (aa 1-23) that is subsequently cleaved off to produce the mature protein that contains an extracellular domain (aa 24-239), a transmembrane domain (aa 240-262), and a cytoplasmic domain (aa 263-468). It is widely expressed with high levels observed in spleen, peripheral blood leukocytes, small intestine, and thymus. TRAIL receptors (DR4 and DR5) induce apoptosis via caspase activation. The adapter molecule FADD is reported to recruit caspase-8 to the activated receptor. The resulting death-inducing signaling complex (DISC) performs caspase-8 proteolytic activation that initiates the subsequent cascade of caspases mediating apoptosis. O-GlcNAcylation of DR4 is reported to be crucial for TRAIL death signaling. Both TRAIL-sensitive and TRAIL-resistant breast cancer cell lines can be efficiently killed by overexpression of the CD261/DR4. However, the extent of cell death depends on the strength of the promoter driving DR4 expression. (Ref.: Lee, H., et al. (2019). Cancer Res. 79; 2839-2852; Kazhdan, I., and Marciniak, RA (2004). Cancer Gene Ther. 11(10); 691-698).
References
Product Information
FormatPurified
PresentationPurified mouse monoclonal antibody IgG1 in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide.
Quality LevelMQ200
Applications
ApplicationAnti-DR4/CD261, clone DJR1, Cat. No. MABF3126, is a mouse monoclonal antibody that detects DR4/CD261 and is tested for use in Flow Cytometry.
Key Applications
  • Flow Cytometry
Application NotesTested applications

Flow Cytometry: A representative lot detected DR4/CD261 in Flow Cytometry applications (Uno, K., et al. (2003). Blood. 101(9); 3658-67; Lee, H., et al. (2019). Cancer Res. 79(11); 2839-2852).

Note: Actual optimal working dilutions must be determined by end user as specimens, and experimental conditions may vary with the end user.
Biological Information
ImmunogenRecombinant fragment corresponding to the extracellular domain of human DR4/CD261 Fc fusion protein.
EpitopeExtracellular domain
CloneDJR1
Concentration1.0 mg/mL. Please refer to guidance on suggested starting dilutions and/or titers per application and sample type.
HostMouse
SpecificityClone DJR1 is a mouse monoclonal antibody that detects DR4/CD261. It targets an epitope within the extracellular domain.
IsotypeIgG1κ
Species Reactivity
  • Human
Species Reactivity NoteHuman.
Antibody TypeMonoclonal Antibody
Entrez Gene Number
Gene Symbol
  • TNFRSF10A
Purification MethodProtein G purified
UniProt Number
Molecular Weight50.09 kDa calculated.
Physicochemical Information
Dimensions
Materials Information
Toxicological Information
Safety Information according to GHS
Safety Information
Product Usage Statements
Quality AssuranceEvaluated by Flow Cytometry in BHK cells transfected with Human DR4.

Flow Cytometry Analysis: 1.0 µg of this antibody detected DR4/CD261 in BHK cells transfected with Human DR4.
Usage Statement
  • Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
Storage and Shipping Information
Storage ConditionsRecommended storage: +2°C to +8°C.
Packaging Information
Material Size25 μg
Transport Information
Supplemental Information
Specifications
Global Trade Item Number
Catalogue Number GTIN
MABF3126-25UG 04065269802518