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ABE1327-25UG Anti-DPF2

Detect BRG1-associated factor 45D using this rabbit polyclonal Anti-DPF2, Cat. No. ABE1327-25UG, validated for use in Western Blotting and Immunocytochemistry.

Anti-DPF2 MSDS (material safety data sheet) or SDS, CoA and CoQ, dossiers, brochures and other available documents.
Research Category: Apoptosis & Cancer Gene Symbol: DPF2, BAF45D, REQ, UBID4 UniProt Number: Q92785
ABE1327-25UG
25 μg  
Purchase on Sigma-Aldrich

Overview

Replacement Information

Key Spec Table

Species ReactivityKey ApplicationsHostFormatAntibody Type
H, R, MWB, ICCRbPurifiedPolyclonal Antibody
Description
Catalogue NumberABE1327-25UG
DescriptionAnti-DPF2
Alternate Names
  • Zinc finger protein ubi-d4
  • Apoptosis response zinc finger protein
  • BRG1-associated factor 45D
  • BAF45D
  • D4 zinc and double PHD fingers family 2
  • Protein requiem
Background InformationZinc finger protein ubi-d4 (UniProt: Q92785; also known as Apoptosis response zinc finger protein, BRG1-associated factor 45D, BAF45D, D4, zinc and double PHD fingers family 2, Protein requiem, DFP2) is encoded by the DPF2 (also known as BAF45D, REQ, UBID4) gene (Gene ID: 5977) in human. BAF45D is a member of the requiem/DPF protein family and is ubiquitously expressed. It may serve as a transcription factor required for the apoptosis response following survival factor withdrawal from myeloid cells. It is also believed to have a role in the development and maturation of lymphoid cells. It contains 3 zinc-finger domains (C2H2-type aa 209-232; PHD type I aa 270-330; and PHD type 2 aa 327-377). Two isoforms of BAF45D have been described that are generated via alternative splicing.
References
Product Information
FormatPurified
PresentationPurified rabbit polyclonal antibody in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide.
Quality LevelMQ100
Applications
ApplicationDetect BRG1-associated factor 45D using this rabbit polyclonal Anti-DPF2, Cat. No. ABE1327-25UG, validated for use in Western Blotting and Immunocytochemistry.
Key Applications
  • Western Blotting
  • Immunocytochemistry
Application NotesWestern Blotting Analysis: 2 µg/mL from a representative lot detected DPF2 in 10 µg of  PC12 cell lysate.
Immunocytochemistry Analysis: A 1:100-500 dilution from a representative lot detected DPF2 in A431 and HepG2 cells.
Biological Information
ImmunogenKLH-conjugated liner peptide that corresponding to 17 amino acids from the N-terminal region of human BRG1-associated factor 45D (DPF2).
ConcentrationPlease refer to lot specific datasheet.
HostRabbit
SpecificityThis rabbit polyclonal antibody detects BRG1-associated factor 45D in human, mouse, and rat species. It targets an epitope within 17 amino acids from the N-terminal half. The immunogen sequence is conserved in both isoforms.
IsotypeIgG
Species Reactivity
  • Human
  • Rat
  • Mouse
Species Reactivity NoteHuman, Mouse, Rat.
Antibody TypePolyclonal Antibody
Gene Symbol
  • DPF2
  • BAF45D
  • REQ
  • UBID4
Purification MethodAffinity Purified
UniProt Number
Molecular Weight44 kDa observed. Uncharacterized bands may be observed in some lysate(s).
Physicochemical Information
Dimensions
Materials Information
Toxicological Information
Safety Information according to GHS
Safety Information
Product Usage Statements
Quality AssuranceEvaluated by Western Blotting in NIH3T3 cell lysate.
Western Blotting Analysis: 2 µg/mL of this antibody detected DPF2 in 10 µg of NIH3T3 cell lysate.
Usage Statement
  • Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
Storage and Shipping Information
Storage ConditionsStable for 1 year at 2-8°C from date of receipt.
Packaging Information
Material Size25 μg
Transport Information
Supplemental Information
Specifications
Global Trade Item Number
Catalogue Number GTIN
ABE1327-25UG 04054839453786