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MAB3232 Anti-Cytokeratin 14 Antibody, clone RCK107

MAB3232
100 µg  
Purchase on Sigma-Aldrich

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Overview

Key Spec Table

Species ReactivityKey ApplicationsHostFormatAntibody Type
Ca, H, Po, RFC, WB, ICC, IHCMPurifiedMonoclonal Antibody
Description
Catalogue NumberMAB3232
Brand Family Chemicon®
Trade Name
  • Chemicon
DescriptionAnti-Cytokeratin 14 Antibody, clone RCK107
Background InformationCytokeratins are a subfamily of intermediate filament proteins and are characterized by a remarkable biochemical diversity, represented in epithelial tissues by at least 20 different polypeptides. They range in molecular weight from between 40 kDa and 68 kDa and isoelectric pH between 4.9 - 7.8, The individual cytokeratin polypeptides are designated 1-20. The various epithelia in the human body usually express cytokeratins which are not only characteristic of the type of epithelium, but also related to the degree of maturation or differentiation within an epithelium. Cytokeratin subtype expression patterns are used to an increasing extent in the distinction of different types of epithelial malignancies.
Product Information
FormatPurified
PresentationLiquid in buffer with 0.1% sodium azide.
Quality LevelMQ100
Applications
ApplicationDetect Cytokeratin 14 using this Anti-Cytokeratin 14 Antibody, clone RCK107 validated for use in FC, WB, IC, IH.
Key Applications
  • Flow Cytometry
  • Western Blotting
  • Immunocytochemistry
  • Immunohistochemistry
Application NotesWestern blot

Immunohistochemistry on frozen tissue sections.

Immunocytochemistry

Flow cytometry

Optimal working dilutions must be determined by the end user.
Biological Information
ImmunogenCytoskeletal preparation of TR146 epithelial cells.
CloneRCK107
ConcentrationPlease refer to the Certificate of Analysis for the lot-specific concentration.
HostMouse
SpecificityCytokeratin 14. MAB3232 reacts with basal cell compartments of stratified and combined epithelia.Reacts with cytokeratin 14, expressed in the basal cells of the larynx, esophagus, trachea, bladder, prostate, cervix, vagina, breast acini, skin and sweat glands.
IsotypeIgG3
Species Reactivity
  • Canine
  • Human
  • Pig
  • Rat
Antibody TypeMonoclonal Antibody
Entrez Gene Number
Entrez Gene SummaryThis gene encodes a member of the keratin family, the most diverse group of intermediate filaments. This gene product, a type I keratin, is usually found as a heterotetramer with two keratin 5 molecules, a type II keratin. Together they form the cytoskeleton of epithelial cells. Mutations in the genes for these keratins are associated with epidermolysis bullosa simplex. At least one pseudogene has been identified at 17p12-p11.
Gene Symbol
  • KRT14
  • Cytokeratin-14
  • NFJ
  • CK-14
  • CK14
  • EBS3
  • EBS4
  • K14
  • Keratin-14
UniProt Number
UniProt SummarySIZE: 472 amino acids; 51622 Da
SUBUNIT: Heterotetramer of two type I and two type II keratins. keratin-14 associates with keratin-5. Interacts with TRADD.
TISSUE SPECIFICITY: Detected in the basal layer, lowered within the more apically located layers specifically in the stratum spinosum, stratum granulosum but is not detected in stratum corneum. Strongly expressed in the outer root sheath of anagen follicles but not in the germinative matrix, inner root sheath or hair. Found in keratinocytes surrounding the club hair during telogen.
DISEASE: SwissProt: P02533 # Defects in KRT14 are a cause of epidermolysis bullosa simplex (EBS) [MIM:131760, 131800, 131900, 601001]. Epidermolysis bullosa (EB) is a mendelian disorder that can be inherited in an autosomal dominant or recessive form and is characterized by blistering or erosion of the skin and mucous membranes as a result of mild trauma. The incidence at birth of all types of the disease is about 1:50,000. Three major clinical subgroups are: simplex (EBS), junctional (EBJ), and dystrophic (EBD). EBS is by far the most prevalent (65%). Blistering occurs within the basal cell layer and is further divided into three subgroups. Junctional and dystrophic EB are characterized by blistering in the plane of the lamina lucida of the basement membrane zone and below the dermal- epidermal basement membrane at the level of the anchoring fibrils, respectively. & Defects in KRT14 are a cause of epidermolysis bullosa simplex Dowling-Meara type (DM-EBS) [MIM:131760]. DM-EBS is the most severe of the simplex group and is characterized by generalized herpetiform blistering, milia formation, dystrophic nails, and mucous membrane involvement. & Defects in KRT14 are a cause of epidermolysis bullosa simplex Weber-Cockayne type (WC-EBS) [MIM:131800]. WC-EBS is characterized by blistering in palmar and plantar areas of the skin. & Defects in KRT14 are a cause of epidermolysis bullosa simplex Koebner type (K-EBS) [MIM:131900]. K-EBS is characterized by generalized blistering as the Dowling-Meara type; the phenotype however is less severe. & Defects in KRT14 are the cause of Naegeli-Franceschetti- Jadassohn syndrome (NFJS) [MIM:161000]; also known as Naegeli syndrome. NFJS is a rare autosomal dominant form of ectodermal dysplasia. The cardinal features are absence of dermatoglyphics (fingerprints), reticular cutaneous hyperpigmentation (starting at about the age of 2 years without a preceding inflammatory stage), palmoplantar keratoderma, hypohidrosis with diminished sweat gland function and discomfort provoked by heat, nail dystrophy, and tooth enamel defects. & Defects in KRT14 are the cause of dermatopathia pigmentosa reticularis (DPR) [MIM:125595]. DPR is a rare ectodermal dysplasia characterized by lifelong persistant reticulate hyperpigmentation, noncicatricial alopecia, and nail dystrophy.
SIMILARITY: SwissProt: P02533 ## Belongs to the intermediate filament family.
MISCELLANEOUS: There are two types of cytoskeletal and microfibrillar keratin: I (acidic; 40-55 kDa) and II (neutral to basic; 56-70 kDa).
Product Usage Statements
Usage Statement
  • Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
Storage and Shipping Information
Storage ConditionsMaintain at -20°C in undiluted aliquots up to 6 months. Avoid repeated freeze/thaw cycles.
Packaging Information
Material Size100 µg
Global Trade Item Number
Catalogue Number GTIN
MAB3232 04053252314667