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MABE644 Anti-Cre Recombinase Antibody, clone 5B4.1

Anti-Cre Recombinase Antibody, clone 5B4.1, Cat. No. MABE644, is a highly specific mouse monoclonal antibody that targets Cre recombinase and has been tested in Western Blotting.

Anti-Cre Recombinase Antibody, clone 5B4.1 MSDS (material safety data sheet) or SDS, CoA and CoQ, dossiers, brochures and other available documents.
Research Category: Epigenetics & Nuclear Function Gene Symbol: cre UniProt Number: P06956
MABE644
100 µL  
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Key Spec Table

Species ReactivityKey ApplicationsHostFormatAntibody Type
AWBMPurifiedMonoclonal Antibody
Description
Catalogue NumberMABE644
ReplacesMABE3120
DescriptionAnti-Cre Recombinase Antibody, clone 5B4.1
Alternate Names
  • Recombinase cre
Background InformationRecombinase cre (UniProt P06956) is encoded by the cre gene (Gene ID 2777477) of bacteriophage P1. Cre (Causes recombination with Cyclization recombinase) is a 38 kDa DNA recombinase derived from the P1 bacteriophage. It is highly specific for a 34-bp DNA sequence motif called loxP (locus of X-over P1) found in P1 DNA. The loxP site is composed of a variable asymmetric 8-bp sequence in between two sets of 13-bp palindromic sequences (ATAACTTCGTATA-NNNTANNN-TATACGAAGTTAT). Two cre recombinase proteins, each binds to one of the two 13-bp palindromic sequences at a loxP site, form a dimer. This dimer then binds to a second dimer on another loxP site to form a tetramer. The two sites joined by the tetramer are parallel in orientation and the double stranded DNA is cut at both loxP sites. The strands are then rejoined with DNA ligase. Because the Cre gene and loxP sites are not native to most species, they are often introduced into target cells or tissues and employed as a means to precisely control the expression of genes both in cultured cells in vitro and in animals vivo.
References
Product Information
FormatPurified
PresentationPurified mouse IgG1 in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide.
Quality LevelMQ100
Applications
ApplicationAnti-Cre Recombinase Antibody, clone 5B4.1, Cat. No. MABE644, is a highly specific mouse monoclonal antibody that targets Cre recombinase and has been tested in Western Blotting.
Key Applications
  • Western Blotting
Biological Information
ImmunogenGST-tagged recombinant Cre lacking the N-terminal region sequence.
Clone5B4.1
ConcentrationPlease refer to lot specific datasheet.
HostMouse
SpecificityClone 5B4.1 detects an epitope outside the N-terminal region.
IsotypeIgG1κ
Species Reactivity
  • All
Species Reactivity NoteVirus
Antibody TypeMonoclonal Antibody
Entrez Gene Number
Gene Symbol
  • cre
Purification MethodProtein G purified.
UniProt Number
Molecular Weight38.54 kDa calculated.
Physicochemical Information
Dimensions
Materials Information
Toxicological Information
Safety Information according to GHS
Safety Information
Product Usage Statements
Quality AssuranceEvaluated by Western Blotting in recombinant Cre.

Western Blotting Analysis: A 1:2,000 dilution of this antibody detected 250 ng of recombinant Cre (with added N- and C-terminal tag sequences).
Usage Statement
  • Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
Storage and Shipping Information
Storage ConditionsStable for 1 year at 2-8°C from date of receipt.
Packaging Information
Material Size100 µL
Transport Information
Supplemental Information
Specifications
Global Trade Item Number
Catalogue Number GTIN
MABE644 04054839086113