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CP45 Anti-Clathrin Heavy Chain Mouse mAb (X22)

This Anti-Clathrin Heavy Chain Mouse mAb (X22) is validated for use in Immunoblotting, Immunofluorescence, Immunoprecipitation for the detection of Clathrin Heavy Chain.

Anti-Clathrin Heavy Chain Mouse mAb (X22) MSDS (material safety data sheet) or SDS, CoA and CoQ, dossiers, brochures and other available documents.
CP45
Purchase on Sigma-Aldrich

Overview

Replacement Information

Key Spec Table

Species ReactivityHostAntibody Type
B, H, M, RMMonoclonal Antibody

Products

Catalogue NumberPackaging Qty/Pack
CP45-100UL Plastová ampulka 100 ul
Description
OverviewRecognizes the ~180 kDa clathrin heavy chain protein.
Catalogue NumberCP45
Brand Family Calbiochem®
References
ReferencesBauerfeind, R., et al. 1996. J. Neurocytol. 25, 701.
Zhang, J.Z., et al. 1994. Cell 78, 751.
Herskovits, J.S., et al. 1993. J. Cell Biol. 122, 565.
Van Der Bliek, A.M., et al. 1993. J. Cell Biol. 122, 553.
Pearse, B.M. and Robinson, M.S. 1990. Ann. Rev. Cell Biol. 6, 151.
Brodsky, F.M. 1985. J. Cell Biol. 101, 2047.
Product Information
FormLiquid
FormulationIn PBS.
Preservative≤0.1% sodium azide
Quality LevelMQ100
Applications
Key Applications Immunoblotting (Western Blotting)
Immunofluorescence
Immunoprecipitation
Application NotesImmunoblotting (1:500)
Immunofluorescence (1:1000, indirect)
Immunoprecipitation (see comments)
Application CommentsImmunoprecipitation of triskelions allows both the clathrin heavy chain and the associated light chain polypeptides to be examined. Antibody should be titrated for optimal results in individual systems.
Biological Information
Immunogenclathrin heavy chain purified from human brain
ImmunogenHuman
CloneX22
HostMouse
IsotypeIgG₁
Species Reactivity
  • Bovine
  • Human
  • Mouse
  • Rat
Antibody TypeMonoclonal Antibody
Concentration Label Please refer to vial label for lot-specific concentration
Physicochemical Information
Dimensions
Materials Information
Toxicological Information
Safety Information according to GHS
Safety Information
Product Usage Statements
Storage and Shipping Information
Ship Code Dry Ice Only
Toxicity Standard Handling
Storage -20°C
Avoid freeze/thaw Avoid freeze/thaw
Do not freeze Ok to freeze
Special InstructionsFollowing initial thaw, aliquot and freeze (-20°C).
Packaging Information
Transport Information
Supplemental Information
Specifications
Global Trade Item Number
Catalogue Number GTIN
CP45-100UL 04055977220995

Documentation

Anti-Clathrin Heavy Chain Mouse mAb (X22) MSDS

Title

Safety Data Sheet (SDS) 

Anti-Clathrin Heavy Chain Mouse mAb (X22) Certificates of Analysis

TitleLot Number
CP45

References

Reference overview
Bauerfeind, R., et al. 1996. J. Neurocytol. 25, 701.
Zhang, J.Z., et al. 1994. Cell 78, 751.
Herskovits, J.S., et al. 1993. J. Cell Biol. 122, 565.
Van Der Bliek, A.M., et al. 1993. J. Cell Biol. 122, 553.
Pearse, B.M. and Robinson, M.S. 1990. Ann. Rev. Cell Biol. 6, 151.
Brodsky, F.M. 1985. J. Cell Biol. 101, 2047.
Data Sheet

Note that this data sheet is not lot-specific and is representative of the current specifications for this product. Please consult the vial label and the certificate of analysis for information on specific lots. Also note that shipping conditions may differ from storage conditions.

Revision22-August-2007 RFH
ApplicationImmunoblotting (1:500)
Immunofluorescence (1:1000, indirect)
Immunoprecipitation (see comments)
DescriptionPurified mouse monoclonal antibody generated by immunizing BALB/c mice with the specified immunogen and fusing splenocytes with Sp2/0-Ag14 mouse myeloma cells. Recognizes the ~180 kDa clathrin heavy chain protein.
BackgroundAt the unstimulated presynaptic nerve terminal, a pool of synaptic vesicles remains docked at active zones in preparation for exocytosis. Nerve terminal depolarization induced through an action potential stimulates the rise in intracellular Ca2+, which ultimately initiates the exocytotic process. Fused synaptic vesicles are rapidly replaced with vesicles from the reserve pool, while membranes of the fused vesicles are recaptured by endocytosis. Clathrin is one component involved in vesicle endocytosis, and is composed of three polypeptides, a ~180 kDa heavy chain and two ~32-38 kDa light chains which combine to create a distinct three-legged triskelion. It is this morphology that allows clathrin to form its unique polyhedral network. The clathrin-adaptor AP2 and the AP180/AP3 proteins also play a role in this process by forming a protein layer between the membrane and clathrin lattice. The interaction of the AP2 heterotetramer with synaptotagmin is believed to act as the template for recruitment of the AP3 and clathrin triskelia complex. Progression from clathrin-coated to free vesicles in the nerve terminus also requires dynamin.
HostMouse
Immunogen speciesHuman
Immunogenclathrin heavy chain purified from human brain
CloneX22
IsotypeIgG₁
Speciesbovine, human, mouse, rat
FormLiquid
FormulationIn PBS.
Concentration Label Please refer to vial label for lot-specific concentration
Preservative≤0.1% sodium azide
CommentsImmunoprecipitation of triskelions allows both the clathrin heavy chain and the associated light chain polypeptides to be examined. Antibody should be titrated for optimal results in individual systems.
Storage Avoid freeze/thaw
-20°C
Do Not Freeze Ok to freeze
Special InstructionsFollowing initial thaw, aliquot and freeze (-20°C).
Toxicity Standard Handling
ReferencesBauerfeind, R., et al. 1996. J. Neurocytol. 25, 701.
Zhang, J.Z., et al. 1994. Cell 78, 751.
Herskovits, J.S., et al. 1993. J. Cell Biol. 122, 565.
Van Der Bliek, A.M., et al. 1993. J. Cell Biol. 122, 553.
Pearse, B.M. and Robinson, M.S. 1990. Ann. Rev. Cell Biol. 6, 151.
Brodsky, F.M. 1985. J. Cell Biol. 101, 2047.