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MABF3035-100UL Anti-Chikungunya virus E1 Antibody, clone CHK-166

Anti-Chikungunya virus E1, clone CHK-166, Cat. No. MABF3035, is a mouse monoclonal antibody that detects Chikungunya virus (CHIKV) E1 protein and is tested for use in ELISA, Inhibition, and Neutralizing assay.

MABF3035-100UL
100 µL  
Purchase on Sigma-Aldrich

Overview

Replacement Information
Description
Catalogue NumberMABF3035-100UL
DescriptionAnti-Chikungunya virus E1 Antibody, clone CHK-166
Alternate Names
  • Spike glycoprotein E1
  • Chikungunya virus E1 Protein
Background InformationChikungunya virus (CHIKV) that causes distinctive polyarthritis or polyarthralgia with clinical features such as fever, maculopapular rash, and myalgia is transmitted by Aedes spp of mosquitoes. Immune compromised individuals may face serious complications, including encephalitis and mortality. CHIKV genome contains a single-stranded positive-sense RNA that encodes four non-structural proteins known as nsP1, nsP2, nsP3, and nsP4 and also five structural proteins that include a capsid protein, three envelope glycoproteins known as (E1, E2, and E3, and a small molecule known as 6K. The mature alphavirus particles express E1 and E2 heterodimers that form 80 trimeric spikes on the surface of the virion. The mature E2 protein contains three domains with immunoglobulin-like folds: N-terminal domain A, domain B at the tip; and the C-terminal domain C located proximal to the viral membrane. The ectodomain E1 protein consists of three domains known as D1, DII, and DIII. The DIII domain is an immunoglobulin-like domain connected to D1 and DII by a flexible linker. Both E1 and E2 proteins are responsible for virus entry into host cells. The E2 glycoprotein interacts with a cellular receptor, resulting in the virus internalization and the E1 glycoprotein mediates virus fusion to host cell under low pH conditions. Following the fusion of the viral envelope with the endosomal membrane, the viral genomic RNA is released into the cytoplasm and starts replicating. Clone CHK-166 is a moderately inhibitory antibody that protects Ifr -/- mice from lethal infection and when used with clone CHK-152 it provides viral resistance and extends the treatment window by up to 36 hours post infection. (Ref.: Zhang, R., et al. (2019). Cell Reports 28(10); 2647-2658; Pal, P., et al. (2013). PLoS Pathog. 9(4); e1003312).
References
Product Information
FormatPurified
PresentationPurified mouse monoclonal antibody IgG2a in PBS without azide.
Quality LevelMQ200
Applications
ApplicationAnti-Chikungunya virus E1, clone CHK-166, Cat. No. MABF3035, is a mouse monoclonal antibody that detects Chikungunya virus (CHIKV) E1 protein and is tested for use in ELISA, Inhibition, and Neutralizing assay.
Key Applications
  • ELISA
  • Inhibition
  • Neutralizing
Application NotesTested Applications

Inhibition Analysis: A representative lot inhibited CHIKV infection when added after virus adsorption to the cell surface. (Pal, P., et al. (2013). PLoS Pathog. 9(4):e1003312).

ELISA Analysis: A representative lot detected Chikungunya virus E1 in ELISA applications (Zhang, R., et al. (2019). Cell Rep. 28(10): 2647-2658.e5).

Neutralizing Analysis: A representative lot neutralized CHIKV and protected immunocompromised mice against disease. (Pal, P., et al. (2013). PLoS Pathog. 9(4):e1003312).

Note: Actual optimal working dilutions must be determined by end user as specimens, and experimental conditions may vary with the end user
Biological Information
ImmunogenIrf-7 deficient mice injected with La Reunion 2006 OPY-1 strain of Chikungunya virus (CHIKV-LR) and boosted with CHIKV virus-like particles.
EpitopeN-terminal
CloneCHK-166
Concentration0.5 mg/mL. Please refer to guidance on suggested starting dilutions and/or titers per application and sample type.
HostMouse
SpecificityClone CHK-166 is a mouse monoclonal antibody that detects E1 protein of Chikungunya virus. It targets an epitope within the DII region of E1 protein, adjacent to the fusion loop.
IsotypeIgG2aκ
Species Reactivity
  • Virus
Species Reactivity NoteChikungunya virus.
Antibody TypeMonoclonal Antibody
Purification MethodProtein G purified
UniProt Number
Molecular Weight138.07 kDa for Structural polyprotein.
Physicochemical Information
Dimensions
Materials Information
Toxicological Information
Safety Information according to GHS
Safety Information
Product Usage Statements
Quality AssuranceEvaluated by ELISA with recombinant Chikungunya Virus E1 protein.

ELISA Analysis: Various dilutions of this antibody detected recombinant Chikungunya virus E1 protein.
Usage Statement
  • Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
Storage and Shipping Information
Storage ConditionsStore at -10°C to -25°C. Handling Recommendations: Upon receipt and prior to removing the cap, centrifuge the vial and gently mix the solution. Aliquot into microcentrifuge tubes and store at -20°C. Avoid repeated freeze/thaw cycles, which may damage IgG and affect product performance.
Packaging Information
Material Size100 µL
Transport Information
Supplemental Information
Specifications
Global Trade Item Number
Catalogue Number GTIN
MABF3035-100UL 04065267027586

Documentation

Anti-Chikungunya virus E1 Antibody, clone CHK-166 MSDS

Title

Safety Data Sheet (SDS) 

Anti-Chikungunya virus E1 Antibody, clone CHK-166 Certificates of Analysis

TitleLot Number
Anti-Chikungunya virus E1, clone CHK-166 - 4080520 4080520
Anti-Chikungunya virus E1, clone CHK-166 - 4089928 4089928
Anti-Chikungunya virus E1, clone CHK-166 - 4090972 4090972
Anti-Chikungunya virus E1, clone CHK-166 - 4093608 4093608
Anti-Chikungunya virus E1, clone CHK-166 - 4116106 4116106
Anti-Chikungunya virus E1, clone CHK-166 - Q3758869 Q3758869