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PC25 Anti-Cdc2/Cdk1 (Ab-1) Rabbit pAb

Anti-Cdc2/Cdk1 (Ab-1) Rabbit pAb MSDS (material safety data sheet) or SDS, CoA and CoQ, dossiers, brochures and other available documents.

Synonyms: Anti-Cdk1, Anti-p34

PC25
  
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Overview

Replacement Information

Key Spec Table

Host
Rb
Description
Overview

This product has been discontinued.





Recognizes the ~34 kDa Cdc2/Cdk1 protein in proliferating HeLa cells.
Catalogue NumberPC25
Brand Family Calbiochem®
SynonymsAnti-Cdk1, Anti-p34
Application Data
References
ReferencesReed, S.I., and Wittenberg, C. 1990. Proc. Natl. Acad. Sci. USA 87, 5697.
Draetta, G., et al. 1987. Cell 50, 319.
Simanis, V., and Nurse, P. 1986. Cell 45, 261.
Reed, S.I., et al. 1985. Proc. Natl. Acad. Sci. USA 82, 4055.
Piggott, J.R., et al. 1982. Nature 298, 391.
Nurse, P., and Bisset, Y. 1981. Nature 292, 558.
Product Information
FormLiquid
FormulationIn PBS.
Positive controlProliferating human or rodent cells
Preservative≤0.1% sodium azide
Applications
Key Applications Immunoblotting (Western Blotting)
Immunofluorescence
Immunoprecipitation
Application NotesImmunoblotting (1-5 µg/ml)
Immunofluorescence (1-5 µg/ml)
Immunoprecipitation (1-2 µg/sample)
Application CommentsImmunoprecipitates the active kinase. Does not react with most Cdc2 related proteins. Antibody should be titrated for optimal results in individual sample types.
Biological Information
Immunogena synthetic peptide (CDNQIKKM) corresponding to the C-terminus of human Cdc2/Cdk1
ImmunogenHuman
HostRabbit
IsotypeIgG
Concentration Label Please refer to vial label for lot-specific concentration
Physicochemical Information
Dimensions
Materials Information
Toxicological Information
Safety Information according to GHS
Safety Information
Product Usage Statements
Storage and Shipping Information
Ship Code Blue Ice Only
Toxicity Standard Handling
Storage -20°C
Do not freeze Ok to freeze
Packaging Information
Transport Information
Supplemental Information
Specifications
Global Trade Item Number
Catalogue Number GTIN
PC25 0

Documentation

Anti-Cdc2/Cdk1 (Ab-1) Rabbit pAb MSDS

Title

Safety Data Sheet (SDS) 

Anti-Cdc2/Cdk1 (Ab-1) Rabbit pAb Certificates of Analysis

TitleLot Number
PC25

References

Reference overview
Reed, S.I., and Wittenberg, C. 1990. Proc. Natl. Acad. Sci. USA 87, 5697.
Draetta, G., et al. 1987. Cell 50, 319.
Simanis, V., and Nurse, P. 1986. Cell 45, 261.
Reed, S.I., et al. 1985. Proc. Natl. Acad. Sci. USA 82, 4055.
Piggott, J.R., et al. 1982. Nature 298, 391.
Nurse, P., and Bisset, Y. 1981. Nature 292, 558.
Data Sheet

Note that this data sheet is not lot-specific and is representative of the current specifications for this product. Please consult the vial label and the certificate of analysis for information on specific lots. Also note that shipping conditions may differ from storage conditions.

Revision01-July-2008 JSW
SynonymsAnti-Cdk1, Anti-p34
ApplicationImmunoblotting (1-5 µg/ml)
Immunofluorescence (1-5 µg/ml)
Immunoprecipitation (1-2 µg/sample)
Application Data
DescriptionPurified rabbit polyclonal antibody. Recognizes the ~34 kDa Cdc2/Cdk1 protein.
BackgroundThe cdc2 gene was originally isolated using cell division cycle mutants in the fission yeast Schizosaccharomyces pombe. The gene was shown to encode a protein with a molecular weight of ~34 kDa and had all of the properties of protein kinase. The cdc2 gene was subsequently shown to be functionally homologous to the Cdc28 gene in the budding yeast, Saccharomyces cerevisiae, which was demonstrated to encode a protein (serine/threonine) kinase. The Cdc2 kinase is now known to be a key regulator of the eukaryotic cell cycle and is believed to act in both G1 and G2 phases. The activity of the catalytic subunit p34 is controlled by interactions with other proteins, namely the mitotic cyclins and p13suc1, and by phosphorylation-dephosphorylation. The kinase activity of dc2 is most readily studied by using histone H1 as a phosphorylation substrate in vitro. cdc2 is also referred to as cdk1.
HostRabbit
Immunogen speciesHuman
Immunogena synthetic peptide (CDNQIKKM) corresponding to the C-terminus of human Cdc2/Cdk1
IsotypeIgG
Specieshuman, mouse, rat
Positive controlProliferating human or rodent cells
FormLiquid
FormulationIn PBS.
Concentration Label Please refer to vial label for lot-specific concentration
Preservative≤0.1% sodium azide
CommentsImmunoprecipitates the active kinase. Does not react with most Cdc2 related proteins. Antibody should be titrated for optimal results in individual sample types.
Storage -20°C
Do Not Freeze Ok to freeze
Toxicity Standard Handling
ReferencesReed, S.I., and Wittenberg, C. 1990. Proc. Natl. Acad. Sci. USA 87, 5697.
Draetta, G., et al. 1987. Cell 50, 319.
Simanis, V., and Nurse, P. 1986. Cell 45, 261.
Reed, S.I., et al. 1985. Proc. Natl. Acad. Sci. USA 82, 4055.
Piggott, J.R., et al. 1982. Nature 298, 391.
Nurse, P., and Bisset, Y. 1981. Nature 292, 558.