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IM16 Anti-Cathepsin D (Ab-2) Rabbit pAb

Anti-Cathepsin D (Ab-2) Rabbit pAb MSDS (material safety data sheet) or SDS, CoA and CoQ, dossiers, brochures and other available documents.
IM16
  
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Overview

Replacement Information

Key Spec Table

Species ReactivityHostAntibody Type
HRbPolyclonal Antibody
Description
Overview

This product has been discontinued.



Recognizes the ~52 kDa latent form D and the ~34 kDa subunit, but not the ~14 kDa subunit of cathepsin D in breast carcinoma tissue extracts.
Catalogue NumberIM16
Brand Family Calbiochem®
References
ReferencesRochefort, H. 1992. Eur. J. Cancer 28A, 1780.
Rochefort, H. 1990. In Seminars in Cancer Biology, ed. M. M. Gottesman. Vol. 1(2), 153.
Westly, B. and Rochefort, H. 1980. Cell 20, 352.
Barret, A. J. 1977. In Proteinases in Mammalian Cells and Tissues, ed. Barret, A. J., North Holland, New York, p 209.
Product Information
FormLiquid
FormulationIn 50 mM sodium phosphate buffer, 0.2% gelatin, pH 7.5.
Positive controlHT1080 concentrated conditioned medium or breast carcinoma tissue
Preservative≤0.1% sodium azide
Quality LevelMQ100
Applications
Key Applications Frozen Sections
Immunoblotting (Western Blotting)
Paraffin Sections
Application NotesFrozen Sections (5 µg/ml)
Immunoblotting (2.5 µg/ml)
Paraffin Sections (5 µg/ml, trypsin pre-treatment required)
Application CommentsFor optimal results in immunoblotting, conditioned media from HT1080 cells should be concentrated 10-20X prior to loading. Antibody should be titrated for optimal results in individual systems.
Biological Information
Immunogenpurified human liver cathepsin D
ImmunogenHuman
HostRabbit
IsotypeIgG
Species Reactivity
  • Human
Antibody TypePolyclonal Antibody
Concentration Label Please refer to vial label for lot-specific concentration
Physicochemical Information
Dimensions
Materials Information
Toxicological Information
Safety Information according to GHS
Safety Information
Product Usage Statements
Storage and Shipping Information
Ship Code Blue Ice Only
Toxicity Standard Handling
Storage +2°C to +8°C
Do not freeze Yes
Packaging Information
Transport Information
Supplemental Information
Specifications
Global Trade Item Number
Catalogue Number GTIN
IM16 0

Documentation

Anti-Cathepsin D (Ab-2) Rabbit pAb MSDS

Title

Safety Data Sheet (SDS) 

Anti-Cathepsin D (Ab-2) Rabbit pAb Certificates of Analysis

TitleLot Number
IM16

References

Reference overview
Rochefort, H. 1992. Eur. J. Cancer 28A, 1780.
Rochefort, H. 1990. In Seminars in Cancer Biology, ed. M. M. Gottesman. Vol. 1(2), 153.
Westly, B. and Rochefort, H. 1980. Cell 20, 352.
Barret, A. J. 1977. In Proteinases in Mammalian Cells and Tissues, ed. Barret, A. J., North Holland, New York, p 209.

Brochure

Title
Cathepsins and Related Products Technical Bulletin
Data Sheet

Note that this data sheet is not lot-specific and is representative of the current specifications for this product. Please consult the vial label and the certificate of analysis for information on specific lots. Also note that shipping conditions may differ from storage conditions.

Revision27-August-2007 RFH
ApplicationFrozen Sections (5 µg/ml)
Immunoblotting (2.5 µg/ml)
Paraffin Sections (5 µg/ml, trypsin pre-treatment required)
DescriptionPurified rabbit polyclonal antibody. Recognizes the ~52 kDa latent form and the ~34 kDa (but not the ~14 kDa) active subunit of cathepsin D.
BackgroundCathepsin D (CD) is a normal lysosomal proteinase expressed in all cells. It is an aspartyl proteinase, with a pH optimum in the range of 3-5, and contains two N-linked oligosaccharides. CD is synthesized as an inactive 52 kDa pro-enzyme. Activation involves the proteolytic removal of the 43 amino acid pro fragment and an internal cleavage to generate the two chain form made up of 34 and 14 kDa subunits. It contains the mannose-6-phosphate lysosomal localization signal which targets the enzyme to the lysosomal compartment where it functions in the normal degradation of proteins. In certain tumor cells, CD is abnormally processed and is secreted in its 52 kDa precursor form. Numerous clinical studies as well as in vitro evidence suggest that CD plays an important role in malignant transformation and may be a useful prognostic indicator for breast cancer.
HostRabbit
Immunogen speciesHuman
Immunogenpurified human liver cathepsin D
IsotypeIgG
Specieshuman
Positive controlHT1080 concentrated conditioned medium or breast carcinoma tissue
FormLiquid
FormulationIn 50 mM sodium phosphate buffer, 0.2% gelatin, pH 7.5.
Concentration Label Please refer to vial label for lot-specific concentration
Preservative≤0.1% sodium azide
CommentsFor optimal results in immunoblotting, conditioned media from HT1080 cells should be concentrated 10-20X prior to loading. Antibody should be titrated for optimal results in individual systems.
Storage +2°C to +8°C
Do Not Freeze Yes
Toxicity Standard Handling
ReferencesRochefort, H. 1992. Eur. J. Cancer 28A, 1780.
Rochefort, H. 1990. In Seminars in Cancer Biology, ed. M. M. Gottesman. Vol. 1(2), 153.
Westly, B. and Rochefort, H. 1980. Cell 20, 352.
Barret, A. J. 1977. In Proteinases in Mammalian Cells and Tissues, ed. Barret, A. J., North Holland, New York, p 209.