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AP1043 Anti-Caspase-1 Mouse mAb (14F468)

Anti-Caspase-1 Mouse mAb (14F468) MSDS (material safety data sheet) or SDS, CoA and CoQ, dossiers, brochures and other available documents.

Synonyms: Anti-CASP1, Anti-Interleukin-1β convertase, Anti-IL-1BC, Anti-IL-1β-converting enzyme, Anti-ICE, Anti-Interleukin-1β-converting enzyme

AP1043
  
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Overview

Replacement Information

Key Spec Table

Host
M
Description
Overview

This product has been discontinued.





Recognizes the ~45 kDa latent form of caspase-1 in HeLa and NIH3T3 cells. Also recognizes cleaved forms of caspase-1 that retain amino acids 371-390.
Catalogue NumberAP1043
Brand Family Calbiochem®
SynonymsAnti-CASP1, Anti-Interleukin-1β convertase, Anti-IL-1BC, Anti-IL-1β-converting enzyme, Anti-ICE, Anti-Interleukin-1β-converting enzyme
Application Data
Detection of human and mouse caspase-1 by immunoblotting. Samples: Whole cell lysate from HeLa (lane A) and NIH3T3 cells (lane B). Primary antibody: Anti-Caspase-1 Mouse mAb (14F468) (Cat. No. AP1043) (0.1 µg/ml, HeLa lysate and 2 µg/ml, NIH3T3 lysate). Detection: chemiluminescence.

Detection of human caspase-1 by staining paraffin sections. Sample: Colon carcinoma, formalin-fixed (panels A and B). Primary antibody: Anti-Caspase-1 Mouse mAb (14F468) (Cat. No. AP1043). Detection: DAB with hematoxylin counterstain.
References
ReferencesTatsuta, T., et al. 2000. J. Biol. Chem. 275, 14248.
Li, P., et al. 1995. Cell 80, 401.
Yuan, J., et al. 1993. Cell 75, 641.
Cerretti, D.P., et al. 1992. Science 256, 97.
Product Information
FormLiquid
FormulationIn PBS.
Positive controlHeLa and NIH 3T3 cells and colon carcinoma
Preservative≤ 0.1% sodium azide
Applications
Key Applications Immunoblotting (Western Blotting)
Paraffin Sections
Application NotesImmunoblotting (0.5-2 µg/ml)
Paraffin Sections (see comments)
Application CommentsWill stain formalin-fixed, paraffin-embedded tissues, but requires titration for individual samples. The immunogen sequence is also conserved in rat caspase-1, but cross-reactivity has not been tested. Also recognizes cleaved forms of caspase-1 that retain amino acids 371-390. Antibody should be titrated for optimal results in individual systems.
Biological Information
Immunogena synthetic peptide (RKVRFSFEQPDGRAQMPTTE) corresponding to amino acids 371-390 of human caspase-1
ImmunogenHuman
Clone14F468
HostMouse
IsotypeIgG₁
Concentration Label Please refer to vial label for lot-specific concentration
Physicochemical Information
Dimensions
Materials Information
Toxicological Information
Safety Information according to GHS
Safety Information
Product Usage Statements
Storage and Shipping Information
Ship Code Blue Ice Only
Toxicity Standard Handling
Storage -20°C
Avoid freeze/thaw Avoid freeze/thaw
Do not freeze Ok to freeze
Special InstructionsFollowing initial thaw, aliquot and freeze (-20°C).
Packaging Information
Transport Information
Supplemental Information
Specifications
Global Trade Item Number
Catalogue Number GTIN
AP1043 0

Documentation

Anti-Caspase-1 Mouse mAb (14F468) Certificates of Analysis

TitleLot Number
AP1043

References

Reference overview
Tatsuta, T., et al. 2000. J. Biol. Chem. 275, 14248.
Li, P., et al. 1995. Cell 80, 401.
Yuan, J., et al. 1993. Cell 75, 641.
Cerretti, D.P., et al. 1992. Science 256, 97.
Data Sheet

Note that this data sheet is not lot-specific and is representative of the current specifications for this product. Please consult the vial label and the certificate of analysis for information on specific lots. Also note that shipping conditions may differ from storage conditions.

Revision20-August-2007 RFH
SynonymsAnti-CASP1, Anti-Interleukin-1β convertase, Anti-IL-1BC, Anti-IL-1β-converting enzyme, Anti-ICE, Anti-Interleukin-1β-converting enzyme
ApplicationImmunoblotting (0.5-2 µg/ml)
Paraffin Sections (see comments)
Application Data
Detection of human and mouse caspase-1 by immunoblotting. Samples: Whole cell lysate from HeLa (lane A) and NIH3T3 cells (lane B). Primary antibody: Anti-Caspase-1 Mouse mAb (14F468) (Cat. No. AP1043) (0.1 µg/ml, HeLa lysate and 2 µg/ml, NIH3T3 lysate). Detection: chemiluminescence.

Detection of human caspase-1 by staining paraffin sections. Sample: Colon carcinoma, formalin-fixed (panels A and B). Primary antibody: Anti-Caspase-1 Mouse mAb (14F468) (Cat. No. AP1043). Detection: DAB with hematoxylin counterstain.
DescriptionProtein G purified mouse monoclonal antibody. Recognizes the ~45 kDa latent form of caspase-1.
BackgroundCaspases are a family of cysteine proteases that play a key role in mediating apoptosis. All caspases are produced as inactive enzymes that require cleavage of a prodomain, followed by a second cleavage to generate the large and small subunits that form the tetrameric, active enzyme. Caspase-1, also known as ICE (IL-1β converting enzyme), was first isolated from monocytic lineage cells, and was so named because it converts the 33 kDa pro-form of IL-1β to the active, 17.5 kDa form via a specific, tightly controlled proteolytic cleavage process.
HostMouse
Immunogen speciesHuman
Immunogena synthetic peptide (RKVRFSFEQPDGRAQMPTTE) corresponding to amino acids 371-390 of human caspase-1
Clone14F468
IsotypeIgG₁
Specieshuman, mouse
Positive controlHeLa and NIH 3T3 cells and colon carcinoma
FormLiquid
FormulationIn PBS.
Concentration Label Please refer to vial label for lot-specific concentration
Preservative≤ 0.1% sodium azide
CommentsWill stain formalin-fixed, paraffin-embedded tissues, but requires titration for individual samples. The immunogen sequence is also conserved in rat caspase-1, but cross-reactivity has not been tested. Also recognizes cleaved forms of caspase-1 that retain amino acids 371-390. Antibody should be titrated for optimal results in individual systems.
Storage Avoid freeze/thaw
-20°C
Do Not Freeze Ok to freeze
Special InstructionsFollowing initial thaw, aliquot and freeze (-20°C).
Toxicity Standard Handling
ReferencesTatsuta, T., et al. 2000. J. Biol. Chem. 275, 14248.
Li, P., et al. 1995. Cell 80, 401.
Yuan, J., et al. 1993. Cell 75, 641.
Cerretti, D.P., et al. 1992. Science 256, 97.