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AP1043 Anti-Caspase-1 Mouse mAb (14F468)

Anti-Caspase-1 Mouse mAb (14F468) MSDS (material safety data sheet) or SDS, CoA and CoQ, dossiers, brochures and other available documents.

Synonyms: Anti-CASP1, Anti-Interleukin-1β convertase, Anti-IL-1BC, Anti-IL-1β-converting enzyme, Anti-ICE, Anti-Interleukin-1β-converting enzyme

AP1043
  
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Overview

Key Spec Table

Host
M
Description
Overview

This product has been discontinued.





Recognizes the ~45 kDa latent form of caspase-1 in HeLa and NIH3T3 cells. Also recognizes cleaved forms of caspase-1 that retain amino acids 371-390.
Catalogue NumberAP1043
Brand Family Calbiochem®
SynonymsAnti-CASP1, Anti-Interleukin-1β convertase, Anti-IL-1BC, Anti-IL-1β-converting enzyme, Anti-ICE, Anti-Interleukin-1β-converting enzyme
Application Data
Detection of human and mouse caspase-1 by immunoblotting. Samples: Whole cell lysate from HeLa (lane A) and NIH3T3 cells (lane B). Primary antibody: Anti-Caspase-1 Mouse mAb (14F468) (Cat. No. AP1043) (0.1 µg/ml, HeLa lysate and 2 µg/ml, NIH3T3 lysate). Detection: chemiluminescence.

Detection of human caspase-1 by staining paraffin sections. Sample: Colon carcinoma, formalin-fixed (panels A and B). Primary antibody: Anti-Caspase-1 Mouse mAb (14F468) (Cat. No. AP1043). Detection: DAB with hematoxylin counterstain.
References
ReferencesTatsuta, T., et al. 2000. J. Biol. Chem. 275, 14248.
Li, P., et al. 1995. Cell 80, 401.
Yuan, J., et al. 1993. Cell 75, 641.
Cerretti, D.P., et al. 1992. Science 256, 97.
Product Information
FormLiquid
FormulationIn PBS.
Positive controlHeLa and NIH 3T3 cells and colon carcinoma
Preservative≤ 0.1% sodium azide
Applications
Key Applications Immunoblotting (Western Blotting)
Paraffin Sections
Application NotesImmunoblotting (0.5-2 µg/ml)
Paraffin Sections (see comments)
Application CommentsWill stain formalin-fixed, paraffin-embedded tissues, but requires titration for individual samples. The immunogen sequence is also conserved in rat caspase-1, but cross-reactivity has not been tested. Also recognizes cleaved forms of caspase-1 that retain amino acids 371-390. Antibody should be titrated for optimal results in individual systems.
Biological Information
Immunogena synthetic peptide (RKVRFSFEQPDGRAQMPTTE) corresponding to amino acids 371-390 of human caspase-1
ImmunogenHuman
Clone14F468
HostMouse
IsotypeIgG₁
Concentration Label Please refer to vial label for lot-specific concentration
Storage and Shipping Information
Ship Code Blue Ice Only
Toxicity Standard Handling
Storage -20°C
Avoid freeze/thaw Avoid freeze/thaw
Do not freeze Ok to freeze
Special InstructionsFollowing initial thaw, aliquot and freeze (-20°C).
Global Trade Item Number
Catalogue Number GTIN
AP1043 0