Millipore Sigma Vibrant Logo
Attention: We have moved. Merck Millipore products are no longer available for purchase on MerckMillipore.com.Learn More

ABT129 Anti-Calponin-1 Antibody

This Anti-Calponin-1 Antibody is validated for use in Western Blotting, IHC(P), Immunofluorescence for the detection of Calponin-1.

Anti-Calponin-1 Antibody MSDS (material safety data sheet) or SDS, CoA and CoQ, dossiers, brochures and other available documents.
Research Category: Cell Structure Research Sub-Category: Cytoskeleton Gene Symbol: CNN1 UniProt Number: P51911
ABT129
100 µg  
Purchase on Sigma-Aldrich

Special Offers

Overview

Replacement Information

Special Offers

100% Performance Guaranteed

Key Spec Table

Species ReactivityKey ApplicationsHostFormatAntibody Type
HWB, IH(P), IFRbAffinity PurifiedPolyclonal Antibody
Description
Catalogue NumberABT129
Replaces04-589
DescriptionAnti-Calponin-1 Antibody
Alternate Names
  • Calponin-1
  • Basic calponin
  • Calponin H1, smooth muscle
Background InformationCalponin 1 is a 34 kDa protein that is predominantly found in smooth muscle tissue. It is a high-affinity actin-binding protein, but also interacts with tropomyosin, and calmodulin to modulate muscle contraction. When bound to F-actin, it inhibits the activity of the actin-activated myosin ATPase. Calponin 1 is regulated by reversible phosphorylation involving PKC and CAMKII kinases, and type 2A phosphatases. In non-muscle cells, calponin 1 may play a role in cell migration, motility, and proliferation.
References
Product Information
FormatAffinity Purified
Control
  • Human aortic smooth muscle tissue
PresentationPurified rabbit polyclonal in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide.
Quality LevelMQ100
Applications
ApplicationThis Anti-Calponin-1 Antibody is validated for use in Western Blotting, IHC(P), Immunofluorescence for the detection of Calponin-1.
Key Applications
  • Western Blotting
  • Immunohistochemistry (Paraffin)
  • Immunofluorescence
Application NotesImmunohistochemistry Analysis: A 1:1,000 dilution from a reprsesentative lot detected Calponin-1 in human seminal vesicle tissue.

Immunofluorescence Analysis: A 1:500 dilution from a representative lot detected Calponin-1 in smooth muscle cells from human seminal vesicle tissue.
Biological Information
ImmunogenKLH-conjugated linear peptide corresponding to the C-terminus of human Calponin-1.
EpitopeC-terminus
ConcentrationPlease refer to the Certificate of Analysis for the lot-specific concentration.
HostRabbit
SpecificityThis antibody recognizes the C-terminus of Calponin-1.
Species Reactivity
  • Human
Species Reactivity NoteDemonstrated to react with Human. Predicted to react with Bovine, Chimpanzee, and Sheep based on 100% sequence homology. Other homologies: Rat (92% sequence homology) and Mouse (83% sequence homology).
Antibody TypePolyclonal Antibody
Entrez Gene Number
Gene Symbol
  • CNN1
Purification MethodAffinity Purfied
UniProt Number
Molecular Weight~33 kDa observed
Physicochemical Information
Dimensions
Materials Information
Toxicological Information
Safety Information according to GHS
Safety Information
Product Usage Statements
Quality AssuranceEvaluated by Western Blot in human aortic smooth muscle tissue.

Western Blot Analysis: 0.1 µg/mL of this antibody detected Calponin-1 in human aortic smooth muscle tissue.
Usage Statement
  • Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
Storage and Shipping Information
Storage ConditionsStable for 1 year at 2-8°C from date of receipt.
Packaging Information
Material Size100 µg
Transport Information
Supplemental Information
Specifications
Global Trade Item Number
Catalogue Number GTIN
ABT129 04053252599927

Documentation

Anti-Calponin-1 Antibody MSDS

Title

Safety Data Sheet (SDS) 

Anti-Calponin-1 Antibody Certificates of Analysis

TitleLot Number
Anti-Calponin-1 2484064
Anti-Calponin-1 - 3227310 3227310
Anti-Calponin-1 - 3439695 3439695
Anti-Calponin-1 - 4108268 4108268
Anti-Calponin-1 - Q2055263 Q2055263
Anti-Calponin-1 -2659365 2659365
Anti-Calponin-1 -2683272 2683272
Anti-Calponin-1 -2799095 2799095
Anti-Calponin-1 Polyclonal Antibody 3054704
Anti-Calponin-1 Polyclonal Antibody 2909628
Show more