Millipore Sigma Vibrant Logo
Attention: We have moved. Merck Millipore products are no longer available for purchase on MerckMillipore.com.Learn More

04-932 Anti-Calmodulin Binding Protein Epitope Tag Antibody, clone C16T, rabbit monoclonal

This Anti-Calmodulin Binding Protein Epitope Tag Antibody, clone C16T is validated for use in WB for the detection of Calmodulin Binding Protein Epitope Tag.

Anti-Calmodulin Binding Protein Epitope Tag Antibody, clone C16T, rabbit monoclonal MSDS (material safety data sheet) or SDS, CoA and CoQ, dossiers, brochures and other available documents.
Research Category: Epitope Tags & General Use Research Sub-Category: Epitope Tags Gene Symbol: CALM, CALML2 , CAM , CAM1, CAM2 , CAM3 , CAMB, CAMC, CAMI, CAMIII, CaM, DD132, PHKD UniProt Number: P62158
04-932
100 µl  
Purchase on Sigma-Aldrich

Special Offers

Overview

Replacement Information

Special Offers

100% Performance Guaranteed

Key Spec Table

Species ReactivityKey ApplicationsHostFormatAntibody Type
VrtWBRbCulture SupernatantMonoclonal Antibody
Description
Catalogue Number04-932
Replaces05-932
DescriptionAnti-Calmodulin Binding Protein Epitope Tag Antibody, clone C16T, rabbit monoclonal
Alternate Names
  • calmodulin 1
  • calmodulin 1 (phosphorylase kinase, delta)
  • phosphorylase kinase, delta subunit
Background InformationCalmodulin Binding Protein (CBP) epitope tags are found in several E. coli expression vectors. Calmodulin (CaM), a small 17 kDa ubiquitous Ca2+ binding protein, acts as an intracellular Ca2+ receptor and transduces Ca2+ transients, usually in response to growth factors. It mediates the activity of a number of Ca2+ regulating enzymes, including protein kinases, phosphatases, nitric oxide synthases, and phosphodiesterase. Calmodulin is the principal intracellular calcium receptor. It is an acidic protein of 148 residues, and upon binding Ca2+, it in turn binds to short peptide sequences of many target proteins. Such interaction is thought to induce a conformational change on the target, resulting in its activation. Targets include the constitutively expressed nitric oxide synthases, the CaM kinases, and PP2B/Calcineurin.
References
Product Information
FormatCulture Supernatant
Control
  • Samples containing the calmodulin binding protein epitope tag.
PresentationCultured supernatant with 0.05% sodium azide.
Quality LevelMQ100
Applications
ApplicationThis Anti-Calmodulin Binding Protein Epitope Tag Antibody, clone C16T is validated for use in WB for the detection of Calmodulin Binding Protein Epitope Tag.
Key Applications
  • Western Blotting
Biological Information
ImmunogenKLH-conjugated, synthetic peptide corresponding to the Calmodulin Binding Protein (CBP) epitope tag found in several E. coli expression vectors.
EpitopeCBP epitope
CloneC16T
HostRabbit
SpecificityRecombinant proteins containing the calmodulin binding protein epitope tag.
IsotypeIgG
Species Reactivity
  • Vertebrates
Antibody TypeMonoclonal Antibody
Entrez Gene SummaryCalmodulin is the archetype of the family of calcium-modulated proteins of which nearly 20 members have been found. They are identified by their occurrence in the cytosol or on membranes facing the cytosol and by a high affinity for calcium. Calmodulin contains 149 amino acids and has 4
calcium-binding domains. Its functions include roles in growth and the cell cycle as well as in signal transduction and the synthesis and release of neurotransmitters.[supplied by OMIM]
Gene Symbol
  • CALM
  • CALML2
  • CAM
  • CAM1
  • CAM2
  • CAM3
  • CAMB
  • CAMC
  • CAMI
  • CAMIII
  • CaM
  • DD132
  • PHKD
UniProt Number
UniProt SummaryFUNCTION: Calmodulin mediates the control of a large number of enzymes and other proteins by Ca2+. Among the enzymes to be stimulated by the calmodulin-Ca2+ complex are a number of protein kinases and phosphatases. Together with CEP110 and centrin, is involved in a genetic pathway that regulates the centrosome cycle and progression through cytokinesis.
SUBUNIT STRUCTURE: Interacts with MYO1C and RRAD By similarity. Interacts with CEP97, CEP110, TTN/titin and SRY.
SUBCELLULAR LOCATION: Cytoplasm › cytoskeleton › spindle. Cytoplasm › cytoskeleton › spindle pole. Note: Distributed throughout the cell during interphase, but during mitosis becomes dramatically localized to the spindle poles and the spindle microtubules.
PTM: Ubiquitination results in a strongly decreased activity By similarity.Phosphorylation results in a decreased activity By similarity.
MISCELLANEOUS: This protein has four functional calcium-binding sites.
SEQUENCE SIMILARITIES: Belongs to the calmodulin family.
Contains 4 EF-hand domains.
Molecular WeightThe molecular weight is related to the tagged protein.
Physicochemical Information
Dimensions
Materials Information
Toxicological Information
Safety Information according to GHS
Safety Information
Product Usage Statements
Quality AssuranceEvaluated by Western Blot on lysates from transformed E. coli.
Western Blot Analysis: 1:2,000 dilution of this antibody detected a recombinant protein containing the calmodulin binding protein epitope tag in lysates from transformed E. coli.
Usage Statement
  • Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
Storage and Shipping Information
Storage ConditionsStable for 1 year at -20ºC from date of receipt.
Handling Recommendations: Upon receipt, and prior to removing the cap, centrifuge the vial and gently mix the solution. Aliquot into microcentrifuge tubes and store at -20°C. Avoid repeated freeze/thaw cycles, which may damage IgG and affect product performance.
Packaging Information
Material Size100 µl
Transport Information
Supplemental Information
Specifications
Global Trade Item Number
Catalogue Number GTIN
04-932 04053252469435

Documentation

Anti-Calmodulin Binding Protein Epitope Tag Antibody, clone C16T, rabbit monoclonal MSDS

Title

Safety Data Sheet (SDS) 

Anti-Calmodulin Binding Protein Epitope Tag Antibody, clone C16T, rabbit monoclonal Certificates of Analysis

TitleLot Number
Anti-Calmodulin Binding Protein Epitope Tag, clone C16T - 2026403 2026403
Anti-Calmodulin Binding Protein Epitope Tag, clone C16T - 2122450 2122450
Anti-Calmodulin Binding Protein Epitope Tag, clone C16T - 2192168 2192168
Anti-Calmodulin Binding Protein Epitope Tag, clone C16T - 2239616 2239616
Anti-Calmodulin Binding Protein Epitope Tag, clone C16T - 3994709 3994709
Anti-Calmodulin Binding Protein Epitope Tag, clone C16T - NRG1674325 NRG1674325
Anti-Calmodulin Binding Protein Epitope Tag, clone C16T Monoclonal Antibody 2971858
Anti-Calmodulin Binding Protein Epitope Tag, clone C16T Monoclonal Antibody 2872332