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MABS513 Anti-CYP7B1 Antibody, clone M17P3F2

Detect CYP7B1 using this mouse monoclonal Anti-CYP7B1 Antibody, clone M17P3F2, Cat. No. MABS513, used in Immunohistochemistry (Paraffin) and Western Blotting.

Anti-CYP7B1 Antibody, clone M17P3F2 MSDS (material safety data sheet) or SDS, CoA and CoQ, dossiers, brochures and other available documents.
Research Category: Signaling Gene Symbol: CYP7B1 UniProt Number: O75881
MABS513
100 μg  
Purchase on Sigma-Aldrich

Overview

Replacement Information

Key Spec Table

Species ReactivityKey ApplicationsHostFormatAntibody Type
HIH(P), WBMPurifiedMonoclonal Antibody
Description
Catalogue NumberMABS513
DescriptionAnti-CYP7B1 Antibody, clone M17P3F2
Alternate Names
  • 25-hydroxycholesterol 7-alpha-hydroxylase
  • EC:1.14.13.100
  • Cytochrome P450 7B1
  • Oxysterol 7-alpha-hydroxylase
Background Information25-hydroxycholesterol 7-alpha-hydroxylase (UniProt: O75881; also known as EC:1.14.13.100, Cytochrome P450 7B1, Oxysterol 7-alpha-hydroxylase) is encoded by the CYP7B1 gene (Gene ID: 9420) in human. CYP7B1 is an enzyme involved in bile acid biosynthesis and shares 40% sequence identity to human cholesterol 7a-hydroxylase (CYP7A1). CYP7B1 localizes to the endoplasmic reticulum membrane and is expressed in brain, testis, ovary, prostate, liver, colon, kidney, and small intestine. It catalyzes the rate-limiting step in the conversion of cholesterol to bile acids. The classic pathway of bile acid synthesis is initiated by cholesterol 7alpha-hydroxylase in the liver and is tightly regulated by bile acid feedback, hormones, and diurnal rhythm. In contrast, the pathway initiated with sterol 27-hydroxylase is located mainly in extrahepatic tissues. An "alternative" pathway of bile acid synthesis begins with 27-hydroxylation of cholesterol by 27-hydroxylase (CYP27), followed by 7alpha-hydroxylation by oxysterol 7alpha-hydroxylase (CYP7B1). Mutations in CYP7B1 have been associated with Spastic paraplegia 5A, an autosomal recessive disorder that is characterized by a slow, gradual, progressive weakness and spasticity of the lower limbs. Disorders in CYP7B1 also cause congenital bile acid synthesis defect 3 (CBAS3) that leads to severe cholestasis, cirrhosis, and liver synthetic failure.
References
Product Information
FormatPurified
PresentationPurified mouse monoclonal antibody IgG1 in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide.
Quality LevelMQ100
Applications
ApplicationDetect CYP7B1 using this mouse monoclonal Anti-CYP7B1 Antibody, clone M17P3F2, Cat. No. MABS513, used in Immunohistochemistry (Paraffin) and Western Blotting.
Key Applications
  • Immunohistochemistry (Paraffin)
  • Western Blotting
Application NotesImmunohistochemistry Analysis: A 1:50-250 dilution from a representative lot detected CYP7B1 in human cerebral cortex and human pancreatic cancer tissue.

Immunohistochemistry Analysis: A representative lot detected CYP7B1 in in human liver tissue. (Courtesy of Ayham Alnabulsi, Ph.D., Vertebrate Antibodies Ltd. United Kingdom).

Western Blotting Analysis: A 1:2,000 dilution from a representative lot detected CYP7B1 in human liver tissue lysate.
Biological Information
ImmunogenOvalbumin-conjugated linear peptide corresponding to 9 amino acids from the C-terminal end of human 25-hydroxycholesterol 7-alpha-hydroxylase (CYP7B1).
CloneM17P3F2
ConcentrationPlease refer to lot specific datasheet.
HostMouse
SpecificityClone M17P3F2 detects CYP7B1 in human cerebral cortex, testis, pancreatic cancer, and colon cancer.
IsotypeIgG1λ
Species Reactivity
  • Human
Species Reactivity NoteHuman.
Antibody TypeMonoclonal Antibody
Entrez Gene Number
Gene Symbol
  • CYP7B1
Purification MethodProtein G purified
UniProt Number
Molecular Weight58.26 kDa calculated.
Physicochemical Information
Dimensions
Materials Information
Toxicological Information
Safety Information according to GHS
Safety Information
Product Usage Statements
Quality AssuranceEvaluated by Immunohistochemistry in human testis tissue.

Immunohistochemistry Analysis: A 1:50 dilution of this antibody detected CYP7B1 in human testis tissue.
Usage Statement
  • Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
Storage and Shipping Information
Storage ConditionsStable for 1 year at 2-8°C from date of receipt.
Packaging Information
Material Size100 μg
Transport Information
Supplemental Information
Specifications
Global Trade Item Number
Catalogue Number GTIN
MABS513 04054839168796