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MABS502 Anti-CYP11B1 Antibody, clone 80-7

Anti-CYP11B1 Antibody, clone 80-7 is an antibody against CYP11B1 for use in Western Blotting and Immunohistochemistry.

Anti-CYP11B1 Antibody, clone 80-7 MSDS (material safety data sheet) or SDS, CoA and CoQ, dossiers, brochures and other available documents.
Research Category: Signaling Research Sub-Category: Signaling Neuroscience Gene Symbol: CYP11B1, S11BH UniProt Number: P15538
MABS502
100 μg  
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Key Spec Table

Species ReactivityKey ApplicationsHostFormatAntibody Type
H, RWB, IHCRPurifiedMonoclonal Antibody
Description
Catalogue NumberMABS502
DescriptionAnti-CYP11B1 Antibody, clone 80-7
Alternate Names
  • Cytochrome P450 11B1, mitochondrial
  • CYPXIB1
  • Cytochrome P-450c11
  • Cytochrome P450C11
  • Steroid 11-beta-hydroxylase
  • CYP11B1
Background InformationCytochrome P450 11B1, mitochondrial (EC1.14.15.4, EC1.14.15.5; UniProt P15538; also known as CYPXIB1, Cytochrome P-450c11, Cytochrome P450C11, Cytochrome P450 subfamily XIB polypeptide 1, Cytochrome P450 XIB1, Steroid 11-beta-monooxygenase, Steroid 11-beta-hydroxylase) is encoded by the CYP11B1 (Also known as CPN1, CYP11B, FHI, P450C11, S11BH) gene in human (Gene ID 1584). CYP11B1 protein is a member of the cytochrome P450 superfamily of monooxygenases that catalyze reactions involved in drug metabolism and synthesis of cholesterol, steroids and other lipids. It is localized to the inner mitochondrial membrane and mediates the conversion of progesterone to cortisol in the adrenal cortex. CYP11B1 gene mutations are known causes of adrenal hyperplasia 4 (AH4), a form of congenital adrenal hyperplasia due to defective synthesis of cortisol.
References
Product Information
FormatPurified
HS Code3002 15 90
PresentationPurified rat monoclonal IgG2aκ antibody in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide.
Quality LevelMQ100
Applications
ApplicationAnti-CYP11B1 Antibody, clone 80-7 is an antibody against CYP11B1 for use in Western Blotting and Immunohistochemistry.
Key Applications
  • Western Blotting
  • Immunohistochemistry
Application NotesWestern Blotting Analysis: 0.5 µg/mL from a representative lot detected exogenously expressed human CYP11B1, but not CYP11B1 in 10 µg of HEK293 cell lysate.
Western Blotting Analysis: A representative lot detected transfected CYP11B1 in HEK293 cells and endogenous CYP11B1 in mitochondrial preparation from human adrenocortical cell line HAC15 (Gomez-Sanchez, C.E., et al. 2014 Mol. Cell. Endocrinol. 383(1-2):111-117).
Immunohistochemistry Analysis: A representative lot detected CYP11B1 in normal human adrenal tissue.
Biological Information
ImmunogenChicken serum albumin-conjugated linear peptide corresponding to human CYP11B1 near the N-terminus.
EpitopeNear N-terminus
Clone80-7
ConcentrationPlease refer to lot specific datasheet.
HostRat
IsotypeIgG2aκ
Species Reactivity
  • Human
  • Rat
Antibody TypeMonoclonal Antibody
Entrez Gene Number
Gene Symbol
  • CYP11B1
  • S11BH
Purification MethodProtein G Purified
UniProt Number
Molecular Weight~52 kDa observed. Uncharacterized band(s) may appear in some lysates.
Physicochemical Information
Dimensions
Materials Information
Toxicological Information
Safety Information according to GHS
Safety Information
Product Usage Statements
Quality AssuranceEvaluated by Western Blotting in PC12 cell lysate.

Western Blotting Analysis: 0.5 µg/mL of this antibody detected CYP11B1 in 10 µg of PC12 cell lysate.
Usage Statement
  • Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
Storage and Shipping Information
Storage ConditionsStable for 1 year at 2-8°C from date of receipt.
Packaging Information
Material Size100 μg
Transport Information
Supplemental Information
Specifications
Global Trade Item Number
Catalogue Number GTIN
MABS502 04055977170702

Documentation

Anti-CYP11B1 Antibody, clone 80-7 MSDS

Title

Safety Data Sheet (SDS) 

Anti-CYP11B1 Antibody, clone 80-7 Certificates of Analysis

TitleLot Number
Anti-CYP11B1, clone 80-7 - 2986028 2986028
Anti-CYP11B1, clone 80-7 - 3249540 3249540
Anti-CYP11B1, clone 80-7 - 3445031 3445031
Anti-CYP11B1, clone 80-7 - 3561545 3561545
Anti-CYP11B1, clone 80-7 - 3610488 3610488
Anti-CYP11B1, clone 80-7 - 3886653 3886653
Anti-CYP11B1, clone 80-7 - 3910239 3910239
Anti-CYP11B1, clone 80-7 - 3979868 3979868
Anti-CYP11B1, clone 80-7 - 4020573 4020573
Anti-CYP11B1, clone 80-7 - 4031368 4031368
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Technical Info

Title
Characterization of Estrogen Receptor α Phosphorylation Sites in Breast Cancer Tissue Using the SNAP i.d® 2.0 System
White Paper: Further considerations of antibody validation and usage.