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DR1105 Anti-CRY1 Mouse mAb (4H4-1C4)

This Anti-CRY1 Mouse mAb (4H4-1C4) is validated for use in Immunoblotting, Immunocytochemistry, Paraffin Sections for the detection of CRY1.

Anti-CRY1 Mouse mAb (4H4-1C4) MSDS (material safety data sheet) or SDS, CoA and CoQ, dossiers, brochures and other available documents.

Synonyms: Anti-Cryptochrome 1

DR1105
  
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Overview

Replacement Information

Key Spec Table

Species ReactivityHostAntibody Type
HMMonoclonal Antibody
Description
OverviewRecognizes the ~60-65 kDa CRY1 protein in HeLa NE cells and human colon adenocarcinoma tissue.
Catalogue NumberDR1105
Brand Family Calbiochem®
SynonymsAnti-Cryptochrome 1
Application Data
Detection of human CRY1 by immunoblotting. Sample: Whole cell lysate from HeLa NE cells. Primary antibody: Anti-CRY1 Mouse mAb (4H4-1C4) (Cat. No. DR1105) (1-5 µg/ml). Detection: chemiluminescence.

Detection of human CRY1 by immunocytochemistry. Sample: HeLa cells fixed in paraformaldehyde. Primary antibody: Anti-CRY1 Mouse mAb (4H4-1C4) (Cat. No. DR1105) (10 µg/ml). Detection: fluorescence.

Detection of human CRY1 by staining paraffin sections. Sample: Formalin-fixed, paraffin-embedded human colon adenocarcinoma tissue. Primary antibody: Anti-CRY1 Mouse mAb (4H4-1C4) (Cat. No. DR1105) (5 µg/ml). Detection: colorimatric (DAB).
References
Product Information
FormLiquid
FormulationIn PBS, pH 7.2.
Positive controlHeLa NE cells, Human colon adenocarcinoma tissue
PreservativeNone
Applications
Key Applications Immunoblotting (Western Blotting)
Immunocytochemistry
Paraffin Sections
Application NotesImmunoblotting (1-5 µg/ml)
Immunocytochemistry (1- µg/ml)
Paraffin Sections (5 µg/ml)
Application CommentsVariables associated with assay conditions will dictate the proper working dilution.
Biological Information
ImmunogenFull-length, recombinant, human CRY1 (aa 1-587), expressed as a GST fusion protein
Clone4H4-1C4
HostMouse
IsotypeIgG₁
Species Reactivity
  • Human
Antibody TypeMonoclonal Antibody
Physicochemical Information
Dimensions
Materials Information
Toxicological Information
Safety Information according to GHS
Safety Information
Product Usage Statements
Storage and Shipping Information
Ship Code Blue Ice Only
Toxicity Regulatory Review
Storage -20°C
Avoid freeze/thaw Avoid freeze/thaw
Do not freeze Ok to freeze
Special InstructionsFollowing initial thaw, aliquot and freeze (-20°C).
Packaging Information
Transport Information
Supplemental Information
Specifications
Global Trade Item Number
Catalogue Number GTIN
DR1105 0

Documentation

Data Sheet

Note that this data sheet is not lot-specific and is representative of the current specifications for this product. Please consult the vial label and the certificate of analysis for information on specific lots. Also note that shipping conditions may differ from storage conditions.

Revision21-July-2010 RFH
SynonymsAnti-Cryptochrome 1
ApplicationImmunoblotting (1-5 µg/ml)
Immunocytochemistry (1- µg/ml)
Paraffin Sections (5 µg/ml)
Application Data
Detection of human CRY1 by immunoblotting. Sample: Whole cell lysate from HeLa NE cells. Primary antibody: Anti-CRY1 Mouse mAb (4H4-1C4) (Cat. No. DR1105) (1-5 µg/ml). Detection: chemiluminescence.

Detection of human CRY1 by immunocytochemistry. Sample: HeLa cells fixed in paraformaldehyde. Primary antibody: Anti-CRY1 Mouse mAb (4H4-1C4) (Cat. No. DR1105) (10 µg/ml). Detection: fluorescence.

Detection of human CRY1 by staining paraffin sections. Sample: Formalin-fixed, paraffin-embedded human colon adenocarcinoma tissue. Primary antibody: Anti-CRY1 Mouse mAb (4H4-1C4) (Cat. No. DR1105) (5 µg/ml). Detection: colorimatric (DAB).
DescriptionPurified mouse monoclonal antibody. Recognizes the ~60-65 kDa CRY1 protein.
HostMouse
ImmunogenFull-length, recombinant, human CRY1 (aa 1-587), expressed as a GST fusion protein
Clone4H4-1C4
IsotypeIgG₁
Specieshuman
Positive controlHeLa NE cells, Human colon adenocarcinoma tissue
FormLiquid
FormulationIn PBS, pH 7.2.
PreservativeNone
CommentsVariables associated with assay conditions will dictate the proper working dilution.
Storage Avoid freeze/thaw
-20°C
Do Not Freeze Ok to freeze
Special InstructionsFollowing initial thaw, aliquot and freeze (-20°C).
Toxicity Regulatory Review