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AB10000 Anti-CHIP/STUB1 Antibody

This Anti-CHIP/STUB1 Antibody is validated for use in ELISA, WB for the detection of CHIP/STUB1.

Anti-CHIP/STUB1 Antibody MSDS (material safety data sheet) or SDS, CoA and CoQ, dossiers, brochures and other available documents.
Research Category: Protein Trafficking Research Sub-Category: Ubiquitin & Ubiquitin Metabolism Gene Symbol: STUB1, SDCCAG7, NY-CO-7, HSPABP2, UBOX1, CHIP UniProt Number: Q9UNE7
AB10000
100 µg  
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Key Spec Table

Species ReactivityKey ApplicationsHostFormatAntibody Type
H, ChpELISA, WBGtAffinity PurifiedPolyclonal Antibody
Description
Catalogue NumberAB10000
Brand Family Chemicon®
Trade Name
  • Chemicon
DescriptionAnti-CHIP/STUB1 Antibody
Alternate Names
  • CHIP
  • STUB1
Background InformationU box domain E3 ubiquitin ligase. CHIP E3 controls both the association of Hsp70/Hsp90 chaperones with ErbB2 and the down-regulation of ErbB2 induced by inhibitors of Hsp90. CHIP-induced degradation was observed for mutant and wild-type p53, which transiently associate with molecular chaperones Hsc70 and Hsp90 and can be diverted onto a degradation pathway through this association. Also, CHIP can interact with the Smad1/Smad4 proteins and block BMP signal transduction through the ubiquitin-mediated degradation of Smad proteins.
References
Product Information
FormatAffinity Purified
Control
  • Positive Control: Highly expressed in brain, heart, skelatal muscle, pancreas and placenta. Weak expression in kidney, liver and lung.
PresentationTris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin.
Quality LevelMQ100
Applications
ApplicationThis Anti-CHIP/STUB1 Antibody is validated for use in ELISA, WB for the detection of CHIP/STUB1.
Key Applications
  • ELISA
  • Western Blotting
Biological Information
ImmunogenPeptide with sequence DAFISENGWVEDY, from the C-terminus of the protein sequence according to NP_005852.
HostGoat
Species Reactivity
  • Human
  • Chimpanzee
Antibody TypePolyclonal Antibody
Entrez Gene Number
Gene Symbol
  • STUB1
  • SDCCAG7
  • NY-CO-7
  • HSPABP2
  • UBOX1
  • CHIP
UniProt Number
UniProt SummaryFUNCTION: SwissProt: Q9UNE7 # Modulates the activity of several chaperone complexes, including Hsp70, Hsc70 and Hsp90. Has E3 ubiquitin-protein ligase activity and targets misfolded chaperone substrates towards proteasomal degradation. Mediates transfer of non-canonical short ubiquitin chains to HSPA8 that have no effect on HSPA8 degradation.
SIZE: 303 amino acids; 34856 Da
SUBUNIT: Interacts with BAG2, and with the E2 ubiquitin conjugating enzymes UBE2D1, UBE2D2 and UBE2D3. Interacts with the C-terminal domains of HSPA8 and HSPA1A. Detected in a ternary complex containing STUB1, HSPA1A and HSPBP1.
SUBCELLULAR LOCATION: Cytoplasm.
TISSUE SPECIFICITY: Highly expressed in skeletal muscle, heart, pancreas, brain and placenta. Detected in kidney, liver and lung.
PTM: Auto-ubiquitinated (in vitro). & Phosphorylated upon DNA damage, probably by ATM or ATR.
DISEASE:SwissProt: Q9UNE7 # Antibodies against STUB1 are found in patients with chronic lymphocytic leukemia (CLL) and in colorectal cancer patients.
SIMILARITY: SwissProt: Q9UNE7 ## Contains 3 TPR repeats. & Contains 1 U-box domain.
Physicochemical Information
Dimensions
Materials Information
Toxicological Information
Safety Information according to GHS
Safety Information
Product Usage Statements
Usage Statement
  • Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
Storage and Shipping Information
Storage ConditionsStore at -20°C.
Packaging Information
Material Size100 µg
Transport Information
Supplemental Information
Specifications
Global Trade Item Number
Catalogue Number GTIN
AB10000 04053252325144

Documentation

Anti-CHIP/STUB1 Antibody MSDS

Title

Safety Data Sheet (SDS) 

Anti-CHIP/STUB1 Antibody Certificates of Analysis

TitleLot Number
GOAT ANTI-STUB1/CHIP POLYCLONAL ANTIBODY - 2344416 2344416
GOAT ANTI-STUB1/CHIP POLYCLONAL ANTIBODY - 4090669 4090669
GOAT ANTI-STUB1/CHIP POLYCLONAL ANTIBODY -2669987 2669987
GOAT ANTI-STUB1/CHIP POLYCLONAL ANTIBODY -2677243 2677243
GOAT ANTI-STUB1/CHIP POLYCLONAL ANTIBODY -2768140 2768140

References

Reference overviewPub Med ID
CHIP is a U?box?dependent E3 ubiquitin ligase: identification of Hsc70 as a target for ubiiquitylation.
Jiang J., et al.
J Biol Chem., 276(46):42938?44 (2001 )  2001

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