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MABS1132 Anti-CDKL5 Antibody, clone 8F3.1

Anti-CDKL5 Antibody, clone 8F3.1 is an antibody against CDKL5 for use in Western Blotting.

Anti-CDKL5 Antibody, clone 8F3.1 MSDS (material safety data sheet) or SDS, CoA and CoQ, dossiers, brochures and other available documents.
Research Category: Signaling Research Sub-Category: Developmental Signaling Gene Symbol: CDKL5, EIEE2, STK9 UniProt Number: O76039
MABS1132
100 μg  
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Key Spec Table

Species ReactivityKey ApplicationsHostFormatAntibody Type
M, RWBMPurifiedMonoclonal Antibody
Description
Catalogue NumberMABS1132
DescriptionAnti-CDKL5 Antibody, clone 8F3.1
Alternate Names
  • Cyclin-dependent kinase-like 5
  • Serine/threonine-protein kinase 9
Background InformationCyclin-dependent kinase-like 5 (EC 2.7.11.22; UniProt O76039; also known as Serine/threonine-protein kinase 9) is encoded by the CDKL5 (also known as EIEE2, STK9) gene (Gene ID 6792) in human. CDKL5/STK9 is a serine/threonine protein kinase widely distributed in all tissues, with highest levels in thymus, testes, and brain, where it is found localized in the nucleus and dendrite of neurons. CDKL5/STK9 expression is found peaked in the early postnatal period and Cdkl5-knockdown in mice leads to autistic-like behavior, dendritic hypotrophy and impairment of neural circuit communication. Likewise, human CDKL5 gene mutations are linked to X-linked neurological developmental disorders involving severe mental retardation and seizures appearing in the first postnatal months. Point mutations in CDKL5 have also been reported in patients with atypical variants of Rett syndrome (RTT), infantile spasms and severe neurodevelopmental retardation.
References
Product Information
FormatPurified
PresentationPurified mouse monoclonal IgG1κ antibody in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide.
Quality LevelMQ100
Applications
ApplicationAnti-CDKL5 Antibody, clone 8F3.1 is an antibody against CDKL5 for use in Western Blotting.
Key Applications
  • Western Blotting
Application NotesWestern Blotting Analysis: 0.5 µg/mL from a representative lot detected CDKL5 in 10 µg of rat hippocampus tissue lysate.
Biological Information
ImmunogenGST-tagged recombinant human CDKL5 internal fragment.
EpitopeInternal.
Clone8F3.1
ConcentrationPlease refer to lot specific datasheet.
HostMouse
SpecificityExpected to react with both spliced isoforms of human CDKL5 reported by UniProt (O76039).
IsotypeIgG1κ
Species Reactivity
  • Mouse
  • Rat
Species Reactivity NoteMouse, Rat. Predicted to react with Human based on 100% sequence homology.
Antibody TypeMonoclonal Antibody
Entrez Gene Number
Gene Symbol
  • CDKL5
  • EIEE2
  • STK9
Purification MethodProtein G Purified
UniProt Number
Molecular Weight~115 kDa observed. 115.5 kDa (isoform 1) and 107.5 kDa (isoform 2) calculated. Uncharacterized band(s) may appear in some lysates.
Physicochemical Information
Dimensions
Materials Information
Toxicological Information
Safety Information according to GHS
Safety Information
Product Usage Statements
Quality AssuranceEvaluated by Western Blotting in mouse cerebellum tissue lysate.

Western Blotting Analysis: 0.5 µg/mL of this antibody detected CDKL5 in 10 µg of mouse cerebellum tissue lysate.
Usage Statement
  • Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
Storage and Shipping Information
Storage ConditionsStable for 1 year at 2-8°C from date of receipt.
Packaging Information
Material Size100 μg
Transport Information
Supplemental Information
Specifications
Global Trade Item Number
Catalogue Number GTIN
MABS1132 04055977333367

Documentation

Anti-CDKL5 Antibody, clone 8F3.1 MSDS

Title

Safety Data Sheet (SDS) 

Anti-CDKL5 Antibody, clone 8F3.1 Certificates of Analysis

TitleLot Number
Anti-CDKL5, clone 8F3.1 - 3261275 3261275
Anti-CDKL5, clone 8F3.1 - 3540375 3540375
Anti-CDKL5, clone 8F3.1 - 3683785 3683785
Anti-CDKL5, clone 8F3.1 - 3764706 3764706
Anti-CDKL5, clone 8F3.1 - 3923047 3923047
Anti-CDKL5, clone 8F3.1 - 4013355 4013355
Anti-CDKL5, clone 8F3.1 -Q2664682 Q2664682
Anti-CDKL5, clone 8F3.1 Monoclonal Antibody 3127636
Anti-CDKL5, clone 8F3.1 Monoclonal Antibody 3043508