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MABT1548-25UG Anti-CD97 Antibody, clone CLB‐CD97/1

Anti-CD97, clone CLB‐CD97/1, Cat. No. MABT1548, is a mouse monoclonal antibody that detects CD97 and is tested for use in ELISA, Flow Cytometry, Immunofluorescence, Immunohistochemistry, Immunoprecipitation, and Inhibition Assay.

MABT1548-25UG
25 μg  
Purchase on Sigma-Aldrich

Overview

Replacement Information
Description
Catalogue NumberMABT1548-25UG
DescriptionAnti-CD97 Antibody, clone CLB‐CD97/1
Alternate Names
  • Adhesion G protein-coupled receptor E5
  • Leukocyte antigen CD97
Background InformationAdhesion G protein-coupled receptor E5 (UniProt: P48960; also known as Leukocyte antigen CD97, CD97) is encoded by the ADGRE5 (also known as CD97) gene (Gene ID: 976) in human. CD97 is a multi-pass, homodimeric, membrane glycoprotein of the G-protein coupled receptor 2 family that is synthesized with a signal peptide (aa 1-20), which is subsequently cleaved off to produce the mature protein that contains four extracellular domains, seven transmembrane domains, and four cytoplasmic domains. The extracellular domain contains five EGF-like domains. The second and third EGF-like domains contain calcium binding sites, which are important in certain ligand interactions that are calcium-dependent. The fourth EGF-like domain mediates binding of chondroitin sulfate. The extracellular domain also contains one Arg-Gly-Asp (RGD) motif that serves as a recognition site for integrin binding. CD97 levels are quickly upregulated upon activation of most leukocytes. It is involved in cell adhesion and migration through interactions with cell surface proteins and components of the extracellular matrix. Three ligands for CD97 have been recognized: CD55 that serves as a negative regulator of complement cascade; chondroitin sulfate, and integrin 5β1. CD97 is broadly expressed and is found on most hematopoietic cells, including activated lymphocytes, monocytes, macrophages, dendritic cells, and granulocytes. Its expression is also observed on smooth muscle cells. Higher expression of CD97 is reported in thyroid, colorectal, esophageal, and pancreatic carcinomas. Its expression levels correlate with dedifferentiation, migration, and invasion in tumor cells. Three isoforms of CD97 have been described that are produced by alternative splicing. (Ref.: Safaee, M., et al. (2013). Int. J. Oncology. 43(5); 1343-1350; Steinert, M., et al. (2002). Am. J. Pathol. 161(5); 1657-1667; Kwakkenbos, MJ., et al. (2002). J. leukocyte Biol. 71(5); 854-862; Hamann, J., et al. (1996). J. Exp. Med. 184(3); 1185-1189).
References
Product Information
FormatPurified
PresentationPurified mouse monoclonal antibody IgG2a in PBS without preservatives.
Quality LevelMQ200
Applications
ApplicationAnti-CD97, clone CLB‐CD97/1, Cat. No. MABT1548, is a mouse monoclonal antibody that detects CD97 and is tested for use in ELISA, Flow Cytometry, Immunofluorescence, Immunohistochemistry, Immunoprecipitation, and Inhibition Assay.
Key Applications
  • Flow Cytometry
  • ELISA
  • Immunohistochemistry
  • Immunofluorescence
  • Immunoprecipitation
  • Inhibition
Application NotesTested Applications

Flow Cytometry Analysis: A representative lot detected CD97 in Flow Cytometry applications (Hamann, J., et al. (1996). 184(3):1185-9; Kwakkenbos, M.J., et al. (2002). J Leukoc Biol. 71(5):854-62).

Inhibition Assay: A representative lot of this antibody inhibited erythrocyte adhesion to CD97-transfected COS cells.(Hamann, J., et al. (1996). 184(3):1185-9; Hamann, J., et al. (1998). Eur J Immunol.;28(5):1701-7).

Immunoprecipitation Analysis: A representative lot immunoprecipitated CD97 in Immunoprecipitation applications (Hamann, J., et al. (1996). 184(3):1185-9).

Immunohistochemistry Applications: A representative lot detected CD97 in Immunohistochemistry applications (Hamann, J., et al. (1999). Arthritis Rheum. 42(4):650-8; Steinert, M., et al. (2002). Am J Pathol. 161(5):1657-67).

Immunofluorescence Analysis: A representative lot detected CD97 in Immunofluorescence applications (Hamann, J., et al. (1998). Eur J Immunol. 28(5):1701-7; Hamann, J., et al. (1999). Arthritis Rheum. 42(4):650-8; Steinert, M., et al. (2002). Am J Pathol. 161(5):1657-67).

ELISA Analysis: A representative lot detected CD97 in ELISA applications (Hamann, J., et al. (1999). Arthritis Rheum. 42(4):650-8; Steinert, M., et al. (2002). Am J Pathol. 161(5):1657-67).

Note: Actual optimal working dilutions must be determined by end user as specimens, and experimental conditions may vary with the end user.
Biological Information
ImmunogenNIH3T3 cells stably expressing human CD97.
EpitopeN-terminal extracellular domain
CloneCLB‐CD97/1
Concentration1.0 mg/mL. Please refer to guidance on suggested starting dilutions and/or titers per application and sample type.
HostMouse
SpecificityClone CLB‐CD97/1 is a mouse monoclonal antibody that detects CD97 (Adhesion G protein-coupled receptor E5).
IsotypeIgG2a
Species Reactivity
  • Human
Species Reactivity NoteHuman.
Antibody TypeMonoclonal Antibody
Entrez Gene Number
Gene Symbol
  • ADGRE5
Purification MethodProtein G purified
UniProt Number
Molecular Weight91.87 kDa calculated.
Physicochemical Information
Dimensions
Materials Information
Toxicological Information
Safety Information according to GHS
Safety Information
Product Usage Statements
Quality AssuranceEvaluated by Flow Cytometry in THP-1 cells.

Flow Cytometry Analysis: 1.0 µg of this antibody detected CD97 in one million THP-1 cells.
Usage Statement
  • Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
Storage and Shipping Information
Storage ConditionsStore at -10°C to -25°C. Handling Recommendations: Upon receipt and prior to removing the cap, centrifuge the vial and gently mix the solution. Aliquot into microcentrifuge tubes and store at -20°C. Avoid repeated freeze/thaw cycles, which may damage IgG and affect product performance.
Packaging Information
Material Size25 μg
Transport Information
Supplemental Information
Specifications
Global Trade Item Number
Catalogue Number GTIN
MABT1548-25UG 04065270647177