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MABD122A4 Anti-CD73, clone AA60-E3-3, Alexa Fluor® 488 conjugate Antibody

MABD122A4
100 µL  
Purchase on Sigma-Aldrich

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Overview

Replacement Information

Key Spec Table

Species ReactivityKey ApplicationsHostFormatAntibody Type
HICCMAlexaFluor®488Monoclonal Antibody
Description
Catalogue NumberMABD122A4
DescriptionAnti-CD73, clone AA60-E3-3, Alexa Fluor® 488 conjugate Antibody
Alternate Names
  • CD-73
  • 5'-nucleotidase
  • 5'-NT
  • ecto-5'-nucleotidase
Background InformationCD73, also known as Ecto-5'-nucleotidase, catalyzes the conversion of purine 5' mononucleotides to nucleosides, the preferred substrate being AMP. The enzyme consists of a dimer of 2 identical 70-kD subunits bound by a glycosyl phosphatidyl inositol linkage to the external face of the plasma membrane. The enzyme is used as a marker of lymphocyte differentiation, being expressed on subsets of B-cells and T-cells. CD73 is also a marker of undifferentiated mesenchymal stem cells and is often used to identified stem cell populations.
References
Product Information
FormatAlexaFluor®488
HS Code3002 15 90
PresentationPurified mouse monoclonal IgG1κ conjugated to Alexa Fluor® 488 in PBS with 0.1% sodium azide and 15mg/ml BSA.
Quality LevelMQ100
Applications
ApplicationThis Anti-CD73, clone AA60-E3-3 is validated for use in Immunocytochemistry for the detection of CD73.
Key Applications
  • Immunocytochemistry
Application NotesEvaluated by Immunocytochemistry on human bone marrow mesenchymal stem cells. Immunocytochemical analysis: A 1:50 dilutions of this antibody detected CD73 on 1X10^6 human adipose and bone marrow mesenchymal stem cells.
Biological Information
ImmunogenWhole Mouse Dermal Lymphatic Endothelial Cells (DLEC)
CloneAA60-E3-3
ConcentrationPlease refer to lot specific datasheet.
HostMouse
IsotypeIgG1κ
Species Reactivity
  • Human
Species Reactivity NoteDemonstrated to react with human. Predicted to work in mouse based on immunogen design.
Antibody TypeMonoclonal Antibody
Entrez Gene Number
Gene Symbol
  • NT5E
  • NT5
  • NTE
Purification MethodProtein G Purified
UniProt Number
Molecular WeightThe unconjugated parent antibody (Catalog No. MABD122) has an observed MW at 63 kDA.
Physicochemical Information
Dimensions
Materials Information
Toxicological Information
Safety Information according to GHS
Safety Information
Product Usage Statements
Quality AssuranceEvaluated by Immunocytochemistry on human adipose mesenchymal stem cells.
Immunocytochemical analysis: A 1:50 dilutions of this antibody detected CD73 on 1X10^6 human adipose and bone marrow mesenchymal stem cells.
Usage Statement
  • Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
Storage and Shipping Information
Storage ConditionsMaintain refrigerated at 2-8ºC protected from light in undiluted aliquots for up to 6 months from date of receipt.
Packaging Information
Material Size100 µL
Transport Information
Supplemental Information
Specifications
Global Trade Item Number
Catalogue Number GTIN
MABD122A4 04053252978814

Documentation

Anti-CD73, clone AA60-E3-3, Alexa Fluor® 488 conjugate Antibody MSDS

Title

Safety Data Sheet (SDS) 

Anti-CD73, clone AA60-E3-3, Alexa Fluor® 488 conjugate Antibody Certificates of Analysis

TitleLot Number
Anti-CD73, clone AA60-E3-3, Alexa Fluor® 488 Conjugate 2860588
Anti-CD73, clone AA60-E3-3, Alexa Fluor® 488 Conjugate 3076896
Anti-CD73, clone AA60-E3-3, Alexa Fluor® 488 Conjugate - Q2436821 Q2436821
Anti-CD73, clone AA60-E3-3, Alexa Fluor® 488 Conjugate - 3327590 3327590
Anti-CD73, clone AA60-E3-3, Alexa Fluor® 488 Conjugate - 3419566 3419566