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MABD122 Anti-CD73 Antibody, clone AA60-E3-3

Detect CD73 using this mouse monoclonal antibody, Anti-CD73 Antibody, clone AA60-E3-3 validated for use in ICC, Flow Cytometry, western blotting & IP.

Anti-CD73 Antibody, clone AA60-E3-3 MSDS (material safety data sheet) or SDS, CoA and CoQ, dossiers, brochures and other available documents.
Research Category: Stem Cell Research Research Sub-Category: Mesenchymal Stem Cells Gene Symbol: NT5E, NT5, NTE UniProt Number: P21589
MABD122
100 µg  
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Key Spec Table

Species ReactivityKey ApplicationsHostFormatAntibody Type
HICC, FC, WB, IPMPurifiedMonoclonal Antibody
Description
Catalogue NumberMABD122
ReplacesMABC668
DescriptionAnti-CD73 Antibody, clone AA60-E3-3
Alternate Names
  • 5'-nucleotidase
  • 5'-NT
  • ecto-5'-nucleotidase
Background InformationCD73, also known as Ecto-5'-nucleotidase, catalyzes the conversion of purine 5' mononucleotides to nucleosides, the preferred substrate being AMP. The enzyme consists of a dimer of 2 identical 70-kD subunits bound by a glycosyl phosphatidyl inositol linkage to the external face of the plasma membrane. The enzyme is used as a marker of lymphocyte differentiation, being expressed on subsets of B-cells and T-cells. CD73 is also a marker of undifferentiated mesenchymal stem cells and is often used to identified stem cell populations.
References
Product Information
FormatPurified
Control
  • Human Adipose Mesenchymal Stem Cells
PresentationPurified mouse monoclonal IgG1κ in buffer containing PBS with 0.05% sodium azide.
Quality LevelMQ100
Applications
ApplicationDetect CD73 using this mouse monoclonal antibody, Anti-CD73 Antibody, clone AA60-E3-3 validated for use in ICC, Flow Cytometry, western blotting & IP.
Key Applications
  • Immunocytochemistry
  • Flow Cytometry
  • Western Blotting
  • Immunoprecipitation
Application NotesThis antibody has been shown to work in immunocytochemical applications on human adipose mesenchymal stem cells. This antibody has been shown to work in western blot applications on HMEC-1 cells by an independant lab.This antibody has been shown to work in immunoprecipitation applications on HMEC-1 cells by an independant lab.
Biological Information
ImmunogenWhole Mouse Dermal Lymphatic Endothelial Cells (DLEC)
CloneAA60-E3-3
ConcentrationPlease refer to the Certificate of Analysis for the lot-specific concentration.
HostMouse
IsotypeIgG1κ
Species Reactivity
  • Human
Species Reactivity NoteDemonstrated to react with human. Predicted to work in mouse based on immunogen design.
Antibody TypeMonoclonal Antibody
Entrez Gene Number
Gene Symbol
  • NT5E
  • NT5
  • NTE
Purification MethodProtein G Purified
UniProt Number
Molecular Weight63 kDa calculated
Physicochemical Information
Dimensions
Materials Information
Toxicological Information
Safety Information according to GHS
Safety Information
Product Usage Statements
Quality AssuranceEvaluated by Flow Cytometry on human adipose mesenchymal stem cells.
Flow Cytometry Analysis: 1 µg/mL of this antibody detected CD73 on 1X10^6 human adipose mesenchymal stem cells.
Usage Statement
  • Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
Storage and Shipping Information
Storage ConditionsStable for 1 year at 2-8°C from date of receipt.
Packaging Information
Material Size100 µg
Transport Information
Supplemental Information
Specifications
Global Trade Item Number
Catalogue Number GTIN
MABD122 04053252862946

Documentation

Anti-CD73 Antibody, clone AA60-E3-3 MSDS

Title

Safety Data Sheet (SDS) 

Anti-CD73 Antibody, clone AA60-E3-3 Certificates of Analysis

TitleLot Number
Anti-CD73, clone AA60-E3-3 - 3228347 3228347
Anti-CD73, clone AA60-E3-3 - 3276090 3276090
Anti-CD73, clone AA60-E3-3 - 3282010 3282010
Anti-CD73, clone AA60-E3-3 - 3318619 3318619
Anti-CD73, clone AA60-E3-3 - 3498496 3498496
Anti-CD73, clone AA60-E3-3 - 4095351 4095351
Anti-CD73, clone AA60-E3-3 - 4103082 4103082
Anti-CD73, clone AA60-E3-3 - Q2153163 Q2153163
Anti-CD73, clone AA60-E3-3 -2569204 2569204
Anti-CD73, clone AA60-E3-3 -2648203 2648203
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