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OP171 Anti-CD63 Mouse mAb (NKI/C-3)

Anti-CD63, mouse monoclonal, clone NKI/C-3, recognizes the heterogeneous 25-110 kDa CD63 in melanomas, clear cell sarcomas, and normal melanocytes. It is validated for WB, & IHC on paraffin sections.

Anti-CD63 Mouse mAb (NKI/C-3) MSDS (material safety data sheet) or SDS, CoA and CoQ, dossiers, brochures and other available documents.
OP171
  
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Overview

Replacement Information

Key Spec Table

Species ReactivityHostAntibody Type
HMMonoclonal Antibody
Description
Overview

This product has been discontinued.



Recognizes the heterogeneous 25-110 kDa CD63 glycoprotein in melanomas, clear cell sarcomas, nevocellular nevi, and normal melanocytes. Also reacts with some mucous-producing tumors, carcinoids, carcinomas of the thyroid, mast cells, histiocytes in tumor regions, and cells with secretory function.
Catalogue NumberOP171
Brand Family Calbiochem®
Application Data
Detection of human CD63 by immunohistochemistry. Sample: Human melanoma fixed in formalin and paraffin-embedded. Primary antibody: Anti-CD63 Mouse mAb (NKI/C-3) (Cat. No. OP171) (1:500). Detection: fluorescence.
References
ReferencesHagen, E.C., et al. 1986. Histopathology 10, 689
Vennegoor, C., et al. 1986. Cancer Immunol. Immunother. 23, 93.
Palmer, A.A., et al. 1985. Pathology 17, 335.
Product Information
FormLiquid
FormulationIn 10 mM PBS, 0.2% BSA, pH 7.4.
Positive controlMelanoma tissue
Preservative≤0.1% sodium azide
Applications
Application ReferencesImmunohistochemistry Stone, CH., et al. 2001. Arch. Pathol. Lab. Med. 125, 751. Bar, H., and Schlote, W. 1997. Clin. Neuropathol. 16, 337. Fernando, S.S., et al. 1994. Pathology 26, 16. Immunoblotting Wang, R., et al. 2001. J. Immunol. Methods 249, 167.
Key Applications Immunoblotting (Western Blotting)
Paraffin Sections
Application NotesImmunoblotting (see application references)
Paraffin Sections (0.2-0.4 µg/ml, heat pre-treatment required; see application references)
Application CommentsExhibits cytoplasmic staining when used in immunohistochemistry. Staining of formalin-fixed, paraffin-embedded tissue sections requires boiling in 10 mM citrate buffer, pH 6.0, for 10-20 min, followed by cooling at room temperature for 20 min. Antibody should be titrated for optimal results in individual systems.
Biological Information
Immunogenplasma membrane fraction of human MeWo cells
ImmunogenHuman
CloneNKI/C-3
HostMouse
IsotypeIgG₁
Species Reactivity
  • Human
Antibody TypeMonoclonal Antibody
Concentration Label Please refer to vial label for lot-specific concentration
Physicochemical Information
Dimensions
Materials Information
Toxicological Information
Safety Information according to GHS
Safety Information
Product Usage Statements
Storage and Shipping Information
Ship Code Blue Ice Only
Toxicity Standard Handling
Storage +2°C to +8°C
Do not freeze Yes
Packaging Information
Transport Information
Supplemental Information
Specifications
Global Trade Item Number
Catalogue Number GTIN
OP171 0

Documentation

Anti-CD63 Mouse mAb (NKI/C-3) MSDS

Title

Safety Data Sheet (SDS) 

Anti-CD63 Mouse mAb (NKI/C-3) Certificates of Analysis

TitleLot Number
OP171

References

Reference overview
Hagen, E.C., et al. 1986. Histopathology 10, 689
Vennegoor, C., et al. 1986. Cancer Immunol. Immunother. 23, 93.
Palmer, A.A., et al. 1985. Pathology 17, 335.
Data Sheet

Note that this data sheet is not lot-specific and is representative of the current specifications for this product. Please consult the vial label and the certificate of analysis for information on specific lots. Also note that shipping conditions may differ from storage conditions.

Revision02-October-2007 RFH
ApplicationImmunoblotting (see application references)
Paraffin Sections (0.2-0.4 µg/ml, heat pre-treatment required; see application references)
Application Data
Detection of human CD63 by immunohistochemistry. Sample: Human melanoma fixed in formalin and paraffin-embedded. Primary antibody: Anti-CD63 Mouse mAb (NKI/C-3) (Cat. No. OP171) (1:500). Detection: fluorescence.
DescriptionProtein G purified mouse monoclonal antibody generated by immunizing BALB/c mice with the specified immunogen and fusing splenocytes with mouse myeloma P3X62Ag8 cells. Recognizes the heterogeneous ~25-110 kDa CD63 glycoprotein.
BackgroundCD63 is a glycosyl phosphatidyl inositol (GPI)-anchored, granulocyte-specific activation antigen whose expression is up-regulated in activated neutrophils. It exists as a heterogeneous 25-110 kDa glycoprotein that is located mainly in the innerside of membranes of cytoplasmic vesicles in melanoma cells. The unglycosylated 25 kDa precursor can be detected in tissue sections by immunohistochemistry. CD63 can be detected in melanomas, clear cell sarcomas (melanoma of soft tissue), nevocellular nevi, and normal melanocytes. It has also been detected in some mucus producing tumors, carcinoids, carcinomas of the thyroid, mast cells, histiocytes in tumor regions, and in secretory cells such as salivary glands, bronchial glands, sweat glands, pancreas, and prostate.
HostMouse
Immunogen speciesHuman
Immunogenplasma membrane fraction of human MeWo cells
CloneNKI/C-3
IsotypeIgG₁
Specieshuman
Positive controlMelanoma tissue
FormLiquid
FormulationIn 10 mM PBS, 0.2% BSA, pH 7.4.
Concentration Label Please refer to vial label for lot-specific concentration
Preservative≤0.1% sodium azide
CommentsExhibits cytoplasmic staining when used in immunohistochemistry. Staining of formalin-fixed, paraffin-embedded tissue sections requires boiling in 10 mM citrate buffer, pH 6.0, for 10-20 min, followed by cooling at room temperature for 20 min. Antibody should be titrated for optimal results in individual systems.
Storage +2°C to +8°C
Do Not Freeze Yes
Toxicity Standard Handling
ReferencesHagen, E.C., et al. 1986. Histopathology 10, 689
Vennegoor, C., et al. 1986. Cancer Immunol. Immunother. 23, 93.
Palmer, A.A., et al. 1985. Pathology 17, 335.
Application referencesImmunohistochemistry Stone, CH., et al. 2001. Arch. Pathol. Lab. Med. 125, 751. Bar, H., and Schlote, W. 1997. Clin. Neuropathol. 16, 337. Fernando, S.S., et al. 1994. Pathology 26, 16. Immunoblotting Wang, R., et al. 2001. J. Immunol. Methods 249, 167.