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ABE1391 Anti-Brd4 Antibody

ABE1391
100 µL  
Purchase on Sigma-Aldrich

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Overview

Replacement Information

Key Spec Table

Species ReactivityKey ApplicationsHostFormatAntibody Type
HWB, ChIP, ICCRbSerumPolyclonal Antibody
Description
Catalogue NumberABE1391
DescriptionAnti-Brd4 Antibody
Alternate Names
  • Bromodomain-containing protein 4
  • Mitotic chromosome-associated protein
  • MCAP
  • Brd4
Background InformationBromodomain-containing protein 4 (Brd4), or Mitotic chromosome-associated protein (MCAP), is a chromatin reader protein and kinase that recognizes and binds acetylated histones and plays a key role in the transmission of epigenetic memory across cell divisions and transcription regulation. Brd4 remains associated with acetylated chromatin throughout the entire cell cycle and provides epigenetic memory for post mitotic G1 gene transcription by preserving acetylated chromatin status and maintaining high-order chromatin structure. During interphase, Brd4 plays a key role in regulating the transcription of signal-inducible genes by associating with the P-TEFb complex and recruiting it to promoters. Brd4 is widely expressed and localized to the nucleus and acetylated chromatin. Brd4 also plays a large role in many cancers, and through its regulation of transcription, Brd4 is important in multiple cellular processes including DNA damage responses, aging, and development.
References
Product Information
FormatSerum
HS Code3002 15 90
PresentationRabbit polyclonal serum containing 0.05% sodium azide.
Quality LevelMQ100
Applications
ApplicationThis Anti-Brd4 Antibody is validated for use in Western Blotting and Chromatin Immunoprecipitation (ChIP) and Immunocytochemistry for the detection of Brd4.
Key Applications
  • Western Blotting
  • Chromatin Immunoprecipitation (ChIP)
  • Immunocytochemistry
Application NotesWestern Blotting Analysis: A representative lot of this antibody was reported to detect Brd4 protein in a HeLa cell lysate (Alsarraj J., et al. (2013). Also see Anup, D., et al. (2003) and Mochizuki, K., et al. (2008) for additional data using mouse embyronic fibroblasts and NIH3T3 cells.

Chromatin Immunoprecipitation (ChIP): A representative lot of this antibody was reported to work in ChIP using chromatin from NIH3T3 cells. See Mochizuki, K., et al. (2008) and Anup, D., et al. (2009).

Immunocytochemistry: A representative lot of this antibody was reported to work in ICC. See Anup, D., et al. (2000) and Anup, D., et al. (2009).
Biological Information
ImmunogenRecombinant protein corresponding to Mouse Brd4 short form.
EpitopeRegion between amino acids 150 to 290 of human Brd4
ConcentrationPlease refer to lot specific datasheet.
HostRabbit
SpecificityRecognizes human Brd4
Species Reactivity
  • Human
Species Reactivity NoteHuman. Predicted to react with mouse based on sequence homology.
Antibody TypePolyclonal Antibody
Entrez Gene Number
Gene Symbol
  • Brd4
  • Mcap
Purification MethodUnpurified
UniProt Number
Molecular Weight~ 100 kDa observed
This antibody primarily detects 100 kd short form of Brd4 and to lesser extent larger 180 kD form (see Alsarraj J., et al. (2013 for additional details on performance of this antibody).
Physicochemical Information
Dimensions
Materials Information
Toxicological Information
Safety Information according to GHS
Safety Information
Product Usage Statements
Quality AssuranceEvaluated by Western Blotting in HEK293 cells.

Western Blotting Analysis: 1:1,000 dilution of this antibody detected Brd4 in 10 µg of HEK293 cell lysate.
Usage Statement
  • Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
Storage and Shipping Information
Storage ConditionsStable for 1 year at -20°C from date of receipt.
Handling Recommendations: Upon receipt and prior to removing the cap, centrifuge the vial and gently mix the solution. Aliquot into microcentrifuge tubes and store at -20°C. Avoid repeated freeze/thaw cycles, which may damage IgG and affect product performance.
Packaging Information
Material Size100 µL
Transport Information
Supplemental Information
Specifications
Global Trade Item Number
Catalogue Number GTIN
ABE1391 04053252993046

Documentation

Anti-Brd4 Antibody MSDS

Title

Safety Data Sheet (SDS) 

Anti-Brd4 Antibody Certificates of Analysis

TitleLot Number
Anti-Brd4 - Q2452913 Q2452913
Anti-Brd4 - 3013367 3013367
Anti-Brd4 - 3782289 3782289
Anti-Brd4 -2677909 2677909
Anti-Brd4 -2742258 2742258
Anti-Brd4 -2805346 2805346
Anti-Brd4 Polyclonal Antibody 3123646
Anti-Brd4 Polyclonal Antibody 2887870
Anti-Brd4 Polyclonal Antibody 2931009

References

Reference overviewPub Med ID
BRD4 short isoform interacts with RRP1B, SIPA1 and components of the LINC complex at the inner face of the nuclear membrane.
Alsarraj, Jude, et al.
PLoS ONE, 8: e80746 (2013)  2013

Show Abstract
24260471 24260471
The bromodomain protein Brd4 insulates chromatin from DNA damage signalling.
Floyd, SR; Pacold, ME; Huang, Q; Clarke, SM; Lam, FC; Cannell, IG; Bryson, BD; Rameseder, J; Lee, MJ; Blake, EJ; Fydrych, A; Ho, R; Greenberger, BA; Chen, GC; Maffa, A; Del Rosario, AM; Root, DE; Carpenter, AE; Hahn, WC; Sabatini, DM; Chen, CC; White, FM; Bradner, JE; Yaffe, MB
Nature  498  246-50  2013

Show Abstract
23728299 23728299
Brd4 marks select genes on mitotic chromatin and directs postmitotic transcription.
Dey, Anup, et al.
Mol. Biol. Cell, 20: 4899-909 (2009)  2009

Show Abstract
19812244 19812244
The bromodomain protein Brd4 stimulates G1 gene transcription and promotes progression to S phase.
Mochizuki, Kazuki, et al.
J. Biol. Chem., 283: 9040-8 (2008)  2008

Show Abstract
18223296 18223296
BRD4 bromodomain gene rearrangement in aggressive carcinoma with translocation t(15;19).
French, CA; Miyoshi, I; Aster, JC; Kubonishi, I; Kroll, TG; Dal Cin, P; Vargas, SO; Perez-Atayde, AR; Fletcher, JA
The American journal of pathology  159  1987-92  2001

Show Abstract
11733348 11733348
A bromodomain protein, MCAP, associates with mitotic chromosomes and affects G(2)-to-M transition.
Dey, A, et al.
Mol. Cell. Biol., 20: 6537-49 (2000)  1999

Show Abstract
10938129 10938129

Technical Info

Title
White Paper - The Message in the Marks: Deciphering Cancer Epigenetics