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ABE1961 Anti-Borealin

Anti-Borealin Antibody, Cat. No. ABE1961, is a highly specific rabbit polyclonal antibody that targets Borealin and has been tested in Immunofluorescence, Immunoprecipitation, and Western Blotting.

Anti-Borealin MSDS (material safety data sheet) or SDS, CoA and CoQ, dossiers, brochures and other available documents.
Research Category: Epigenetics & Nuclear Function Gene Symbol: CDCA8, PESCRG3 UniProt Number: Q53HL2
ABE1961
50 µL  
Purchase on Sigma-Aldrich

Overview

Replacement Information

Key Spec Table

Species ReactivityKey ApplicationsHostFormatAntibody Type
HWB, IF, IPRbSerumPolyclonal Antibody
Description
Catalogue NumberABE1961
DescriptionAnti-Borealin
Alternate Names
  • Cell division cycle-associated protein 8
  • Dasra-B
  • hDasra-B
  • Pluripotent embryonic stem cell-related gene 3 protein
Background InformationBorealin (UniProt: Q53HL2; also known as Cell division cycle-associated protein 8; Dasra-B; hDasra-B; Pluripotent embryonic stem cell-related gene 3 protein) is encoded by the CDCA8 (also known as PESCRG3) gene (Gene ID: 55143) in human. Borealin is a novel 31-kDa chromosomal passenger protein that is conserved among all vertebrates. It is a member of the chromosomal passenger holocomplex along with Aurora B, INCENP, and survivin. This complex play a vital role at the centromere to ensure proper chromosome alignment and segregation. Borealin is required for stability of the bipolar mitotic spindle and for kinetochore error correction. Borealin levels are reported to increase during G2/M phase and are reduced following exit from the M phase of cell cycle. Depletion of Borealin by RNAi is shown to retard mitotic progression and cause spindle T misattachment and abnormalities. Borealin-depleted cells are shown to align their chromosomes to a near normal metaphase plate, but they abruptly undergo multipolar anaphases.
References
Product Information
FormatSerum
PresentationRabbit polyclonal antibody serum with 0.05% sodium azide.
Quality LevelMQ100
Applications
ApplicationAnti-Borealin Antibody, Cat. No. ABE1961, is a highly specific rabbit polyclonal antibody that targets Borealin and has been tested in Immunofluorescence, Immunoprecipitation, and Western Blotting.
Key Applications
  • Western Blotting
  • Immunofluorescence
  • Immunoprecipitation
Application NotesImmunofluorescence Analysis: A representative lot detected Borealin in HeLa cells (Gassmann, R., et. al. (2004). J Cell Biol. 166(2):179-91).

Western Blotting Analysis: A representative lot detected Borealin in HeLa cells (Gassmann, R., et. al. (2004). J Cell Biol. 166(2):179-91).

Immunoprecipitation Analysis: A representative lot detected Borealin in HeLa cells (Gassmann, R., et. al. (2004). J Cell Biol. 166(2):179-91).
Biological Information
ImmunogenA His-tagged full length human recombinant Borealin.
Epitopeunknown
ConcentrationPlease refer to lot specific datasheet.
HostRabbit
SpecificityThis polyclonal antibody detects Borealin in nocodozole treated HeLa cells.
Species Reactivity
  • Human
Species Reactivity NoteHuman.
Antibody TypePolyclonal Antibody
Entrez Gene Number
Gene Symbol
  • CDCA8
  • PESCRG3
Purification MethodUnpurified
UniProt Number
Molecular Weight~36 kDa observed; 31.32 kDa calculated. Uncharacterized bands may be observed in some lysate(s).
Physicochemical Information
Dimensions
Materials Information
Toxicological Information
Safety Information according to GHS
Safety Information
Product Usage Statements
Quality AssuranceEvaluated by Western Blotting in Hela cell lysate treated with Nocodozole.

Western Blotting Analysis: A 1:5,000 dilution of this antibody detected Borealin in 10 µg of Hela cell lysate treated with Nocodozole.
Usage Statement
  • Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
Storage and Shipping Information
Storage ConditionsStable for 1 year at -20°C from date of receipt. Handling Recommendations: Upon receipt and prior to removing the cap, centrifuge the vial and gently mix the solution. Aliquot into microcentrifuge tubes and store at -20°C. Avoid repeated freeze/thaw cycles, which may damage IgG and affect product performance.
Packaging Information
Material Size50 µL
Transport Information
Supplemental Information
Specifications
Global Trade Item Number
Catalogue Number GTIN
ABE1961 04054839154355