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05-449-C Anti-Bin1, clone 99D (Ascites Free) Antibody

This Anti-Bin1, clone 99D (Ascites Free) Antibody is validated for use in Western Blotting and Immunohistochemistry and Immunoprecipitation and Flow Cytometry and Immunofluorescence for the detection of Bin1.

Anti-Bin1, clone 99D (Ascites Free) Antibody MSDS (material safety data sheet) or SDS, CoA and CoQ, dossiers, brochures and other available documents.
Research Category: Apoptosis & Cancer Research Sub-Category: Apoptosis - Additional Gene Symbol: BIN1, AMPHL UniProt Number: O00499
05-449-C
100 µg  
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Key Spec Table

Species ReactivityKey ApplicationsHostFormatAntibody Type
H, Ch, MWB, IHC, IP, FC, IFMPurifiedMonoclonal Antibody
Description
Catalogue Number05-449-C
DescriptionAnti-Bin1, clone 99D (Ascites Free) Antibody
Alternate Names
  • Myc box-dependent-interacting protein 1
  • Amphiphysin II
  • Amphiphysin-like protein
  • Box-dependent myc-interacting protein 1
  • Bridging integrator 1
  • Bin1
Background InformationBin1, also known as Myc box-dependent-interacting protein 1, Amphiphysin II, Amphiphysin-like protein, Box-dependent myc-interacting protein 1, Bridging integrator 1, and encoded by the gene BIN1/AMPHL, is another protein involved in the regulation of synaptic vesicle endocytosis and interestingly also acts as tumor suppressor and inhibits malignant cell transformation. BIN1 is expressed in many tissues but has the highest expression in brain and muscle. BIN1 has isoforms and particular isoforms are expressed in particular tissues. For instance BIN1 isoform 11A is only expressed in the brain and found in axon initial segments and nodes of Ranvier. Mutations in BIN1 are associated with a disordered called centronuclear 2 myopathy. BIN1 is localized to nucleus but some isoforms are associated with cytoplasm.
References
Product Information
FormatPurified
PresentationPurified mouse monoclonal IgG2bκ in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide.
Quality LevelMQ100
Applications
ApplicationThis Anti-Bin1, clone 99D (Ascites Free) Antibody is validated for use in Western Blotting and Immunohistochemistry and Immunoprecipitation and Flow Cytometry and Immunofluorescence for the detection of Bin1.
Key Applications
  • Western Blotting
  • Immunohistochemistry
  • Immunoprecipitation
  • Flow Cytometry
  • Immunofluorescence
Application NotesImmunohistochemistry Analysis: A 1:50 dilution from a representative lot detected Bin1 in human cerebellum and human skeletal muscle myocytes tissue.
Western Blotting Analysis: A representative lot detected Bin1 in primary chicken embryo fibroblasts (Telfer, J.F., et al. (2005). Cellular Signalling. 17:701-8).
Immunoprecipitation Analysis: A representative lot immunoprecipitated Bin1 in C2C12 cell lysate (Wechsler-Reya, R., et al. (1997). Cancer Res. 57:3258-63).
Western Blotting Analysis: A representative lot detected Bin1 in human and rodent cell lines (Wechsler-Reya, R., et al. (1997). Cancer Res. 57:3258-63).
Immunoprecipitation Analysis: A representative lot immunoprecipitated Bin1 in co-immunoprecipitation studies (Sakamuro, D., et al. (1996). Nat. Genet. 14:69-77).
Immunohistochemistry Analysis: A representative lot detected Bin1 in breast and liver carcinoma tissues (Sakamuro, D., et al. (1996). Nat. Genet. 14:69-77).
Immunoprecipitation Analysis: A representative lot immunoprecipitated Bin1 in C2C12 cell lysate (Wechsler-Reya, R. J., et al. (1998). Mol Cell Biol. 18:566-75).
Western Blotting Analysis: A representative lot detected Bin1 in C2C12 cell lysate (Wechsler-Reya, R. J., et al. (1998). Mol Cell Biol. 18:566-75).
Flow Cytometry Analysis: A representative lot detected Bin1 in C2C12 cell lysate (Wechsler-Reya, R. J., et al. (1998). Mol Cell Biol. 18:566-75).
Immunofluorescence Analysis: A representative lot detected Bin1 in C2C12 cells (Wechsler-Reya, R. J., et al. (1998). Mol Cell Biol. 18:566-75).
Biological Information
ImmunogenLinear peptide corresponding to human Bin1.
Clone99D
Concentration1 mg/mL
HostMouse
IsotypeIgG2bκ
Species Reactivity
  • Human
  • Chicken
  • Mouse
Antibody TypeMonoclonal Antibody
Entrez Gene Number
Gene Symbol
  • BIN1
  • AMPHL
Purification MethodProtein G Purified
UniProt Number
Molecular Weight~60 kDa observed
Physicochemical Information
Dimensions
Materials Information
Toxicological Information
Safety Information according to GHS
Safety Information
Product Usage Statements
Quality AssuranceEvaluated by Western Blotting in human skeletal muscle tissue lysate.

Western Blotting Analysis: 1.0 µg/mL of this antibody detected Bin1 in 10 µg of human skeletal muscle tissue lysate.
Usage Statement
  • Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
Storage and Shipping Information
Storage ConditionsStable for 1 year at 2-8°C from date of receipt.
Packaging Information
Material Size100 µg
Transport Information
Supplemental Information
Specifications
Global Trade Item Number
Catalogue Number GTIN
05-449-C 04053252978746

Documentation

Anti-Bin1, clone 99D (Ascites Free) Antibody MSDS

Title

Safety Data Sheet (SDS) 

Anti-Bin1, clone 99D (Ascites Free) Antibody Certificates of Analysis

TitleLot Number
Anti-Bin1, clone 99D (Ascites Free) - Q2435642 Q2435642
Anti-Bin1, clone 99D (Ascites Free) - 3299962 3299962
Anti-Bin1, clone 99D (Ascites Free) - 3538142 3538142
Anti-Bin1, clone 99D (Ascites Free) - 3956980 3956980
Anti-Bin1, clone 99D (Ascites Free) -2707213 2707213
Anti-Bin1, clone 99D (Ascites Free) Monoclonal Antibody 3137648
Anti-Bin1, clone 99D (Ascites Free) Monoclonal Antibody 2979478