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AM53 Anti-Bim Rat mAb (14A8)

Anti-Bim Rat mAb (14A8) MSDS (material safety data sheet) or SDS, CoA and CoQ, dossiers, brochures and other available documents.

Synonyms: Anti-Bcl-2 Interacting Mediator

AM53
  
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Overview

Replacement Information

Key Spec Table

Host
R
Description
Overview

This product has been discontinued.





Recognizes the ~18 kDa Bim, ~20 kDa BimL, and the ~24 kDa BimEL isoforms of Bim in MCF-7 cells.
Catalogue NumberAM53
Brand Family Calbiochem®
SynonymsAnti-Bcl-2 Interacting Mediator
References
ReferencesPuthalakath, H., et al. 1999. Mol. Cell 3, 287.
O’Connor, L., et al. 1998. EMBO J. 17, 384.
Product Information
FormLiquid
FormulationIn 0.05 M sodium phosphate buffer, 0.2% gelatin.
Negative controlNormal rat IgG
Positive controlMCF7 cells or Bim overexpressing cell lines
Preservative≤0.1% sodium azide and 0.05% Bronidox
Applications
Application ReferencesImmunblotting O'Reilly et al. 2000. Am. J. Pathol. 157, 449. Kajihara, T. et al. 2006. Mol. Endocrinol. 20, 2444.
Key Applications Immunoblotting (Western Blotting)
Immunofluorescence
Immunoprecipitation
Application NotesImmunoblotting (1-2 µg/ml; see application references)
Immunofluorescence (0.5 µg/ml, see comments)
Immunoprecipitation (1-2 µg/reaction)
Application CommentsAnti-Bim recognizes an epitope that is common to all 3 Bim isoforms. Permeabilization of the cell membrane is necessary for Bim detection by immunofluorescence. Bim is a cytoplasmic protein present at low levels; to immunoprecipitate Bim, use 1-5 mg of total cell protein. Antibody should be titrated for optimal results in individual systems.
Biological Information
Immunogenrecombinant, mouse Bim
ImmunogenMouse
Clone14A8
HostRat
IsotypeIgG2a
Concentration Label Please refer to vial label for lot-specific concentration
Physicochemical Information
Dimensions
Materials Information
Toxicological Information
Safety Information according to GHS
Safety Information
Product Usage Statements
Storage and Shipping Information
Ship Code Blue Ice Only
Toxicity Standard Handling
Storage +2°C to +8°C
Do not freeze Yes
Packaging Information
Transport Information
Supplemental Information
Specifications
Global Trade Item Number
Catalogue Number GTIN
AM53 0

Documentation

Anti-Bim Rat mAb (14A8) MSDS

Title

Safety Data Sheet (SDS) 

Anti-Bim Rat mAb (14A8) Certificates of Analysis

TitleLot Number
AM53

References

Reference overview
Puthalakath, H., et al. 1999. Mol. Cell 3, 287.
O’Connor, L., et al. 1998. EMBO J. 17, 384.

Brochure

Title
Caspases and other Apoptosis Related Tools Brochure

Citations

Title
Kajihara, T. et al. 2006. Mol. Endocrinol. 20, 2444.
Data Sheet

Note that this data sheet is not lot-specific and is representative of the current specifications for this product. Please consult the vial label and the certificate of analysis for information on specific lots. Also note that shipping conditions may differ from storage conditions.

Revision15-September-2009 RFH
SynonymsAnti-Bcl-2 Interacting Mediator
ApplicationImmunoblotting (1-2 µg/ml; see application references)
Immunofluorescence (0.5 µg/ml, see comments)
Immunoprecipitation (1-2 µg/reaction)
DescriptionPurified rat monoclonal antibody. Recognizes the ~18 kDa Bim, ~20 kDa BimL, and the ~24 kDa BimEL isoforms.
BackgroundBim, Bcl-2 interacting mediator of cell death, mRNA is differentially spliced to code for 3 isoforms of the Bim protein: Bim-110 amino acids, BimL-140 amino acids and BimEL-196 amino acids. The Bim proteins share a 9 amino acid BH3 motif with bcl-2 homologues and this BH3 region is required for bcl-2 binding. Bim proteins also bind to bcl-xL and bcl-w, but not to Adenovirus E1B19K and Epstein-Barr virus BHRF-1. In healthy cells, BimL binds to the cytoplasmic dynein light chain LC8 and is thereby associated with the microtubule associated dynein motor complex. An apoptotic stimulus that disrupts LC8 and the dynein motor complex allows Bim to interact with and neutralize bcl-2.
HostRat
Immunogen speciesMouse
Immunogenrecombinant, mouse Bim
Clone14A8
IsotypeIgG2a
Specieshuman, mouse
Positive controlMCF7 cells or Bim overexpressing cell lines
Negative controlNormal rat IgG
FormLiquid
FormulationIn 0.05 M sodium phosphate buffer, 0.2% gelatin.
Concentration Label Please refer to vial label for lot-specific concentration
Preservative≤0.1% sodium azide and 0.05% Bronidox
CommentsAnti-Bim recognizes an epitope that is common to all 3 Bim isoforms. Permeabilization of the cell membrane is necessary for Bim detection by immunofluorescence. Bim is a cytoplasmic protein present at low levels; to immunoprecipitate Bim, use 1-5 mg of total cell protein. Antibody should be titrated for optimal results in individual systems.
Storage +2°C to +8°C
Do Not Freeze Yes
Toxicity Standard Handling
ReferencesPuthalakath, H., et al. 1999. Mol. Cell 3, 287.
O’Connor, L., et al. 1998. EMBO J. 17, 384.
CitationKajihara, T. et al. 2006. Mol. Endocrinol. 20, 2444.
Application referencesImmunblotting O'Reilly et al. 2000. Am. J. Pathol. 157, 449. Kajihara, T. et al. 2006. Mol. Endocrinol. 20, 2444.