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MABS1890 Anti-BMPR-II Antibody, clone 9F4.1

Anti-BMPR-II, clone 9F4.1, Cat. No. MABS1890, is a mouse monoclonal antibody that detects Bone morphogenetic protein receptor type-2 and has been tested for use in Western Blotting and Immunohistochemistry (Paraffin).

Anti-BMPR-II Antibody, clone 9F4.1 MSDS (material safety data sheet) or SDS, CoA and CoQ, dossiers, brochures and other available documents.
Research Category: Signaling Gene Symbol: BMPR2, PPH1 UniProt Number: Q13873
MABS1890
100 μg  
Purchase on Sigma-Aldrich

Overview

Replacement Information

Key Spec Table

Species ReactivityKey ApplicationsHostFormatAntibody Type
HWB, IH(P)MPurifiedMonoclonal Antibody
Description
Catalogue NumberMABS1890
DescriptionAnti-BMPR-II Antibody, clone 9F4.1
Alternate Names
  • Bone morphogenetic protein receptor type-2
  • EC: 2.7.11.30
  • BMP type-2 receptor
  • BMPR-2
  • Bone morphogenetic protein receptor type II
  • BMP type II receptor
Background InformationBone morphogenetic protein receptor type-2 (UniProt: Q13873; also known as EX: 2.7.11.30, BMP type-2 receptor, BMPR-2, Bone morphogenetic protein receptor type II, BMP type II receptor, BMPR-II) is encoded by the BMPR2 (also known as PPH1) gene (Gene ID: 659) in human. BMPR-2 is a single-pass type I membrane protein that is highly expressed in heart and liver. It binds Bone morphogenetic proteins that are involved in paracrine signaling. Two isoforms of BMPR-2 have been described that are produced by alternative splicing. BMPR-2 serves as a serine/threonine receptor kinase and upon ligand binding it forms a receptor complex consisting of two type II and two type I transmembrane serine/threonine kinases. Type II receptors phosphorylate and activate type I receptors, which autophosphorylate, then bind and activate SMAD transcriptional regulators. It is shown to bind to BMP7, BMP2 and, less efficiently, BMP4. BMPR-2 is synthesized with a signal peptide (aa 1-16), which is subsequently cleaved off. The mature form has an extracellular domain (aa 27-150), a transmembrane domain (aa 151-171), and a cytoplasmic domain (aa 172-1038). Its kinase activity is localized within amino acids 203-504 in the cytoplasmic domain. Mutations in BMPR2 gene are reported to cause primary pulmonary hypertension that is characterized by plexiform lesions of proliferating endothelial cells in pulmonary arterioles. The lesions lead to elevated pulmonary arterial pression, right ventricular failure, and death.
References
Product Information
FormatPurified
PresentationPurified mouse monoclonal antibody IgG1 in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide.
Quality LevelMQ100
Applications
ApplicationAnti-BMPR-II, clone 9F4.1, Cat. No. MABS1890, is a mouse monoclonal antibody that detects Bone morphogenetic protein receptor type-2 and has been tested for use in Western Blotting and Immunohistochemistry (Paraffin).
Key Applications
  • Western Blotting
  • Immunohistochemistry (Paraffin)
Application NotesImmunohistochemistry Analysis: A 1:50-250 dilution from a representative lot detected BMPR-II in human lymphoma, human pancreas, and human cervical cancer tissues.
Biological Information
ImmunogenGST/His-tagged recombinant fragment corresponding to 111 amino acids from the C-terminal half of human BMP type-2 receptor.
Clone9F4.1
ConcentrationPlease refer to lot specific datasheet.
HostMouse
SpecificityClone 9F4.1 detects BMP type-2 receptor in human cells. It targets an epitope within 111 amino acids from the C-terminal half.
IsotypeIgG1κ
Species Reactivity
  • Human
Species Reactivity NoteHuman.
Antibody TypeMonoclonal Antibody
Entrez Gene Number
Gene Symbol
  • BMPR2
  • PPH1
Purification MethodProtein G purified
UniProt Number
Molecular Weight~115 kDa observed, 115.20 kDa calculated. Uncharacterized bands may be observed in some lysate(s).
Physicochemical Information
Dimensions
Materials Information
Toxicological Information
Safety Information according to GHS
Safety Information
Product Usage Statements
Quality AssuranceEvaluated by Western Blotting in HepG2 cell lysate.

Western Blotting Analysis: 0.5 µg/mL of this antibody detected BMPR-II in 10 µg of HepG2 cell lysate.
Usage Statement
  • Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
Storage and Shipping Information
Storage ConditionsStable for 1 year at 2-8°C from date of receipt.
Packaging Information
Material Size100 μg
Transport Information
Supplemental Information
Specifications
Global Trade Item Number
Catalogue Number GTIN
MABS1890 04054839410512