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MABS501 Anti-Anoctamin-5 Antibody, clone 5F7

This Anti-Anoctamin-5 Antibody, clone 5F7 is validated for use in Western Blotting for the detection of Anoctamin-5.

Anti-Anoctamin-5 Antibody, clone 5F7 MSDS (material safety data sheet) or SDS, CoA and CoQ, dossiers, brochures and other available documents.
Research Category: Signaling Research Sub-Category: Signaling Neuroscience Gene Symbol: ANO5, GDD1, TMEM16E UniProt Number: Q75V66
MABS501
200 µL  
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Key Spec Table

Species ReactivityKey ApplicationsHostFormatAntibody Type
M, HWBMUnpurifiedMonoclonal Antibody
Description
Catalogue NumberMABS501
DescriptionAnti-Anoctamin-5 Antibody, clone 5F7
Alternate Names
  • Anoctamin-5
  • Gnathodiaphyseal dysplasia 1 protein
  • Transmembrane protein 16E
Background InformationAnoctamin-5 (UniProt Q75V66; also known as Gnathodiaphyseal dysplasia 1 protein, Integral membrane protein GDD1, Transmembrane protein 16E) is encoded by the ANO5 (also known as GDD1, LGMD2L, TMEM16E) gene (Gene ID 203859) in human. The human Anoctamins (ANO) compose a family of at least ten proteins, all exhibiting eight transmembrane domains and a DUF590 domain of unknown function. The ANO1, ANO2, ANO6, ANO8, and ANO9 are characterized to code for the elusive calcium-activated chloride channels (CaCCs). While the physiological function of anoctamin-5 is not yet characterized, dominant mutations in ANO5 have been found in the rare skeletal disorder gnathodiaphyseal dysplasia. In addition, recessive mutations in ANO5 are found to cause proximal muscular dystrophy (LGMD2L) and distal Miyoshi myopathy (MMD3).
References
Product Information
FormatUnpurified
PresentationMouse monoclonal IgMκ in buffer containing supernatant without preservatives.
Quality LevelMQ100
Applications
ApplicationThis Anti-Anoctamin-5 Antibody, clone 5F7 is validated for use in Western Blotting for the detection of Anoctamin-5.
Key Applications
  • Western Blotting
Application NotesWestern Blotting Analysis: A 1:100 dilution from a representative lot detected Anoctamin-5 in 10 µg of mouse leg muscle tissue lysate.
Biological Information
ImmunogenLinear peptide corresponding to mouse Anoctamin-5 near the C-terminus.
EpitopeNear C-terminus
Clone5F7
ConcentrationPlease refer to lot specific datasheet.
HostMouse
IsotypeIgMκ
Species Reactivity
  • Mouse
  • Human
Species Reactivity NoteMouse, Human. Predicted to react with Rat based on 100% sequence homology.
Antibody TypeMonoclonal Antibody
Entrez Gene Number
Gene Symbol
  • ANO5
  • GDD1
  • TMEM16E
Purification MethodUnpurified
UniProt Number
Molecular Weight~37 kDa observed. ~37 kDa observed. Although the full-length protein has a calculated molecular weight of 107.2 kDa (Uniprot Q75V66), only a ~37 kDa band corresponding to a short spliced variant can be detected by Western blotting when using muscle tissue lysates. A band corresponding to the full-size protein and a 55 kDa band can be detected in addition to the 37 kDa band when using lysates prepared from human embryonal rhabdomyosarcoma (RMS) RD cells, C2C12 murine myoblasts, and fibroblasts.
Physicochemical Information
Dimensions
Materials Information
Toxicological Information
Safety Information according to GHS
Safety Information
Product Usage Statements
Quality AssuranceEvaluated by Western Blotting in mouse skeletal muscle tissue lysate.

Western Blotting Analysis: A 1:500 dilution of this antibody detected Anoctamin-5 in 10 µg of mouse skeletal muscle tissue lysate.
Usage Statement
  • Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
Storage and Shipping Information
Storage ConditionsStable for 1 year at -20°C from date of receipt.
Handling Recommendations: Upon receipt and prior to removing the cap, centrifuge the vial and gently mix the solution. Aliquot into microcentrifuge tubes and store at -20°C. Avoid repeated freeze/thaw cycles, which may damage IgG and affect product performance.
Packaging Information
Material Size200 µL
Transport Information
Supplemental Information
Specifications
Global Trade Item Number
Catalogue Number GTIN
MABS501 04055977175462

Documentation

Anti-Anoctamin-5 Antibody, clone 5F7 MSDS

Title

Safety Data Sheet (SDS) 

Anti-Anoctamin-5 Antibody, clone 5F7 Certificates of Analysis

TitleLot Number
Anti-Anoctamin-5, clone 5F7 -Q2595047 Q2595047

Brochure

Title
New Products Flyer- Vol 4 Signaling Feature- NEW! Phosphohistidine Antibody

Technical Info

Title
Characterization of Estrogen Receptor α Phosphorylation Sites in Breast Cancer Tissue Using the SNAP i.d® 2.0 System
White Paper: Further considerations of antibody validation and usage.

Posters

Title
Post-Translational Modifications