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DR1090 Anti-AHR Mouse mAb (3B12)

Anti-AHR Mouse mAb (3B12) MSDS (material safety data sheet) or SDS, CoA and CoQ, dossiers, brochures and other available documents.

Synonyms: Anti-Aryl Hydrocarbon Receptor

DR1090
  
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Overview

Replacement Information

Key Spec Table

Host
M
Description
Overview

This product has been discontinued.





Recognizes the ~100 kDa AHR protein in COLO 320 HSR and HeLa cells.
Catalogue NumberDR1090
Brand Family Calbiochem®
SynonymsAnti-Aryl Hydrocarbon Receptor
Application Data
Detection of human AHR by immunoblotting. Sample: Whole cell lysate from COLO 320 HSR cells. Primary antibody: Anti-AHR Mouse mAb (3B12) (Cat. No. DR1090) (1-5 µg/ml). Detection: chemiluminescence.

Detection of human AHR by immunocytochemistry. Sample: HeLa cells fixed in paraformaldehyde. Primary antibody: Anti-AHR Mouse mAb (3B12) (Cat. No. DR1090) (10 µg/ml). Detection: fluorescence.
References
ReferencesSchults, M,A., et al. 2010. J. Biol. Chem. 285, 14558.
Product Information
FormLiquid
FormulationIn PBS, pH 7.2.
Negative control293T
Positive controlCOLO 320 HSR cells, HeLa cells
PreservativeNone
Applications
Key Applications Enzyme-Linked Immunosorbent Assay
Immunoblotting (Western Blotting)
Immunocytochemistry
Application NotesELISA (see comments)
Immunoblotting (1-5 µg/ml)
Immunocytochemistry (10 µg/ml)
Application CommentsFor ELISA, this antibody can be used as the capture antibody; recommended concentration is 10 ng/ml, 1 µg/well, 100 µl total volume. Variables associated with assay conditions will dictate the proper working dilution.
Biological Information
ImmunogenA recombinant polypeptide corresponding to amino acids of 721-821 human AHR, expressed as a GST fusion protein
Clone3B12
HostMouse
IsotypeIgG₁
Physicochemical Information
Dimensions
Materials Information
Toxicological Information
Safety Information according to GHS
Safety Information
Product Usage Statements
Storage and Shipping Information
Ship Code Blue Ice Only
Toxicity Regulatory Review
Storage -20°C
Avoid freeze/thaw Avoid freeze/thaw
Do not freeze Ok to freeze
Special InstructionsFollowing initial thaw, aliquot and freeze (-20°C).
Packaging Information
Transport Information
Supplemental Information
Specifications
Global Trade Item Number
Catalogue Number GTIN
DR1090 0

Documentation

References

Reference overview
Schults, M,A., et al. 2010. J. Biol. Chem. 285, 14558.
Data Sheet

Note that this data sheet is not lot-specific and is representative of the current specifications for this product. Please consult the vial label and the certificate of analysis for information on specific lots. Also note that shipping conditions may differ from storage conditions.

Revision04-November-2010 RFH
SynonymsAnti-Aryl Hydrocarbon Receptor
ApplicationELISA (see comments)
Immunoblotting (1-5 µg/ml)
Immunocytochemistry (10 µg/ml)
Application Data
Detection of human AHR by immunoblotting. Sample: Whole cell lysate from COLO 320 HSR cells. Primary antibody: Anti-AHR Mouse mAb (3B12) (Cat. No. DR1090) (1-5 µg/ml). Detection: chemiluminescence.

Detection of human AHR by immunocytochemistry. Sample: HeLa cells fixed in paraformaldehyde. Primary antibody: Anti-AHR Mouse mAb (3B12) (Cat. No. DR1090) (10 µg/ml). Detection: fluorescence.
DescriptionPurified mouse monoclonal antibody. Recognizes the ~100 kDa AHR protein.
BackgroundThis protein is a ligand-activated transcription factor involved in the regulation of biological responses to planar aromatic hydrocarbons. This receptor has been shown to regulate xenobiotic-metabolizing enzymes such as cytochrome P450.
HostMouse
ImmunogenA recombinant polypeptide corresponding to amino acids of 721-821 human AHR, expressed as a GST fusion protein
Clone3B12
IsotypeIgG₁
Specieshuman
Positive controlCOLO 320 HSR cells, HeLa cells
Negative control293T
FormLiquid
FormulationIn PBS, pH 7.2.
PreservativeNone
CommentsFor ELISA, this antibody can be used as the capture antibody; recommended concentration is 10 ng/ml, 1 µg/well, 100 µl total volume. Variables associated with assay conditions will dictate the proper working dilution.
Storage Avoid freeze/thaw
-20°C
Do Not Freeze Ok to freeze
Special InstructionsFollowing initial thaw, aliquot and freeze (-20°C).
Toxicity Regulatory Review
ReferencesSchults, M,A., et al. 2010. J. Biol. Chem. 285, 14558.