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MAB1810-I-25UG Anti-ADAMTS-1 Antibody, clone 5D4E11B6

Anti-ADAMTS-1, clone 5D4E11B6, Cat. No. MAB1810-I, is a mouse monoclonal antibody that detects ADAMTS-1 and is tested for use in ELISA, Immunocytochemistry, Immunofluorescence, and Western Blotting.

MAB1810-I-25UG
25 μg  
Purchase on Sigma-Aldrich

Overview

Replacement Information
Description
Catalogue NumberMAB1810-I-25UG
DescriptionAnti-ADAMTS-1 Antibody, clone 5D4E11B6
Alternate Names
  • A disintegrin and metalloproteinase with thrombospondin motifs 1
  • ADAM-TS 1
  • ADAM-TS1
  • METH-1
Background InformationA disintegrin and metalloproteinase with thrombospondin motifs 1 (UniProt: Q9UHI8; also known as ADAM-TS 1, ADAM-TS1, ADAMTS-1, METH-1) is encoded by the ADAMTS1 (also known as KIAA1346, METH1) gene (Gene ID: 9510) in human. ADAM-TS1 is a zinc-binding protein that is synthesized with a signal peptide (aa 1-49) and a propeptide (aa 50-252) that are subsequently cleaved by furin endopeptidase to generate the mature form (aa 253-967). It has a conserved cysteine in the cysteine-switch motif that binds the catalytic zinc ion and inhibits the enzyme. The dissociation of cysteine from the zinc ion upon the activation-peptide release activates the enzyme. It also contains spacer domain (aa 725-849) and the TSO-1 domain that are important for a tight interaction with the extracellular matrix. ADAM-TS1 cleaves aggrecan, a cartilage proteoglycan at Glu1938-Leu1939 site within the chondroitin sulfate attachment domain and is involved in its turnover. ADAMTS1 is implicated in vascularization and development and displays anti- and pro-tumorigenic activities. ADAMTS1 knockout mice exhibit reduced body weight, kidney malformation, and impaired female fertility. (Ref.: Rodriguez-Baena, FJ., et al. (2018). Sci. Rep. 8: Article 13103).
References
Product Information
FormatPurified
PresentationPurified mouse monoclonal antibody IgG in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide.
Quality LevelMQ200
Applications
ApplicationAnti-ADAMTS-1, clone 5D4E11B6, Cat. No. MAB1810-I, is a mouse monoclonal antibody that detects ADAMTS-1 and is tested for use in ELISA, Immunocytochemistry, Immunofluorescence, and Western Blotting.
Key Applications
  • Western Blotting
  • Immunofluorescence
  • ELISA
  • Immunocytochemistry
Application NotesWestern Blotting Analysis: A 1:125 dilution from a representative lot detected ADAMTS-1 in lysates from A431 cells and E16 mouse embryonic brain tissue.

Western Blotting Analysis: A representative lot detected ADAMTS-1 in Western Blotting applications (Meng, T.G., et. al. (2017). Cell Death Dis. 8(6):e2846; Luque, A., et. al. (2003). J Biol. Chem. 278 (26); 23656-65).

Immunocytochemistry Analysis: A 1:250 dilution from a representative lot detected ADAMTS-1 in HeLa cells.

ELISA Analysis: A representative lot detected ADAMTS-1 in ELISA applications (Luque, A., et. al. (2003). J Biol Chem. 278(26); 23656-65).

Immunofluorescence Analysis: A representative lot detected ADAMTS-1 in Immunofluorescence applications (Silva, S.V., et. al. (2016). PLoS One. 11(10):e0165061; Luque, A., et. al. (2003). J Biol Chem. 278(26); 23656-65).

Note: Actual optimal working dilutions must be determined by end user as specimens, and experimental conditions may vary with the end user
Biological Information
ImmunogenMyc-His-tagged full length recombinant human ADAMTS-1.
EpitopeUnknown
Clone5D4E11B6
Concentration0.5 mg/mL. Please refer to guidance on suggested starting dilutions and/or titers per application and sample type.
HostMouse
SpecificityClone 5D4E11B6 is a mouse monoclonal antibody that detects A disintegrin and metalloproteinase with thrombospondin motifs 1 (ADAMTS-1).
IsotypeIgGκ
Species Reactivity
  • Human
  • Mouse
Species Reactivity NoteHuman, Mouse. Predicted to react with Rat based on 100% sequence homology.
Antibody TypeMonoclonal Antibody
Entrez Gene Number
Gene Symbol
  • ADAMTS1
  • KIAA1346
  • METH1
Purification MethodProtein G purified
UniProt Number
Molecular Weight~58 kDa observed; 105.36 kDa calculated for the complete molecule. Uncharacterized bands may be observed in some lysate(s).
Physicochemical Information
Dimensions
Materials Information
Toxicological Information
Safety Information according to GHS
Safety Information
Product Usage Statements
Quality AssuranceEvaluated by Western Blotting in HEK293 cell lysate.

Western Blotting Analysis: A 1:125 dilution of this antibody detected ADAMTS-1 in HEK293 cell lysate.
Usage Statement
  • Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
Storage and Shipping Information
Storage ConditionsStable for 1 year at +2°C to +8°C from date of receipt.
Packaging Information
Material Size25 μg
Transport Information
Supplemental Information
Specifications
Global Trade Item Number
Catalogue Number GTIN
MAB1810-I-25UG 04065265944892