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07-1416 Anti-4E-BP1 Antibody

Anti-4E-BP1 Antibody detects level of 4E-BP1 & has been published & validated for use in WB, IH(P).

Anti-4E-BP1 Antibody MSDS (material safety data sheet) or SDS, CoA and CoQ, dossiers, brochures and other available documents.
Research Category: Signaling Research Sub-Category: PI3K, Akt, & mTOR Signaling Gene Symbol: EIF4EBP1, PHAS-I, MGC4316, 4E-BP1, 4EBP1, BP-1 UniProt Number: Q13541
07-1416
100 µL  
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Key Spec Table

Species ReactivityKey ApplicationsHostFormatAntibody Type
H, R, M, PoWB, IH(P)RbAffinity PurifiedPolyclonal Antibody
Description
Catalogue Number07-1416
Replaces04-321
DescriptionAnti-4E-BP1 Antibody
References
Product Information
FormatAffinity Purified
Control
  • A431 cell lysate
PresentationPurified rabbit polyclonal IgG in buffer containing Tris-citrate/phosphate, pH 7-8, and 0.1% sodium azide.
Quality LevelMQ100
Applications
ApplicationAnti-4E-BP1 Antibody detects level of 4E-BP1 & has been published & validated for use in WB, IH(P).
Key Applications
  • Western Blotting
  • Immunohistochemistry (Paraffin)
Application NotesImmunohistochemisty (paraffin): 4E-BP1 representative staining pattern/morphology in thyroid cancer. Tissue was pretreated with citrate pH 6, antigen retrieval. A previous lot of this antibody was diluted to 1:100, using IHC-Select® Detection with HRP-DAB. Immunoreactivity is seen as staining pattern/morphology in thyroid cancer tissue.

Western Blot: A previous lot of this antibody was tested on multiple lysates. See datasheet.
Biological Information
ImmunogenSynthetic peptide corresponding to a portion of human eIF4E Binding Protein encoded in part by exon 2 and 3.
HostRabbit
SpecificityEukaryotic translation initiation factor 4E binding protein (eIF4E Binding Protein, eIF4EBP1, 4E-BP1, 4EBP1).
IsotypeIgG
Species Reactivity
  • Human
  • Rat
  • Mouse
  • Pig
Antibody TypePolyclonal Antibody
Entrez Gene Number
Entrez Gene SummaryThis gene encodes one member of a family of translation repressor proteins. The protein directly interacts with eukaryotic translation initiation factor 4E (eIF4E), which is a limiting component of the multisubunit complex that recruits 40S ribosomal subunits to the 5' end of mRNAs. Interaction of this protein with eIF4E inhibits complex assembly and represses translation. This protein is phosphorylated in sponse to various signals including UV irradiation and insulin signaling, resulting in its dissociation from eIF4E and activation of mRNA translation.[provided by RefSeq]
Gene Symbol
  • EIF4EBP1
  • PHAS-I
  • MGC4316
  • 4E-BP1
  • 4EBP1
  • BP-1
Purification MethodAffinity Purfied
UniProt Number
UniProt SummaryFUNCTION: Regulates eIF4E activity by preventing its assembly into the eIF4F complex. Mediates the regulation of protein translation by hormones, growth factors and other stimuli that signal through the MAP kinase pathway.
SUBUNIT: Nonphosphorylated EIF4EBP1 competes with EIF4G1/EIF4G3 to interact with EIF4E; insulin stimulated MAP-kinase (MAPK1 and MAPK3) phosphorylation of EIF4EBP1 causes dissociation of the complex allowing EIF4G1/EIF4G3 to bind and consequent initiation of translation. Rapamycin can attenuate insulin stimulation, mediated by FKBPs.
PTM: Phosphorylated on serine and threonine residues in response to insulin, EGF and PDGF. Phosphorylated upon DNA damage, probably by ATM or ATR.
SIMILARITY: Belongs to the eIF4E-binding protein family.
Molecular Weight15-20 kDa
Physicochemical Information
Dimensions
Materials Information
Toxicological Information
Safety Information according to GHS
Safety Information
Product Usage Statements
Quality AssuranceRoutinely evaluated by Western Blot on A431 lysates.

Western Blot Analysis:
1:500-1:1,000 dilution of this lot detected 4E-BP1 on 10 μg of A431 lysates.
Usage Statement
  • Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
Storage and Shipping Information
Storage ConditionsStable for 6 months at 2-8°C in undiluted aliquots from date of receipt.
Packaging Information
Material Size100 µL
Transport Information
Supplemental Information
Specifications
Global Trade Item Number
Catalogue Number GTIN
07-1416 04053252507229