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176900 Anthrax Lethal Factor, Recombinant, Bacillus anthracis, B. anthracis - Calbiochem

Anthrax Lethal Factor, Recombinant, Bacillus anthracis, B. anthracis - Calbiochem MSDS (material safety data sheet) or SDS, CoA and CoQ, dossiers, brochures and other available documents.
176900
  
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Overview

Replacement Information
Description
Overview

This product has been discontinued.



Recombinant, Bacillus anthracis anthrax lethal factor expressed in a specialized strain of B. anthracis. One of the three protein components of Anthrax toxin, lethal factor (LF) is a highly specific protease that cleaves members of the mitogen-activated protein kinase kinase (MAPKK) family. LF is comprised of four domains. Domain I binds the protective antigen to enter the target cell; domains II, III, and IV create a long groove to hold and cleave the MAPKK proteins.
Catalogue Number176900
Brand Family Calbiochem®
References
ReferencesBrossier, F., and Mock, M. 2001. Toxicon 39, 1747.
Friedlander, A.M. 2001. Nature 414, 160.
Gupta, P., et al. 2001. Biochem. Biophys. Res. Commun. 284, 568.
Pannifer, A.D., et al. 2001. Nature 414, 160.
Pugsley, A.P. 1996. Proc. Natl. Acad. Sci. USA 93, 8155.
Leppla, S.H. 1991. Methods Enzymol. 195, 153.
Product Information
Unit of DefinitionOne unit is defined as the amount of enzyme required to catalyze the release of 1.0 pmole of cleaved MAPKKide™ substrate per min at 37°C, in 20 mM HEPES, pH 8.2.
FormLyophilized
FormulationLyophilized from 50 mM NaCl, 5 mM HEPES, pH 7.5.
Quality LevelMQ100
Applications
Biological Information
Primary TargetMAPKK
PuritySingle major band by SDS-PAGE
Physicochemical Information
Dimensions
Materials Information
Toxicological Information
Safety Information according to GHS
Safety Information
Product Usage Statements
Storage and Shipping Information
Ship Code Ambient Temperature Only
Toxicity Standard Handling
Storage +2°C to +8°C
Do not freeze Ok to freeze
Special InstructionsFollowing reconstitution aliquot and freeze (-20°C). Avoid freeze/thaw cycles of solutions. Stock solutions are stable for up to 6 months at -20°C.
End use certificateY
Canadian export regulations Due to the country and/or U.S. state of origin of the animal material used in this product, this product may not be exported to Canada.
Packaging Information
Transport Information
Supplemental Information
Specifications
Global Trade Item Number
Catalogue Number GTIN
176900 0

Documentation

Anthrax Lethal Factor, Recombinant, Bacillus anthracis, B. anthracis - Calbiochem MSDS

Title

Safety Data Sheet (SDS) 

Anthrax Lethal Factor, Recombinant, Bacillus anthracis, B. anthracis - Calbiochem Certificates of Analysis

TitleLot Number
176900

References

Reference overview
Brossier, F., and Mock, M. 2001. Toxicon 39, 1747.
Friedlander, A.M. 2001. Nature 414, 160.
Gupta, P., et al. 2001. Biochem. Biophys. Res. Commun. 284, 568.
Pannifer, A.D., et al. 2001. Nature 414, 160.
Pugsley, A.P. 1996. Proc. Natl. Acad. Sci. USA 93, 8155.
Leppla, S.H. 1991. Methods Enzymol. 195, 153.
Data Sheet

Note that this data sheet is not lot-specific and is representative of the current specifications for this product. Please consult the vial label and the certificate of analysis for information on specific lots. Also note that shipping conditions may differ from storage conditions.

Revision14-October-2013 JSW
DescriptionRecombinant, Bacillus anthracis anthrax lethal factor expressed in a special strain of B. anthracis. One of three protein components of anthrax toxin produced by the pathogenic bacterium, Bacillus anthracis. Highly specific protease component that cleaves members of the mitogen-activated protein kinase kinase (MAPKK) family. LF is comprised of four domains: domain I that binds to protective antigen (necessary for entry into the cellular target) and domains II, III, and IV which form a long groove to hold and cleave the target proteins. Requires passage through an acidic vesicle prior to translocation to the cytoplasm of the cell. Cleavage of target proteins leads to the inhibition of multiple signaling pathways and cell death.
FormLyophilized
FormulationLyophilized from 50 mM NaCl, 5 mM HEPES, pH 7.5.
PuritySingle major band by SDS-PAGE
Unit definitionOne unit is defined as the amount of enzyme required to catalyze the release of 1.0 pmole of cleaved MAPKKide™ substrate per min at 37°C, in 20 mM HEPES, pH 8.2.
SolubilityPlease refer to the vial label for lot-specific reconstitution volume.
Storage +2°C to +8°C
Do Not Freeze Ok to freeze
Special InstructionsFollowing reconstitution aliquot and freeze (-20°C). Avoid freeze/thaw cycles of solutions. Stock solutions are stable for up to 6 months at -20°C.
Toxicity Standard Handling
ReferencesBrossier, F., and Mock, M. 2001. Toxicon 39, 1747.
Friedlander, A.M. 2001. Nature 414, 160.
Gupta, P., et al. 2001. Biochem. Biophys. Res. Commun. 284, 568.
Pannifer, A.D., et al. 2001. Nature 414, 160.
Pugsley, A.P. 1996. Proc. Natl. Acad. Sci. USA 93, 8155.
Leppla, S.H. 1991. Methods Enzymol. 195, 153.