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176904 Anthrax Lethal Factor Protease Substrate III, Fluorogenic - Calbiochem

An N-acetylated, C-7-amido-4-methylcoumarin (AMC) derivative of a 14-mer peptide substrate designed from the MEK-2 template that is useful for measuring Anthrax lethal factor (LF) metalloproteolytic activity with a detection limit of ~5-10 pM.

Anthrax Lethal Factor Protease Substrate III, Fluorogenic - Calbiochem MSDS (material safety data sheet) or SDS, CoA and CoQ, dossiers, brochures and other available documents.

Synonyms: Anthrax LF Protease Substrate III, Fluorogenic, Bacillus anthracis LF Protease Substrate III, Fluorogenic

176904
  
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Overview

Replacement Information

Key Spec Table

Empirical Formula
C₉₁H₁₅₆N₄₂O₁₈
Description
Overview

This product has been discontinued.



An N-acetylated, C-7-amido-4-methylcoumarin (AMC) derivative of a 14-mer peptide substrate designed from the MEK-2 template that is useful for measuring Anthrax lethal factor (LF) metalloproteolytic activity with a detection limit of ~5-10 pM. Reported to be useful for high-throughput screening of LF inhibitors.
Catalogue Number176904
Brand Family Calbiochem®
SynonymsAnthrax LF Protease Substrate III, Fluorogenic, Bacillus anthracis LF Protease Substrate III, Fluorogenic
References
ReferencesTonello, F., et. al. 2002. Nature 418, 386.
Product Information
ATP CompetitiveN
FormLyophilized
FormulationSupplied as a trifluoroacetate salt.
Hill FormulaC₉₁H₁₅₆N₄₂O₁₈
Chemical formulaC₉₁H₁₅₆N₄₂O₁₈
Hygroscopic Hygroscopic
ReversibleN
Quality LevelMQ100
Applications
Biological Information
Primary TargetMeasuring Anthrax lethal factor (LF) metalloproteolytic activity
Purity≥97% by HPLC
Physicochemical Information
Cell permeableN
Emission max.
Excitation max.
Peptide SequenceAc-Gly-Tyr-βAla-Arg-Arg-Arg-Arg-Arg-Arg-Arg-Arg-Val-Leu-Arg-AMC
Dimensions
Materials Information
Toxicological Information
Safety Information according to GHS
Safety Information
Product Usage Statements
Storage and Shipping Information
Ship Code Blue Ice Only
Toxicity Standard Handling
Storage -20°C
Protect from Light Protect from light
Hygroscopic Hygroscopic
Do not freeze Ok to freeze
Special InstructionsFollowing reconstitution aliquot and freeze (-20°C). Stock solutions are stable for up to 3 months at -20°C.
Packaging Information
Packaged under inert gas Packaged under inert gas
Transport Information
Supplemental Information
Specifications
Global Trade Item Number
Catalogue Number GTIN
176904 0

Documentation

Anthrax Lethal Factor Protease Substrate III, Fluorogenic - Calbiochem MSDS

Title

Safety Data Sheet (SDS) 

Anthrax Lethal Factor Protease Substrate III, Fluorogenic - Calbiochem Certificates of Analysis

TitleLot Number
176904

References

Reference overview
Tonello, F., et. al. 2002. Nature 418, 386.
Data Sheet

Note that this data sheet is not lot-specific and is representative of the current specifications for this product. Please consult the vial label and the certificate of analysis for information on specific lots. Also note that shipping conditions may differ from storage conditions.

Revision15-April-2008 RFH
SynonymsAnthrax LF Protease Substrate III, Fluorogenic, Bacillus anthracis LF Protease Substrate III, Fluorogenic
DescriptionAn N-acetylated, C-7-amido-4-methylcoumarin (AMC) derivative of a 14-mer peptide substrate designed from the MEK-2 template that is useful for measuring Anthrax Lethal Factor (LF) metalloproteolytic activity with a detection limit of ~5-10 pM. Reported to be useful for high-throughput screening of LF inhibitors. Excitation max.: ~360 nm; emission max.: ~460 nm.
FormLyophilized
FormulationSupplied as a trifluoroacetate salt.
Intert gas (Yes/No) Packaged under inert gas
Chemical formulaC₉₁H₁₅₆N₄₂O₁₈
Peptide SequenceAc-Gly-Tyr-βAla-Arg-Arg-Arg-Arg-Arg-Arg-Arg-Arg-Val-Leu-Arg-AMC
Purity≥97% by HPLC
SolubilityDMSO (5 mg/ml), 5% Acetic Acid (5 mg/ml), or H₂O (1 mg/ml)
Storage Protect from light
-20°C
Hygroscopic
Do Not Freeze Ok to freeze
Special InstructionsFollowing reconstitution aliquot and freeze (-20°C). Stock solutions are stable for up to 3 months at -20°C.
Toxicity Standard Handling
ReferencesTonello, F., et. al. 2002. Nature 418, 386.