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SCR005 Accutase cell detachment solution

A cell detachment solution of proteolytic & collagenolytic enzymes. The reagent is useful for creating single cell suspensions from clumped cell cultures for accurate cell counting, detachment of cells from primary tissue.

Accutase cell detachment solution MSDS (material safety data sheet) or SDS, CoA and CoQ, dossiers, brochures and other available documents.
SCR005
100 mL  
Purchase on Sigma-Aldrich

Overview

Replacement Information
Description
Catalogue NumberSCR005
Brand Family Chemicon®
Trade Name
  • Chemicon
DescriptionAccutase cell detachment solution
OverviewA cell detachment solution of proteolytic and collagenolytic enzymes. Useful for the routine detachment of cells from standard tissue culture plasticware and adhesion coated plasticware, including SmartPlastic™. ACCUTASE™ does not contain mammalian or bacterial derived products.

Because the reagent does not contain universally proteolytic enzymes it may not release all cell/tissue types uniformly. Some optimization of cell type and passage number, as well as, incubation time and reagent strength typically needs to be performed to optimize the reagents effectiveness.

ACCUTASE™ has been shown to be effective on a wide variety of cell types including: fibroblasts, keratinocytes, vascular endothelial cells, hepatocytes, vascular smooth muscle cells, hepatocyte progenitors, primary chick embryo neuronal cells, bone marrow stem cells, adherent CHO and BHK cells, macrophages, 293 cells, L929 cells, immortalized mouse testicular germ cells, 3T3, Vero, COS, HeLa, NT2, MG63, M24 and A375 metastatic melanoma, gliomas U251 and D54, HT1080 fibrosarcoma cells, and Sf9 insect cells.

Each lot of ACCUTASE™ is tested for Sterility (by USP membrane filtration method), enzymatic activity (tested with synthetic chromagenic tetrapeptides) and cell detachment from tissue culture plastic.
References
Product Information
HS Code3507 90 49
Presentation100 ml, ready to use, frozen sterile filtered (0.2 micron) liquid. 1X ACCUTASE enzymes in Dulbecco's PBS (0.2 g/L KCl, 0.2 g/L KH2PO4, 8 g/L NaCl, and 1.15 g/L Na2HPO4) containing 0.5 mM EDTA·4Na and 3 mg/L Phenol Red.
Quality LevelMQ300
Applications
ApplicationA cell detachment solution of proteolytic & collagenolytic enzymes. The reagent is useful for creating single cell suspensions from clumped cell cultures for accurate cell counting, detachment of cells from primary tissue.
Application NotesCell Detachment

1. Thaw ACCUTASE™ at room temperature.

2. Wash plate, flask or beads with sterile PBS.

3. Add ACCUTASE™ to culture dish or flask using aseptic procedures at 10 ml per 75-cm2 surface area.

4. Return culture to 37°C incubator and allow cells to detach (5-10 minutes).

5. Count cells and passage as usual. No additional washes or enzyme inhibitors are required



Optimal incubation times and reagent strength needs to be determined for each cell/tissue type tested by the enduser.
Biological Information
Media FormLiquid
Stem Cell Type
  • Mouse Embryonic Stem Cells
  • Human Embryonic Stem Cells
  • Mesenchymal Stem Cells
  • Neural Stem Cells
  • Hematopoietic Stem Cells
  • Epithelial Cells
  • Pancreatic Stem Cells
  • Cardiac Stem Cells
  • Induced Pluripotent Stem Cells
Physicochemical Information
Dimensions
Materials Information
Toxicological Information
Safety Information according to GHS
Safety Information
Product Usage Statements
Usage Statement
  • Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
Storage and Shipping Information
Storage ConditionsStable when stored at -20°C. Refer to lot expiration date. Recommended storage upon receipt is -20°C. After thawing, ACCUTASE may be stored for up to 2 months at 4°C. DO NOT STORE AT ROOM TEMP
Packaging Information
Material Size100 mL
Transport Information
Supplemental Information
Specifications
Global Trade Item Number
Catalogue Number GTIN
SCR005 04053252267819

Documentation

Accutase cell detachment solution MSDS

Title

Safety Data Sheet (SDS) 

Accutase cell detachment solution Certificates of Analysis

TitleLot Number
ACCUTASE SOLUTION - 1970316 1970316
ACCUTASE SOLUTION - 2000796 2000796
ACCUTASE SOLUTION - 2022641 2022641
ACCUTASE SOLUTION - 2058324 2058324
ACCUTASE SOLUTION - 2092639 2092639
ACCUTASE SOLUTION - 2138544 2138544
ACCUTASE SOLUTION - 2197143 2197143
ACCUTASE™ SOLUTION 3047970
ACCUTASE™ SOLUTION 2943256
ACCUTASE™ SOLUTION 3062023
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References

Reference overviewPub Med ID
HDAC1 regulates pluripotency and lineage specific transcriptional networks in embryonic and trophoblast stem cells.
Kidder B. L. & Palmer S.
Nucleic Acids Res.  1-15  2010

Show Abstract 		22156375 22156375

Technical Info

Title
Derivation of oligodendrocyte progenitor cells from a human neural stem cell line

Data Sheet

Title
ACCUTASE™ SOLUTION
Reprogramming Cell Fate and Function Novel Strategies for iPSC Generation, Characterization, and Differentiation

FAQ

QuestionAnswer
What is the difference between Accuma and Accutase?Accumax (Catalog #SCR006) will dissociate clumped cells in minutes resulting in a single cell suspension. It will yield accurate reproducible cell counts, is cost effective and is ready to use. The Accutase (Catalog #SCR005) contains phenol red. It will detect adherent cells within minutes and will dissociate tissues for primary cell culture use. It also will yield accurate reproducible cell counts, is cost effective and is ready to use.

Newsletters / Publications

Title
Cellutions - The newsletter for Cell Biology Researchers Volume 3: 2011