Millipore Sigma Vibrant Logo
Attention: We have moved. Merck Millipore products are no longer available for purchase on MerckMillipore.com.Learn More

108512 AFB-Color carbol fuchsin riešenie

108512
Purchase on Sigma-Aldrich

Overview

Replacement Information

Products

Catalogue NumberPackaging Qty/Pack
1085120500 Plastová flaša 500 ml
1085122500 Plastová flaša 2.5 l
Description
Catalogue Number108512
DescriptionAFB-Color carbol fuchsin solution
OverviewThe AFB-Color carbol fuchsin solution - for the microscopic investigation of acid-fast bacteria (AFB) (cold staining), is a ready-to- use solution used for human-medical cell diagnosis and serves the purpose of the bacteriological and histological investigation of sample material of human origin, for example smears of enriched bacterial cultures or histological sections of e. g. the lung. The cell wall of acid-fast bacteria has a high proportion of wax and lipids and hence absorbs dyes only very slowly. The most efficient staining method is the hot staining according to Ziehl-Neelsen. In this method, carbolfuchsin solution is applied to the specimen and heated. This heating process accelerates the rate at which the fuchsin dye is absorbed and thus the formation of the mycolate-fuchsin complex in the cell wall. The AFB-Color Kit (Cat. no. 116450) offers a modified AFB-Color Carbol fuchsin solution which abolishes heating (Kinyoun method, cold staining). Once the acid-fast bacteria have absorbed the fuchsin dye, it is virtually impossible to decolorize them again, even after intense treatment with a decolorizing solution such as e. g. hydrochloric acid in ethanol. Accordingly, acid-fast bacteria are termed as acid- and alcohol-fast for staining, and are stained red in the microscopic visualization. Correspondingly, all non-acid-fast microorganisms are counterstained with an appropriate dye. In this instruction for use 110630 AFB-Color malachite green (oxalate) solution or 101287 Loeffler's methylene blue is used for counterstaining.
The 500 ml bottle of AFB-Color carbol fuchsin provides approx. 250 stainings.
Pretreatment of the specimens with Sputofluol®(Cat. No. 108000) dissolves the bacteria from the surrounding viscid sputum and cell material. This product is registered as IVD and CE marked. For more details, please see instructions for use (IFU). The IFU can be downloaded from this webpage.
References
Product Information
HS Code3212 90 90
Quality LevelMQ400
Applications
Applicationfor the microscopic investigation of acid-fast bacteria (AFB) (cold staining)
Biological Information
Physicochemical Information
Density0.99 g/cm3 (20 °C)
Flash point48 °C
pH value3.6 (H₂O, 20 °C)
Dimensions
Materials Information
Toxicological Information
Safety Information according to GHS
Hazard Pictogram(s) Hazard Pictogram(s)
Hazard Pictogram(s)
Hazard Pictogram(s)
Hazard Pictogram(s)
Hazard Statement(s)H226: Horľavá kvapalina a pary.
H314: Spôsobuje vážne poleptanie kože a poškodenie očí.
H341: Podozrenie, že spôsobuje genetické poškodenie.
H410: Veľmi toxický pre vodné organizmy, s dlhodobými účinkami.
Precautionary Statement(s)P210: Uchovávajte mimo dosahu tepla, horúcich povrchov, iskier, otvoreného ohňa a iných zdrojov zapálenia. Nefajčite.
P273: Zabráňte uvoľneniu do životného prostredia.
P280: Použite ochranné rukavice/ ochranný odev/ ochranu očí/ ochranu tváre/ ochranu sluchu.
P303 + P361 + P353: PRI KONTAKTE S POKOŽKOU (alebo vlasmi): Vyzlečte všetky kontaminované časti odevu. Pokožku ihneď opláchnite vodou.
P304 + P340 + P310: PO VDÝCHNUTÍ: Presuňte osobu na čerstvý vzduch a umožnite jej pohodlne dýchať. Okamžite volajte TOXIKOLOGICKÉ INFORMAČNÉ CENTRUM/lekára.
P305 + P351 + P338: PO ZASIAHNUTÍ OČÍ: Niekoľko minút ich opatrne vyplachujte vodou. Ak používate kontaktné šošovky a je to možné, odstráňte ich. Pokračujte vo vyplachovaní.
Signal WordNebezpečenstvo
Storage class3 Vznietlivé kvapaliny
WGKWGK 3 silne ohrozujúce vody
Disposal9
Karcinogénne zlúčeniny a horľavé zlúčeniny označené ako "veľmi jedovaté" alebo "jedovaté": nádoba F. Alkylsulfáty sú karcinogénne. Buďte osobitne opatrní, aby nedošlo k vdýchnutiu alebo ku kontaktu s pokožkou. Alkylsulfáty neutralizujte pridaním (z oddeľovacieho lievika) koncentrovaného ľadového roztoku amoniaku (kat. č. 105426) po kvapkách za intenzívneho miešania. Pred vyliatím do nádoby D skontrolujte pH pomocou indikátorových prúžkov pH-Universal (kat. č. 109535).
Safety Information
Categories of dangerhor?avá, škodlivá, žieravá, mutagénna
Product Usage Statements
Storage and Shipping Information
StorageSkladujte pri +15°C až +25°C.
Packaging Information
Transport Information
Declaration (railroad and road) ADR, RIDUN 1992 , 3 (6.1), III
Declaration (transport by air) IATA-DGRUN 1992 , 3 (6.1), III
Declaration (transport by sea) IMDG-CodeUN 1992 , 3 (6.1), III
Supplemental Information
Specifications
Global Trade Item Number
Catalogue Number GTIN
1085120500 04022536105888
1085122500 04022536557175