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194437 ABTS™ Chromophore, Diammonium Salt

ABTS™ Chromophore, Diammonium Salt MSDS (material safety data sheet) or SDS, CoA and CoQ, dossiers, brochures and other available documents.
194437
  
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Overview

Replacement Information
Description
OverviewContains 20 tablets. Each tablet contains 5 mg and makes 5 ml of ABTS™ Chromophore working substrate for ELISA.
Catalogue Number194437
Brand Family Calbiochem®
References
Product Information
CAS number30931-67-0
FormSolid
Hill FormulaC₁₈H₁₈N₄O₆S₄ · (NH₃)₂
Chemical formulaC₁₈H₁₈N₄O₆S₄ · (NH₃)₂
Quality LevelMQ100
Applications
Biological Information
Physicochemical Information
Dimensions
Materials Information
Toxicological Information
Safety Information according to GHS
RTECSDL7002000
Safety Information
R PhraseR: 36/37/38

Irritating to eyes, respiratory system and skin.
S PhraseS: 26-36

In case of contact with eyes, rinse immediately with plenty of water and seek medical advice.
Wear suitable protective clothing.
Product Usage Statements
Storage and Shipping Information
Ship Code Ambient Temperature Only
Toxicity Irritant
Storage +2°C to +8°C
Protect from Light Protect from light
Do not freeze Ok to freeze
Special InstructionsUnstable in solution; reconstitute just prior to use and discard any unused reconstituted substrate.
Packaging Information
Transport Information
Supplemental Information
Specifications
Global Trade Item Number
Catalogue Number GTIN
194437 0

Documentation

ABTS™ Chromophore, Diammonium Salt MSDS

Title

Safety Data Sheet (SDS) 

ABTS™ Chromophore, Diammonium Salt Certificates of Analysis

TitleLot Number
194437
Data Sheet

Note that this data sheet is not lot-specific and is representative of the current specifications for this product. Please consult the vial label and the certificate of analysis for information on specific lots. Also note that shipping conditions may differ from storage conditions.

Revision06-March-2009 JSW
DescriptionUseful as a substrate for ELISA; acts as a hydrogen donor in peroxidase reactions. Radical cation has an absorption maximum at 414 nm.
FormSolid
Recommended reaction conditions
For Use as ELISA Substrate Note: Each tablet contains 5 mg and yields 5 ml ABTS® working substrate for ELISA when reconstituted as outlined below. 1. Dissolve ABTS at a final conc. of 1 mg/ml in 0.1 M sodium citrate buffer at pH 4.2. 2. Add 1.8 µl 30% H2O2 to 6 ml of the 1 mg/ml ABTS solution above (or 3 µl 3% H2O2 to 1 ml of the ABTS solution). 3. Add 1 ng HRP-conjugated antibody or HRP to 1 ml of the ABTS solution containing H2O2. 4. Incubate for 30 min to 1 h at 37°C (room temp is also OK, but the reaction is faster at 37°C). 5. Stop the reaction at the end of incubation with 1 ml (i.e. equal volume) of stop solution (0.625 M oxalic acid) and read the absorbance at 405 nm.
CAS number30931-67-0
RTECSDL7002000
Chemical formulaC₁₈H₁₈N₄O₆S₄ · (NH₃)₂
Solubility0.1 M sodium citrate buffer, pH 4.2 (1 mg/ml)
Storage Protect from light
+2°C to +8°C
Do Not Freeze Ok to freeze
Special InstructionsUnstable in solution; reconstitute just prior to use and discard any unused reconstituted substrate.
Toxicity Irritant