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480706 α2,3-Neuraminidase, Macrobdella decora, Recombinant, E. coli

α2,3-Neuraminidase, Macrobdella decora, Recombinant, E. coli MSDS (material safety data sheet) or SDS, CoA and CoQ, dossiers, brochures and other available documents.

Synonyms: Sialidase L

480706
  
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Overview

Replacement Information
Description
Overview

This product has been discontinued.



Recombinant, Macrobdella decora α2,3-Neuraminidase expressed in E. coli. Catalyzes the hydrolysis of α2,3-linked N-acetylneuraminic acid from complex oligosaccharides. Will catalyze the cleavage of α2,3-linkages from α1-acid glycoprotein, fetuin, keratan sulfate, sialyl Lewis x, and 3′-sialyllactose, yielding 2,7-anhydro-Neu5Ac instead of “free Neu5Ac” as the hydrolysis product.
Note: 1 mU = 1 milliunit.
Catalogue Number480706
Brand Family Calbiochem®
SynonymsSialidase L
References
ReferencesChou, M.-Y., et al. 1996. J. Biol. Chem. 271, 19219.
Product Information
CAS number9001-67-6
Unit of DefinitionOne unit is defined as the amount of enzyme that will catalyze the release of 1 nmol methylumbelliferone from 2ʹ-(4-Methylumbelliferyl)-D-N-acetylneuraminic Acid (Cat. No. 474495) per min at 37°C, pH 5.5.
EC number3.2.1.18
FormLiquid
FormulationIn 10 mM phosphate buffer, 2% glycerol, pH 7.0.
Quality LevelMQ100
Applications
Biological Information
Specific Activity≥2000 U/mg protein
Concentration Label Please refer to vial label for lot-specific concentration
Physicochemical Information
ContaminantsCeramide glycanase, α- and β-galactosidase, β-hexosaminidase, α- and β-mannosidase, proteases: none detected
Dimensions
Materials Information
Toxicological Information
Safety Information according to GHS
RTECSQQ3450000
Safety Information
R PhraseR: 20-36/37/38-42/43

Harmful by inhalation.
Irritating to eyes, respiratory system and skin.
May cause sensitization by inhalation and skin contact.
S PhraseS: 26-36

In case of contact with eyes, rinse immediately with plenty of water and seek medical advice.
Wear suitable protective clothing.
Product Usage Statements
Storage and Shipping Information
Ship Code Blue Ice Only
Toxicity Harmful
Storage +2°C to +8°C
Do not freeze Ok to freeze
Packaging Information
Transport Information
Supplemental Information
Specifications
Global Trade Item Number
Catalogue Number GTIN
480706 0

Documentation

α2,3-Neuraminidase, Macrobdella decora, Recombinant, E. coli Certificates of Analysis

TitleLot Number
480706

References

Reference overview
Chou, M.-Y., et al. 1996. J. Biol. Chem. 271, 19219.
Data Sheet

Note that this data sheet is not lot-specific and is representative of the current specifications for this product. Please consult the vial label and the certificate of analysis for information on specific lots. Also note that shipping conditions may differ from storage conditions.

Revision01-June-2009 JSW
SynonymsSialidase L
DescriptionRecombinant, Macrobdella decora α2,3-Neuraminidase expressed in E. coli. Catalyzes the hydrolysis of α2,3-linked N-acetylneuraminic acid from complex oligosaccharides. Will catalyze the cleavage of α2,3-linkages from α1-acid glycoprotein, fetuin, keratan sulfate, sialyl Lewis x, and 3'-sialyllactose, yielding 2,7-anhydro-Neu5Ac instead of "free Neu5Ac" as the hydrolysis product.
FormLiquid
FormulationIn 10 mM phosphate buffer, 2% glycerol, pH 7.0.
Concentration Label Please refer to vial label for lot-specific concentration
CAS number9001-67-6
RTECSQQ3450000
EC number3.2.1.18
ContaminantsCeramide glycanase, α- and β-galactosidase, β-hexosaminidase, α- and β-mannosidase, proteases: none detected
Specific activity≥2000 U/mg protein
Unit definitionOne unit is defined as the amount of enzyme that will catalyze the release of 1 nmol methylumbelliferone from 2ʹ-(4-Methylumbelliferyl)-D-N-acetylneuraminic Acid (Cat. No. 474495) per min at 37°C, pH 5.5.
Storage +2°C to +8°C
Do Not Freeze Ok to freeze
Toxicity Harmful
ReferencesChou, M.-Y., et al. 1996. J. Biol. Chem. 271, 19219.