Our broad portfolio consists of multiplex panels that allow you to choose, within the panel, analytes that best meet your needs. On a separate tab you can choose the premixed cytokine format or a single plex kit.
Cell Signaling Kits & MAPmates™
Choose fixed kits that allow you to explore entire pathways or processes. Or design your own kits by choosing single plex MAPmates™, following the provided guidelines.
The following MAPmates™ should not be plexed together:
-MAPmates™ that require a different assay buffer
-Phospho-specific and total MAPmate™ pairs, e.g. total GSK3β and GSK3β (Ser 9)
-PanTyr and site-specific MAPmates™, e.g. Phospho-EGF Receptor and phospho-STAT1 (Tyr701)
-More than 1 phospho-MAPmate™ for a single target (Akt, STAT3)
-GAPDH and β-Tubulin cannot be plexed with kits or MAPmates™ containing panTyr
.
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Select A Species, Panel Type, Kit or Sample Type
To begin designing your MILLIPLEX® MAP kit select a species, a panel type or kit of interest.
Custom Premix Selecting "Custom Premix" option means that all of the beads you have chosen will be premixed in manufacturing before the kit is sent to you.
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96-Well Plate
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Add Additional Reagents (Buffer and Detection Kit is required for use with MAPmates)
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48-602MAG
Buffer Detection Kit for Magnetic Beads
1 Kit
Space Saver Option Customers purchasing multiple kits may choose to save storage space by eliminating the kit packaging and receiving their multiplex assay components in plastic bags for more compact storage.
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Analysis of protein and lipid content from a single sample measurement using the Direct Detect® spectrometer. Because the sample composition was unknown, analysis of the phospholipids was performed using the “Relative Absorbance” mode. (click to enlarge)
Simplified Analysis of Lipid or Detergent Content in Biological Samples Using the IR-Based Direct Detect® Spectrometer
Lipids account for the major (~50%) compositional and structural element of biological membranes, and are present in many cell-derived biological samples, lysates and/or protein preparations. The Direct Detect® spectrometer enables simultaneous protein quantitation and lipid analysis, in the same sample. This method can be used to monitor the efficiency of detergent removal during preparation of samples for downstream analysis and for quantitation of known lipid(s) in cases where a viable standard curve has been determined.
Traditional Techniques for Lipid Characterization: Disadvantages
Lipid content as determined using the Direct Detect® spectrometer in the “Relative Absorbance” mode in 3 different fractions of breast cancer tissue lysates prepared with CytoBuster™ Protein Extraction Reagent or RIPA buffer. (click to enlarge)
Analytical characterization of lipids typically requires that samples undergo laborious, multistep preparative processes prior to analysis. Lipids can be isolated by liquid–liquid extraction and separated into classes, often derivatized, and then analyzed. Conventional methods of analysis include measuring iodine value, elemental phosphorus, acid value (saponification equivalent), peroxide value and radiochemical techniques. More recently, classical methods have been replaced by thin-layer chromatography (TLC), gas chromatography (GC), and high performance liquid chromatography (HPLC) as well as mass spectrometry (MS).
Benefits of Using Direct Detect® Spectrometer for Lipid or Detergent Characterization
The Direct Detect® spectrometer uses the C-H symmetric stretching vibrational population between 2870 and 2840 cm-1 to determine lipid or detergent content in a single step, with minimal sample preparation required. The Direct Detect® assay-free sample card enables analysis of aqueous-based biological samples, which are normally not compatible with infrared spectroscopy, due to their high water content. These assay-free cards are also compatible with many organic solvents. Given that each lipid possesses an IR signature uniquely defined by its chemical composition and structure, IR spectroscopy further offers a means of qualitative lipid discrimination.
Monitoring Lipid Profile During Lysate Preparation
Direct Detect® spectrometer has enabled rapid analysis of total protein with simultaneous monitoring of lipid content, offering a means for in-line process optimization for maximal yield and/or purity, and thereby simplifying and improving downstream analysis.
