| Live Cell Imaging of Apoptosis in Immune Cells using the CellASIC® ONIX2 Microfluidic System |
Live Cell Imaging of Hypoxia in Cancer Cells using the CellASIC® ONIX2 Microfluidic System |
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Jurkat cells (88042803) were first loaded onto the CellASIC® ONIX2 System M04T Pad Trap Plate (M04T-01-5PK) and were then incubated with BioTracker NucView® 488 Green Caspase-3 Dye (SCT101) and Annexin V Texas Red (Red) for the first 2 hours in complete RPMI-1640 media (SLM-240-B), followed by treatment of 2.5 µg/mL CD-95 antibody (05-201) (experiment group) or no treatment (control) for 8 hours. Time-lapse imaging was taken on cells at a 10-minute interval under 400x magnification using a FITC and Texas-Red filter. Read the Application Note
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A431 epidermal carcinoma cells (85090402) were incubated 16 hours overnight with 3µM BioTracker 520 Green Hypoxia Dye (SCT033) in complete DMEM by gravity perfusion at 37°C under normoxic conditions (20% O2) using the CellASIC® ONIX2 System. After overnight incubation, cells were perfused with complete DMEM media without dye at 1 psi under hypoxic conditions (0% O2) for 16 hours at 37°C. Fluorescent images were captured over 16 hours using 465 nm excitation, and a 525 nm emission filter. The BioTracker 520 Green Hypoxia Dye increases in fluorescence intensity with decreasing oxygen levels (increased hypoxic conditions) Read the Application Note
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The CellASIC® ONIX2 Microfluidic System |
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Precision Control of Your Cell Culture Environment for Advanced Live Cell Imaging and Microscopy
Life Is Dynamic. Why study living processes by killing your cells?
Scientists recognize that live cell analysis yields necessary and unique insight into living processes that are rapidly changing. While collecting data from a moment in time with classical endpoint analyses has value, the increasingly common practice of observing and measuring cellular changes over extended periods of time reveals more about the true behavior of biological systems. The challenge has been in creating a dynamic cell culture environment that is controllable, manipulatable and reproducible.There Is Simply No Better Way to Conduct Live Cell Analysis.
The ability to grow, observe and manipulate complex cultures requires precision control over the cell culture environment. The second generation CellASIC® ONIX2 Microfluidic System is a refined, yet powerful automated platform for precise manipulation of multiple key cell culture parameters, enabling measurement of cellular responses to pre-programmed media, temperature and gas environment changes.The CellASIC® ONIX2 Microfludic System uses high-quality, optically clear microfluidic plates and intuitive software, while integrating with a broad range of inverted microscopes to allow continuous, high magnification observation of live cells — as they react to their environment in time.
A New Sophisticated Addition for Your Microscope
Is a lack of precise environmental control causing problems with your experiments? Give your microscope some help and elevate your live cell imaging with the CellASIC® ONIX2 Microfluidic System.- Small control system fits easily next to your microscope.
- Low profile manifold and plate sits on top of your microscope stage delivering precise control of gas, temperature, media changes and more.
- Microfluidic plates with high quality glass bottoms (#1.5) for the best optical quality.
- Talk to a specialist to find out more about how your experiment can benefit from microfluidics.
Microfluidic Technology for Maximum Control
A smaller cell culture environment is easier to control and change. Using the power of microfluidic technology, The CellASIC® ONIX2 System and plates deliver a level of precision that is impossible to achieve with traditional cell culture.- Faster media changes and faster addition/removal of drugs and other stimulants.
- Quickly switch between 2 gas conditions; ideal for studying hypoxic environments.
- Talk to a specialist to find out more about how your experiment can benefit from microfluidics.
- East to use and programmable software for automated hands-free cell culture experiments.
| CellASIC® ONIX2 Microfluidic System and Manifolds | Cat. No. | Qty/Pk |
|---|---|---|
CellASIC® ONIX2 Microfluidic System
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CAX2-S0000 | 1 |
| CellASIC® ONIX2 Manifold XT (Temperature Controlled) | CAX2-MXT20 | 1 |
| CellASIC® ONIX2 Manifold Basic (No Temperature Control) | CAX2-MBC20 | 1 |
| Microfluidic Plates | Cat. No. | Qty/Pk |
|---|---|---|
| CellASIC® ONIX plate for bacteria cells | B04A-03-5PK | 5 |
| CellASIC® ONIX gradient plate for mammalian cells | M04G-02-5PK | 5 |
| CellASIC® ONIX open-top plate for mammalian cells | M04L-03-5PK | 5 |
| CellASIC® ONIX switching plate for mammalian cells | M04S-03-5PK | 5 |
| CellASIC® ONIX Pad Trap Plate, 12µm | M04T-01-5PK | 5 |
| CellASIC® ONIX plate for haploid yeast cells | Y04C-02-5PK | 5 |
| CellASIC® ONIX plate for diploid yeast cells | Y04E-01-5PK | 5 |
| CellASIC® ONIX Pad Trap Plate, 4µm | Y04T-04-5PK | 5 |