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70785 pET Trx Fusion System 32 - Novagen

pET vectors plus host strains with induction control

pET Trx Fusion System 32 - Novagen MSDS (material safety data sheet) or SDS, CoA and CoQ, dossiers, brochures and other available documents.
70785
  
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Replacement Information
Description
Overview

This product has been discontinued.





pET Expression Systems and pET
pET Expression Systems and pET Expression Systems plus Competent Cells provide core reagents needed for target gene cloning and expression.

Components: pET Expression Systems
Components for pET Expression Systems are similar for all systems unless otherwise stated with the specific pET Expression System description. pET Expression Systems include:
• 10 µg pET vector DNA (for each indicated plasmid)
• 0.2 ml BL21 Glycerol Stock
• 0.2 ml BL21(DE3) Glycerol Stock
• 0.2 ml BL21(DE3)pLysS Glycerol Stock
• 0.2 ml Induction Control Glycerol Stock

Components: pET Expression Systems plus Competent Cells
pET Expression Systems plus Competent Cells contain all of the components of the specific pET Expression System, as well as the following additional components, unless otherwise stated with the specific pET Expression System description:
• 0.2 ml NovaBlue Competent Cells
• 0.2 ml BL21(DE3) Competent Cells
• 0.2 ml BL21(DE3)pLysS Competent Cells
• 2 × 0.2 ml SOC Medium
• 10 µl Test Plasmid

These components are sufficient for up to 10 transformations in each host.

Purification and Detection Reagents
Purification and detection reagents are available separately. For complete product descriptions and ordering information, refer to the Protein Purification and Antibodies, Conjugates & Detection Tools chapters.

pET Trx Fusion System 32
The popular pET Trx Fusion System 32 is designed for cloning and high-level expression of polypeptide sequences fused with the 109-aa Trx•Tag™ thioredoxin protein. Many proteins that are normally produced in an insoluble form in E. coli tend to become more soluble when fused with the Trx•Tag sequence (LaVallie 1993, Novy 1995). The pET-32 vectors are compatible with the trxB/gor mutant Origami, Origami 2, Origami B, Rosetta-gami, Rosetta-gami 2, and Rosetta-gami B strains. The thioredoxin reductase (trxB) mutation has been shown to allow the formation of disulfide bonds in the E. coli cytoplasm (Derman 1993). The thioredoxin fusion tag expressed from pET-32 vectors not only enhances the solubility of many target proteins, but appears to catalyze the formation of disulfide bonds in the cytoplasm of trxB mutants (Stewart 1998). The glutathione reductase (gor) mutation is the other central enzyme in cytoplasmic disulfide metabolism. Strains containing the trxB/gor mutations promote the highest levels of disulfide bonding in the E. coli cytoplasm (Prinz 1997). Expression of pET-32 constructs in a trxB/gor host may yield the maximum levels of soluble, active, properly folded target protein.



pET-32 is also available in two additional versions prepared for ligation-independent cloning (LIC) of PCR products. With these versions, all vector-encoded N-terminal fusion sequences can be removed from purified proteins with either enterokinase (Ek/LIC) or Factor Xa (Xa/LIC).

Another pET vector with the Trx•Tag thioredoxin protein is pET-48b(+) (Cat. No. 71467-3), which has a human rhinovirus 3C (HRV 3C) protease site for removal of the Trx•Tag fusion.






Commercial use of this Product requires a commercial license from EMD Millipore Corporation. Commercial use shall include but not be limited to (1) use of the Product or its components in manufacturing; (2) use of the Product or its components to provide a service, information, or data to others in exchange for consideration; (3) use of the Product or its components (or any derivatives thereof) for therapeutic, diagnostic or prophylactic purposes (including as part of a device, chip, assay or other product); or (4) resale of the Product or its components, whether or not such Product or its components are resold for use in research. Nothing contained herein shall be deemed to represent or warrant that additional third party rights are not required for use of this Product.
Catalogue Number70785
Brand Family Novagen®
References
References

Harrison, R.G. 2000. inNovations 11, 4. Davis, G.D., et al. 1999. Biotechnol. Bioeng. 65, 382. Novy, R. and Drott, D. 2002. inNovations 14, 12.
Product Information
•10 µg eachpET-32a(+), pET-32b(+), and pET-32c(+) vector DNAs
•0.2 ml eachBL21, BL21(DE3), BL21(DE3)pLysS, Origami 2(DE3), and Origami 2(DE3)pLysS glycerol stocks
•0.2 mlInduction Control
Fusion tagHis•Tag, S•Tag
Applications
Biological Information
Physicochemical Information
Dimensions
Materials Information
Toxicological Information
Safety Information according to GHS
Safety Information
Product Usage Statements
Storage and Shipping Information
Ship Code Shipped with Blue Ice or with Dry Ice
Toxicity Standard Handling
Storage ≤ -70°C
Avoid freeze/thaw Avoid freeze/thaw
Do not freeze Ok to freeze
Packaging Information
Transport Information
Supplemental Information
Specifications
Global Trade Item Number
Katalogové číslo GTIN
70785 0

Documentation

pET Trx Fusion System 32 - Novagen Certificates of Analysis

TitleLot Number
70785

References

Přehled odkazů

Harrison, R.G. 2000. inNovations 11, 4. Davis, G.D., et al. 1999. Biotechnol. Bioeng. 65, 382. Novy, R. and Drott, D. 2002. inNovations 14, 12.

Citations

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    • User Protocols

    Title
    TB053 Academic and Non-profit Laboratory Assurance Letter
    TB055 pET System Manual

    Vector Map

    Title
    TB122VM pET-32a-c(+) Vector Map