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71125 pET NusA Fusion System 44

pET vectors plus host strains with induction control

pET NusA Fusion System 44 MSDS (material safety data sheet) or SDS, CoA and CoQ, dossiers, brochures and other available documents.
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Description
Overview

This product has been discontinued.





pET Expression Systems and pET
pET Expression Systems and pET Expression Systems plus Competent Cells provide core reagents needed for target gene cloning and expression.

Components: pET Expression Systems
Components for pET Expression Systems are similar for all systems unless otherwise stated with the specific pET Expression System description. pET Expression Systems include:
• 10 µg pET vector DNA (for each indicated plasmid)
• 0.2 ml BL21 Glycerol Stock
• 0.2 ml BL21(DE3) Glycerol Stock
• 0.2 ml BL21(DE3)pLysS Glycerol Stock
• 0.2 ml Induction Control Glycerol Stock

Components: pET Expression Systems plus Competent Cells
pET Expression Systems plus Competent Cells contain all of the components of the specific pET Expression System, as well as the following additional components, unless otherwise stated with the specific pET Expression System description:
• 0.2 ml NovaBlue Competent Cells
• 0.2 ml BL21(DE3) Competent Cells
• 0.2 ml BL21(DE3)pLysS Competent Cells
• 2 × 0.2 ml SOC Medium
• 10 µl Test Plasmid

These components are sufficient for up to 10 transformations in each host.

Purification and Detection Reagents
Purification and detection reagents are available separately. For complete product descriptions and ordering information, refer to the Protein Purification and Antibodies, Conjugates & Detection Tools chapters.
pET NusA Fusion System 44
The pET-44a-c(+) vectors, which are derived from the corresponding pET-43.1 series, encode an additional N-terminal His•Tag sequence for enhanced yields when purifying a target protein with His•Mag™ Agarose Beads from lysates generated with the BugBuster® HT Protein Extraction Reagent and PopCulture® Reagent (Novy 2002). N-terminal S•Tag and optional C-terminal HSV•Tag and His•Tag fusion sequences are also available for detection and purification of target proteins. The pET NusA Fusion System 44 is designed for cloning and high-level expression of polypeptide sequences fused with the 495 aa NusA (Nus•Tag™) protein. The solubility of many target proteins in the E. coli cytoplasm is increased when they are expressed NusA fusions. The pET-44a-c(+) vectors, which are derived from the corresponding pET-43.1 series, encode an additional N-terminal His•Tag® sequence for enhanced yields when purifying a target protein with His•Mag Agarose Beads from lysates generated with the BugBuster HT Reagent and PopCulture Reagent. N-terminal S•Tag™ and optional C-terminal HSV•Tag® and His•Tag fusion sequences are also available for versatile detection and purification of target proteins.






Commercial use of this Product requires a commercial license from EMD Millipore Corporation. Commercial use shall include but not be limited to (1) use of the Product or its components in manufacturing; (2) use of the Product or its components to provide a service, information, or data to others in exchange for consideration; (3) use of the Product or its components (or any derivatives thereof) for therapeutic, diagnostic or prophylactic purposes (including as part of a device, chip, assay or other product); or (4) resale of the Product or its components, whether or not such Product or its components are resold for use in research. Nothing contained herein shall be deemed to represent or warrant that additional third party rights are not required for use of this Product.
Catalogue Number71125
Brand Family Novagen®
References
References

Harrison, R.G. 2000. inNovations 11, 4. Davis, G.D., et al. 1999. Biotechnol. Bioeng. 65, 382. Novy, R. and Drott, D. 2002. inNovations 14, 12.
Product Information
Components
Applications
Biological Information
Physicochemical Information
Dimensions
Materials Information
Toxicological Information
Safety Information according to GHS
Safety Information
Product Usage Statements
Storage and Shipping Information
Ship Code Shipped with Blue Ice or with Dry Ice
Toxicity Standard Handling
Storage ≤ -70°C
Do not freeze Ok to freeze
Packaging Information
Transport Information
Supplemental Information
Specifications
Global Trade Item Number
Katalogové číslo GTIN
71125 0

Documentation

pET NusA Fusion System 44 Certificates of Analysis

TitleLot Number
71125

References

Přehled odkazů

Harrison, R.G. 2000. inNovations 11, 4. Davis, G.D., et al. 1999. Biotechnol. Bioeng. 65, 382. Novy, R. and Drott, D. 2002. inNovations 14, 12.

Citations

Název
  • Kyung Uk Hong, et al. (2007) Functional importance of the anaphase-promoting complex-Cdh1-mediated degradation of TMAP/CKAP2 in regulation of spindle function and cytokinesis. Molecular and Cellular Biology 27, 3667-3681.
  • Masakazu Agetsuma, et al. (2005) The ubiquitin-proteasome pathway is involved in rapid degradation of phosphoenolpyruvate carboxylase kinase for C4 photosynthesis. Plant and Cell Physiology 46, (389- 398).
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    User Protocols

    Title
    TB053 Academic and Non-profit Laboratory Assurance Letter
    TB055 pET System Manual

    Vector Map

    Title
    TB330VM pET-44a-c(+) Vector Map