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			96-Well Plate

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	48-602MAG	Buffer Detection Kit for Magnetic Beads	1 Kit

70771 pET Expression System 25b

pET vectors plus host strains with induction control

pET Expression System 25b MSDS (material safety data sheet) or SDS, CoA and CoQ, dossiers, brochures and other available documents.

CoA
User Protocol
Citations
Vector Map

Recommended Products

Přehled

Replacement Information

Description
Overview	This product has been discontinued. pET Expression Systems and pET pET Expression Systems and pET Expression Systems plus Competent Cells provide core reagents needed for target gene cloning and expression. Components: pET Expression Systems Components for pET Expression Systems are similar for all systems unless otherwise stated with the specific pET Expression System description. pET Expression Systems include: • 10 µg pET vector DNA (for each indicated plasmid) • 0.2 ml BL21 Glycerol Stock • 0.2 ml BL21(DE3) Glycerol Stock • 0.2 ml BL21(DE3)pLysS Glycerol Stock • 0.2 ml Induction Control Glycerol Stock Components: pET Expression Systems plus Competent Cells pET Expression Systems plus Competent Cells contain all of the components of the specific pET Expression System, as well as the following additional components, unless otherwise stated with the specific pET Expression System description: • 0.2 ml NovaBlue Competent Cells • 0.2 ml BL21(DE3) Competent Cells • 0.2 ml BL21(DE3)pLysS Competent Cells • 2 × 0.2 ml SOC Medium • 10 µl Test Plasmid These components are sufficient for up to 10 transformations in each host. Purification and Detection Reagents Purification and detection reagents are available separately. For complete product descriptions and ordering information, refer to the Protein Purification and Antibodies, Conjugates & Detection Tools chapters. pET Expression System 25b The pET-25b(+) vector carries an N-terminal pelB signal sequence for potential periplasmic localization, plus optional C-terminal HSV•Tag^® and His•Tag^® sequence. Note that the sequence is numbered by the pBR322 convention, so the T7 expression region is reversed on the vector map, TB065VM. Commercial use of this Product requires a commercial license from EMD Millipore Corporation. Commercial use shall include but not be limited to (1) use of the Product or its components in manufacturing; (2) use of the Product or its components to provide a service, information, or data to others in exchange for consideration; (3) use of the Product or its components (or any derivatives thereof) for therapeutic, diagnostic or prophylactic purposes (including as part of a device, chip, assay or other product); or (4) resale of the Product or its components, whether or not such Product or its components are resold for use in research. Nothing contained herein shall be deemed to represent or warrant that additional third party rights are not required for use of this Product.
Catalogue Number	70771
Brand Family	Novagen®

Description

Overview

This product has been discontinued.

pET Expression Systems and pET
pET Expression Systems and pET Expression Systems plus Competent Cells provide core reagents needed for target gene cloning and expression.

Components: pET Expression Systems
Components for pET Expression Systems are similar for all systems unless otherwise stated with the specific pET Expression System description. pET Expression Systems include:
• 10 µg pET vector DNA (for each indicated plasmid)
• 0.2 ml BL21 Glycerol Stock
• 0.2 ml BL21(DE3) Glycerol Stock
• 0.2 ml BL21(DE3)pLysS Glycerol Stock
• 0.2 ml Induction Control Glycerol Stock

Components: pET Expression Systems plus Competent Cells
pET Expression Systems plus Competent Cells contain all of the components of the specific pET Expression System, as well as the following additional components, unless otherwise stated with the specific pET Expression System description:
• 0.2 ml NovaBlue Competent Cells
• 0.2 ml BL21(DE3) Competent Cells
• 0.2 ml BL21(DE3)pLysS Competent Cells
• 2 × 0.2 ml SOC Medium
• 10 µl Test Plasmid

These components are sufficient for up to 10 transformations in each host.

Purification and Detection Reagents
Purification and detection reagents are available separately. For complete product descriptions and ordering information, refer to the Protein Purification and Antibodies, Conjugates & Detection Tools chapters.

pET Expression System 25b
The pET-25b(+) vector carries an N-terminal pelB signal sequence for potential periplasmic localization, plus optional C-terminal HSV•Tag^® and His•Tag^® sequence. Note that the sequence is numbered by the pBR322 convention, so the T7 expression region is reversed on the vector map, TB065VM.

Commercial use of this Product requires a commercial license from EMD Millipore Corporation. Commercial use shall include but not be limited to (1) use of the Product or its components in manufacturing; (2) use of the Product or its components to provide a service, information, or data to others in exchange for consideration; (3) use of the Product or its components (or any derivatives thereof) for therapeutic, diagnostic or prophylactic purposes (including as part of a device, chip, assay or other product); or (4) resale of the Product or its components, whether or not such Product or its components are resold for use in research. Nothing contained herein shall be deemed to represent or warrant that additional third party rights are not required for use of this Product.

Catalogue Number

70771

Brand Family

Novagen®

References

Product Information
•	10 µg	pET-25b(+) vector DNA
•	0.2 ml	Host bacterial strains BL21, BL21(DE3), and BL21(DE3)pLysS, glycerol stocks
•	0.2 ml	Induction control clone, glycerol stock
Fusion tag	His•Tag

Applications

Biological Information

Physicochemical Information

Dimensions

Materials Information

Toxicological Information

Safety Information according to GHS

Safety Information

Product Usage Statements

Storage and Shipping Information
Ship Code	Shipped with Blue Ice or with Dry Ice
Toxicity	Standard Handling
Storage	≤ -70°C
Avoid freeze/thaw	Avoid freeze/thaw
Do not freeze	Ok to freeze

Packaging Information

Transport Information

Supplemental Information

Specifications

Global Trade Item Number
Katalogové číslo	GTIN
70771	0

Documentation

pET Expression System 25b Certificates of Analysis

Title	Lot Number
70771	Zadejte číslo šarže

Citations

Název

Gene L. Bidwell and Drazen Raucher. (2005) Application of thermally responsive polypeptides directed against c-Myc transcriptional function for cancer therapy. Molecular Cancer Therapeutics 4, 1076-1085.

Nikola Kostlánová, et al. (2005) The fucose-binding lectin from Ralstonia solanacearum: A new type of β-propeller architecture formed by oligomerisation and interacting with fucoside, fucosyllactose and plant xyloglucan. Journal of Biological Chemistry 280, 27839-27849.

Hao Zhou, Wei Wang and Yongzhang Luo. (2005) Contributions of disulfide bonds in a nested pattern to the structure, stability, and biological functions of endostatin. Journal of Biological Chemistry 280, 11303-11312.

K. Trabbic-Carlson, et al. (2004) Effect of protein fusion on the transition temperature of an environmentally responsive elastin-like polypeptide: a role for surface hydrophobicity? Protein Engineering Design and Selection 17, 57-66.

P. M. Deckert, et al. (2003) A33scFv-cytosine deaminase: a recombinant protein construct for antibody-directed enzyme-prodrug therapy. British Journal of Cancer 88, 937-939.

Songmin Cai and John H. Exton. (2001) Determination of interaction sites of phospholipase D1 for RhoA. Biochemical Journal 355, 779-785.

Kerri Cooper and Francois Baneyx. (2001) Escherichia coli FtsH (HflB) degrades a membrane-associated TolAI-II-βlactamase fusion protein under highly denaturing conditions. Protein Expression and Purification 21, 323-332.

Michael W. West, et al. (1999) De novo amyloid proteins from designed combinatorial libraries. Procedings of the National Academy of Science 96, 1121111216.

L.L. Murley and N.D.F. Grindley. (1998) Architecture of the γδ resolvase synaptosome: oriented heterodimers identify interactions essential for synapsis and recombination. Cell 95, 553-562.

Paul Zhou, et al. (1998) Cells transfected with a non-neutralizing antibody gene are resistant to HIV infection: trageting the endoplasmic reticulum and trans-golgi network. Journal of Immunology 160, 1439-1496.

User Protocols

Title
TB053 Academic and Non-profit Laboratory Assurance Letter
TB055 pET System Manual

Vector Map

Title
TB065 pET-25b(+) Vector Map

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Související produkty: Application Facete

Kategorie

Life Science Research > Genomic Analysis > Transfection and Protein Expression > Bacterial Expression > Bacterial Expression Vectors

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