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QIA117 Senescence Detection Kit

Senescence Detection Kit MSDS (material safety data sheet) or SDS, CoA and CoQ, dossiers, brochures and other available documents.
QIA117
  
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Přehled

Replacement Information

Tabulka spec. kláve

Detection Methods
Colorimetric
Description
OverviewUseful for the histochemical detection of senescent cells. The failure of cells to become senescent may lead to tumor progression. Higher levels of β-Galactosidase is considered as a good marker for senescent cells. The mechanism of this is believed to be due to the increased lysosomal content of senescent cells, resulting in an increase of the lysosomal enzyme β-galactosidase. Cells can be observed using microscope.
Catalogue NumberQIA117
Brand Family Calbiochem®
Materials Required but Not Delivered• PBS
• DMF
References
ReferencesKurz, D.J., et al. 2000. J. Cell Sci. 113, 3613.
Dimri, G.P., et al. 1995. Proc. Natl. Acad. Sci. USA 92, 9363.
Product Information
Detection methodColorimetric
Form250 Tests
Format12-well plate
Kit containsFixative Solution, X-Gal, Staining Solution, Staining Supplement, and a user protocol.
Quality LevelMQ100
Applications
Application ReferencesFunasaka, T., et al. 2007. J. Biol. Chem. 282, 36362. Chen, Z., et al. 2005. Nature 436, 725.
Biological Information
Assay time30 min. plus overnight incubation
Sample TypeIntact cells
Physicochemical Information
Dimensions
Materials Information
Toxicological Information
Safety Information according to GHS
Safety Information
Product Usage Statements
Intended useThe Calbiochem® Senescence Detection Kit is useful for the histochemical detection of senescent cells. The failure of cells to become senescent may lead to tumor progression. The mechanism of this is believed to be the increased lysosomal content of senescent cells resulting in an increase of the lysosomal enzyme β-galactosidase.
Storage and Shipping Information
Ship Code Dry Ice Only
Toxicity Multiple Toxicity Values, refer to MSDS
Storage -20°C
Storage ConditionsUpon arrival store the entire contents of the kit at -20°C.
Protect from Light Protect from light
Do not freeze Ok to freeze
Packaging Information
Transport Information
Supplemental Information
Kit containsFixative Solution, X-Gal, Staining Solution, Staining Supplement, and a user protocol.
Specifications
Global Trade Item Number
Katalogové číslo GTIN
QIA117 0

Documentation

Senescence Detection Kit MSDS

Title

Safety Data Sheet (SDS) 

Senescence Detection Kit Certificates of Analysis

TitleLot Number
QIA117

References

Přehled odkazů
Kurz, D.J., et al. 2000. J. Cell Sci. 113, 3613.
Dimri, G.P., et al. 1995. Proc. Natl. Acad. Sci. USA 92, 9363.
User Protocol

Revision07-August-2008 JSW
Form250 Tests
Format12-well plate
Detection methodColorimetric
StorageUpon arrival store the entire contents of the kit at -20°C.
Intended useThe Calbiochem® Senescence Detection Kit is useful for the histochemical detection of senescent cells. The failure of cells to become senescent may lead to tumor progression. The mechanism of this is believed to be the increased lysosomal content of senescent cells resulting in an increase of the lysosomal enzyme β-galactosidase.
BackgroundSenescence is thought to be a tumor suppressive mechanism and an underlying cause of aging. It represents an arrested state in which the cells remain viable, but not stimulated to divide by serum or by passage in culture. Senescent cells display an increase in cell size, pH-dependent expression of β-galactosidase (β-Gal) activity, and altered patterns of gene expression.
Principles of the assayThe Calbiochem® Senescence Detection Kit is designed to histochemically detect β-Gal activity in cultured cells and in tissue sections at pH 6.0, which is a known characteristic of senescent cells. β-Gal at pH 6.0 is present only in senescent cells and is not found in presenescent, quiescent, or immortal cells.
Materials provided• Fixative Solution (Kit Component No. JA7633-125ML): 1 vial, 125 ml
• X-Gal (Kit Component No. JA7634-150MG): 1 vial, 150 mg
• Staining Solution (Kit Component No. JA7635-125ML): 1 vial, 125 ml
• Staining Supplement (Kit Component No. JA7636-1.5ML): 1 vial, 1.5 ml
Materials Required but not provided• PBS
• DMF
Reagent preparation• PBS: Prepare a 1X PBS solution (not provided). Use at least 3 ml per well. • X-gal: Dissolve 20 mg X-gal in 1 ml of DMF (N-N-dimethylformamide, not provided) to prepare a 20X stock solution. Aliquot and freeze (-20°) the unused X-gal solution. This stock solution is stable for up to 1 month at -20°C (protect from light). Always store X-gal in a polypropylene or glass container. Do not use polystyrene. • Staining Solution Mix: While cells are incubating in Fixative Solution (step 2 below), prepare Staining Solution Mix in a polypropylene plastic tube by adding 470 µl of Staining Solution, 5 µl Staining Supplement, and 25 µl of 20 mg/ml X-gal in DMF.
Detailed protocolStaining

Note: this procedure is described for one well of a 12-well culture plate. For a large plate, increase the volume proportionally. Please review the entire procedure before starting experiments.

1.Remove culture medium and wash cells once with 1 ml 1X PBS.
2. Fix cells with 0.5 ml of Fixative Solution at room temperature for 10 to 15 min.
3. Wash the cells twice with 1 ml 1X PBS.
4. Add 0.5 ml Staining Solution Mix to each well. Incubate at 37°C overnight.
5. Observe cells under a microscope for development of blue color (approximately 200X total magnification).
6. For long-term storage of the stained plates, remove the staining solution mix and overlay the cells with 70% glycerol. Store at 4°C.
Application referencesFunasaka, T., et al. 2007. J. Biol. Chem. 282, 36362. Chen, Z., et al. 2005. Nature 436, 725.
Registered TrademarksCalbiochem® is a registered trademark of EMD Chemicals, Inc.
Interactive Pathways™ is a trademark of EMD Chemicals, Inc.